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Article: Performance of the VITEK MS matrix-assisted laser desorption ionization-time of flight mass spectrometry system for rapid bacterial identification in two diagnostic centres in China

TitlePerformance of the VITEK MS matrix-assisted laser desorption ionization-time of flight mass spectrometry system for rapid bacterial identification in two diagnostic centres in China
Authors
Issue Date2015
PublisherSociety for General Microbiology. The Journal's web site is located at http://jmm.sgmjournals.org
Citation
Journal of Medical Microbiology, 2015, v. 64 n. 1, p. 18-24 How to Cite?
AbstractMatrix-assisted laser desorption ionization-time of flight (MALDI-TOF) MS systems was not officially launched for diagnostic use in clinical microbiology laboratories in China until 2012. Here, we report the findings from the first large-scale evaluation study of VITEK MS for routine bacterial identification in two major diagnostic centres in Beijing and Hong Kong. A total of 2266 unique isolates representing 56 genera and 127 species were analysed, and results were compared to those obtained by VITEK 2. Any discrepancies were resolved by 16S rRNA sequencing. Overall, VITEK MS provided correct identification for 2246 (99.1%) isolates, including 2193 (96.8%) with correct species-level identifications and 53 (2.3%) matched at the genus level only. VITEK MS surpassed VITEK 2 consistently in species-level identification of important pathogens, including non-Enterobacteriaceae Gram-negative bacilli (94.7 versus 92%), staphylococci (99.7 versus 92.4%), streptococci (92.6 versus 79.4%), enterococci (98.8 versus 92.6%) and Clostridium spp. (97.3 versus 55.5%). The findings demonstrated that VITEK MS is highly accurate and reliable for routine bacterial identification in clinical settings in China. © 2015 The Authors.
Persistent Identifierhttp://hdl.handle.net/10722/220170
ISSN
2015 Impact Factor: 2.269
2015 SCImago Journal Rankings: 1.060
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLuo, Y-
dc.contributor.authorSiu, GKH-
dc.contributor.authorYeung, ASF-
dc.contributor.authorChen, JHK-
dc.contributor.authorHo, PL-
dc.contributor.authorLeung, KW-
dc.contributor.authorTsang, LY-
dc.contributor.authorCheng, VCC-
dc.contributor.authorGuo, L-
dc.contributor.authorYang, J-
dc.contributor.authorYe, L-
dc.contributor.authorYam, WC-
dc.date.accessioned2015-10-16T06:31:27Z-
dc.date.available2015-10-16T06:31:27Z-
dc.date.issued2015-
dc.identifier.citationJournal of Medical Microbiology, 2015, v. 64 n. 1, p. 18-24-
dc.identifier.issn0022-2615-
dc.identifier.urihttp://hdl.handle.net/10722/220170-
dc.description.abstractMatrix-assisted laser desorption ionization-time of flight (MALDI-TOF) MS systems was not officially launched for diagnostic use in clinical microbiology laboratories in China until 2012. Here, we report the findings from the first large-scale evaluation study of VITEK MS for routine bacterial identification in two major diagnostic centres in Beijing and Hong Kong. A total of 2266 unique isolates representing 56 genera and 127 species were analysed, and results were compared to those obtained by VITEK 2. Any discrepancies were resolved by 16S rRNA sequencing. Overall, VITEK MS provided correct identification for 2246 (99.1%) isolates, including 2193 (96.8%) with correct species-level identifications and 53 (2.3%) matched at the genus level only. VITEK MS surpassed VITEK 2 consistently in species-level identification of important pathogens, including non-Enterobacteriaceae Gram-negative bacilli (94.7 versus 92%), staphylococci (99.7 versus 92.4%), streptococci (92.6 versus 79.4%), enterococci (98.8 versus 92.6%) and Clostridium spp. (97.3 versus 55.5%). The findings demonstrated that VITEK MS is highly accurate and reliable for routine bacterial identification in clinical settings in China. © 2015 The Authors.-
dc.languageeng-
dc.publisherSociety for General Microbiology. The Journal's web site is located at http://jmm.sgmjournals.org-
dc.relation.ispartofJournal of Medical Microbiology-
dc.rightsJournal of Medical Microbiology. Copyright © Society for General Microbiology.-
dc.rightsThis is an author manuscript that has been accepted for publication in Journal of Medical Microbiology, copyright Society for General Microbiology, but has not been copy-edited, formatted or proofed. Cite this article as appearing in Journal of Medical Microbiology. This version of the manuscript may not be duplicated or reproduced, other than for personal use or within the rule of 'Fair Use of Copyrighted Materials' (section 17, Title 17, US Code), without permission from the copyright owner, Society for General Microbiology. The Society for General Microbiology disclaims any responsibility or liability for errors or omissions in this version of the manuscript or in any version derived from it by any other parties. The final copy-edited, published article, which is the version of record, can be found at http://dx.doi.org/10.1099/jmm.0.080317-0, and is freely available without a subscription.-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.titlePerformance of the VITEK MS matrix-assisted laser desorption ionization-time of flight mass spectrometry system for rapid bacterial identification in two diagnostic centres in China-
dc.typeArticle-
dc.identifier.emailSiu, GKH: gilman06@hkucc.hku.hk-
dc.identifier.emailChen, JHK: jonchk@hku.hk-
dc.identifier.emailHo, PL: plho@hkucc.hku.hk-
dc.identifier.emailCheng, VCC: vcccheng@hkucc.hku.hk-
dc.identifier.emailYam, WC: wcyam@hkucc.hku.hk-
dc.identifier.authorityHo, PL=rp00406-
dc.identifier.authorityYam, WC=rp00313-
dc.description.naturepostprint-
dc.identifier.doi10.1099/jmm.0.080317-0-
dc.identifier.pmid25418737-
dc.identifier.scopuseid_2-s2.0-84919772779-
dc.identifier.hkuros255208-
dc.identifier.volume64-
dc.identifier.issue1-
dc.identifier.spage18-
dc.identifier.epage24-
dc.identifier.isiWOS:000355787400003-
dc.publisher.placeUnited Kingdom-

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