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Article: Diffusion magnetic resonance monitors intramyocellular lipid droplet size in vivo

TitleDiffusion magnetic resonance monitors intramyocellular lipid droplet size in vivo
Authors
KeywordsIntramyocellular lipid
Obesity
Streptozotocininduced diabetes
Intracellular lipid
Fasting
Diffusion magnetic resonance spectroscopy
Issue Date2015
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0740-3194/
Citation
Magnetic Resonance in Medicine, 2015, v. 73 n. 1, p. 59-69 How to Cite?
Abstract© 2014 Wiley Periodicals, Inc. Purpose: Intramyocellular lipid (IMCL) droplets are dynamic organelles whose morphology reflects their vital roles in lipid synthesis, usage, and storage in muscle energy metabolism. To develop noninvasive means to measure droplet microstructure in vivo, we investigated the molecular diffusion behavior of IMCL with diffusion magnetic resonance spectroscopy.Methods: Using extremely large diffusion weighting, we measured the IMCL apparent diffusion coefficients (ADCs) in hindlimb muscle of rodents from normal feeding, 60-h fasting, streptozotocin-induced diabetic, and high-fat-diet-induced obese groups.Results: IMCL ADCs decreased markedly with diffusion time, confirming the restricted diffusion of lipid molecules within IMCL droplets. IMCL droplet size, determined by transmission electron microscopy, was closely correlated with ADC. IMCL ADC was sensitive to metabolic alterations, decreasing in the 60-h fasting and diabetic groups while increasing in the obese group. These findings indicated that the IMCL droplet size decreased following 60-h fasting and in STZinduced diabetes but increased in high-fat-diet-induced obesity.Conclusion: MR diffusion characterization of IMCL droplet size provides a unique means to examine the intracellular lipid dynamics and metabolic abnormalities in vivo.
Persistent Identifierhttp://hdl.handle.net/10722/220147
ISSN
2017 Impact Factor: 4.082
2015 SCImago Journal Rankings: 2.197
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorWu, EX-
dc.contributor.authorCao, P-
dc.contributor.authorFan, SJ-
dc.contributor.authorWang, AM-
dc.contributor.authorXie, VB-
dc.contributor.authorQiao, ZW-
dc.contributor.authorBrittenham, GM-
dc.date.accessioned2015-10-16T06:30:36Z-
dc.date.available2015-10-16T06:30:36Z-
dc.date.issued2015-
dc.identifier.citationMagnetic Resonance in Medicine, 2015, v. 73 n. 1, p. 59-69-
dc.identifier.issn0740-3194-
dc.identifier.urihttp://hdl.handle.net/10722/220147-
dc.description.abstract© 2014 Wiley Periodicals, Inc. Purpose: Intramyocellular lipid (IMCL) droplets are dynamic organelles whose morphology reflects their vital roles in lipid synthesis, usage, and storage in muscle energy metabolism. To develop noninvasive means to measure droplet microstructure in vivo, we investigated the molecular diffusion behavior of IMCL with diffusion magnetic resonance spectroscopy.Methods: Using extremely large diffusion weighting, we measured the IMCL apparent diffusion coefficients (ADCs) in hindlimb muscle of rodents from normal feeding, 60-h fasting, streptozotocin-induced diabetic, and high-fat-diet-induced obese groups.Results: IMCL ADCs decreased markedly with diffusion time, confirming the restricted diffusion of lipid molecules within IMCL droplets. IMCL droplet size, determined by transmission electron microscopy, was closely correlated with ADC. IMCL ADC was sensitive to metabolic alterations, decreasing in the 60-h fasting and diabetic groups while increasing in the obese group. These findings indicated that the IMCL droplet size decreased following 60-h fasting and in STZinduced diabetes but increased in high-fat-diet-induced obesity.Conclusion: MR diffusion characterization of IMCL droplet size provides a unique means to examine the intracellular lipid dynamics and metabolic abnormalities in vivo.-
dc.languageeng-
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0740-3194/-
dc.relation.ispartofMagnetic Resonance in Medicine-
dc.subjectIntramyocellular lipid-
dc.subjectObesity-
dc.subjectStreptozotocininduced diabetes-
dc.subjectIntracellular lipid-
dc.subjectFasting-
dc.subjectDiffusion magnetic resonance spectroscopy-
dc.titleDiffusion magnetic resonance monitors intramyocellular lipid droplet size in vivo-
dc.typeArticle-
dc.identifier.emailWu, EX: ewu@eee.hku.hk-
dc.identifier.emailCao, P: caopeng1@hku.hk-
dc.identifier.authorityWu, EX=rp00193-
dc.identifier.authorityCao, P=rp02474-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/mrm.25116-
dc.identifier.pmid24469956-
dc.identifier.scopuseid_2-s2.0-84919871669-
dc.identifier.hkuros255266-
dc.identifier.volume73-
dc.identifier.issue1-
dc.identifier.spage59-
dc.identifier.epage69-
dc.identifier.isiWOS:000346908800007-
dc.publisher.placeUnited States-

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