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Article: Regulation of acetylcholine receptor clustering by ADF/cofilin-directed vesicular trafficking

TitleRegulation of acetylcholine receptor clustering by ADF/cofilin-directed vesicular trafficking
Authors
Issue Date2009
Citation
Nature Neuroscience, 2009, v. 12, n. 7, p. 848-856 How to Cite?
AbstractPostsynaptic receptor localization is crucial for synapse development and function, but the underlying cytoskeletal mechanisms remain elusive. Using Xenopus neuromuscular junctions as a model, we found that actin depolymerizing factor (ADF)/cofilin regulated actin-dependent vesicular trafficking of acetylcholine receptors (AChRs) to the postsynaptic membrane. Active ADF/cofilin was concentrated in small puncta adjacent to AChR clusters and was spatiotemporally correlated with the formation and maintenance of surface AChR clusters. Notably, increased actin dynamics, vesicular markers and intracellular AChRs were all enriched at the sites of ADF/cofilin localization. Furthermore, a substantial amount of new AChRs was detected at these ADF/cofilin-enriched sites. Manipulation of either ADF/cofilin activity through its serine-3 phosphorylation or ADF/cofilin localization via 14-3-3 proteins markedly attenuated AChR insertion and clustering. These results suggest that spatiotemporally restricted ADF/cofilin-mediated actin dynamics regulate AChR trafficking during the development of neuromuscular synapses. © 2009 Nature America, Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/219857
ISSN
2015 Impact Factor: 16.724
2015 SCImago Journal Rankings: 13.558

 

DC FieldValueLanguage
dc.contributor.authorLee, Chi Wai-
dc.contributor.authorHan, Jianzhong-
dc.contributor.authorBamburg, James R.-
dc.contributor.authorHan, Liang-
dc.contributor.authorLynn, Rachel-
dc.contributor.authorZheng, James Q.-
dc.date.accessioned2015-09-24T04:44:07Z-
dc.date.available2015-09-24T04:44:07Z-
dc.date.issued2009-
dc.identifier.citationNature Neuroscience, 2009, v. 12, n. 7, p. 848-856-
dc.identifier.issn1097-6256-
dc.identifier.urihttp://hdl.handle.net/10722/219857-
dc.description.abstractPostsynaptic receptor localization is crucial for synapse development and function, but the underlying cytoskeletal mechanisms remain elusive. Using Xenopus neuromuscular junctions as a model, we found that actin depolymerizing factor (ADF)/cofilin regulated actin-dependent vesicular trafficking of acetylcholine receptors (AChRs) to the postsynaptic membrane. Active ADF/cofilin was concentrated in small puncta adjacent to AChR clusters and was spatiotemporally correlated with the formation and maintenance of surface AChR clusters. Notably, increased actin dynamics, vesicular markers and intracellular AChRs were all enriched at the sites of ADF/cofilin localization. Furthermore, a substantial amount of new AChRs was detected at these ADF/cofilin-enriched sites. Manipulation of either ADF/cofilin activity through its serine-3 phosphorylation or ADF/cofilin localization via 14-3-3 proteins markedly attenuated AChR insertion and clustering. These results suggest that spatiotemporally restricted ADF/cofilin-mediated actin dynamics regulate AChR trafficking during the development of neuromuscular synapses. © 2009 Nature America, Inc. All rights reserved.-
dc.languageeng-
dc.relation.ispartofNature Neuroscience-
dc.titleRegulation of acetylcholine receptor clustering by ADF/cofilin-directed vesicular trafficking-
dc.typeArticle-
dc.description.natureLink_to_subscribed_fulltext-
dc.identifier.doi10.1038/nn.2322-
dc.identifier.pmid19483689-
dc.identifier.scopuseid_2-s2.0-67649822159-
dc.identifier.volume12-
dc.identifier.issue7-
dc.identifier.spage848-
dc.identifier.epage856-
dc.identifier.f10001161649-

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