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Conference Paper: Science-based holistic philosophy of Chinese Medicine Formula: reflected in cytotoxicity of Tian-xian Liquid (invitro) and the protein expression of p21, MMP-1 andMDR-1 in Human Colorectal Carcinoma (in vivo) in response to Tian-xian Liquid

TitleScience-based holistic philosophy of Chinese Medicine Formula: reflected in cytotoxicity of Tian-xian Liquid (invitro) and the protein expression of p21, MMP-1 andMDR-1 in Human Colorectal Carcinoma (in vivo) in response to Tian-xian Liquid
Authors
Issue Date2014
Citation
The 13th Meeting of Consortium for Globalization of Chinese Medicine (CGCM 2014), Beijing, China, 27-29 August 2014. How to Cite?
AbstractAIM: This study aims at investigating the holistic effects of three functional constituent fractions obtained from Tian-xian Liquid (TXL) contributing to anti-tumorigenicity of TXL in human colorectal carcinoma in vivo and in vitro. METHODOLOGY: TXL and its functional constituent fractions, including fractions of ethyl-acetate (EA), butanol (BU) and aqueous (WA), were prepared. Tissue-specific proliferative effects of TXL and its three functional constituent fractions on human colorectal carcinoma HT-29 cells and splenocytes were studied by using MTT assay. Protein levels of p21, MMP-1 and MDR-1 in a xenografted nude mice model were examined by Western blot analysis. RESULTS AND DISCUSSION: TXL exerted anti-tumorigenicity in human colorectal carcinoma with tissue-specific cytotoxicity, through the interactions among its different functional fractions. The anti-proliferative action of BU and EA fractions were tissue-specific, in which BU fraction was anti-proliferative to HT-29 cells but proliferative to mouse splenocytes, whereas EA fraction was anti-proliferative to splenocyte but with far less effect on HT-29 cells. Besides, the response of p21, MMP-1 and MDR-1 revealed that all functional fractions worked together to exhibit mutual accentuation (相須) and/or mutual enhancement (相使) effects to achieve anti-proliferation, anti-metastasis and reversion of multi-drug resistance in anti-tumorigenicity. BU fraction and EA fraction exerted mutual counteraction (相殺) and mutual suppression (相畏) in anti-proliferative effects on splenocytes, and WA fraction and EA fraction demonstrated mutual antagonism (相惡) in anti-proliferative effects on HT-29 cells. CONCLUSION: This is the first study to elucidate the science behind the holistic philosophy of Chinese Medicine formula for treating colon cancer, through investigating into anti-tumorigenicity of TXL and its functional constituent fractions. Further research in this area may give hints to the development of more tissue-specific effective drugs with minimal side effects for fighting against human colorectal carcinoma.
Persistent Identifierhttp://hdl.handle.net/10722/218064

 

DC FieldValueLanguage
dc.contributor.authorSze, CWS-
dc.contributor.authorLeigh, AB-
dc.contributor.authorLai, YM-
dc.contributor.authorNg, TB-
dc.contributor.authorTong, Y-
dc.date.accessioned2015-09-18T06:22:03Z-
dc.date.available2015-09-18T06:22:03Z-
dc.date.issued2014-
dc.identifier.citationThe 13th Meeting of Consortium for Globalization of Chinese Medicine (CGCM 2014), Beijing, China, 27-29 August 2014.-
dc.identifier.urihttp://hdl.handle.net/10722/218064-
dc.description.abstractAIM: This study aims at investigating the holistic effects of three functional constituent fractions obtained from Tian-xian Liquid (TXL) contributing to anti-tumorigenicity of TXL in human colorectal carcinoma in vivo and in vitro. METHODOLOGY: TXL and its functional constituent fractions, including fractions of ethyl-acetate (EA), butanol (BU) and aqueous (WA), were prepared. Tissue-specific proliferative effects of TXL and its three functional constituent fractions on human colorectal carcinoma HT-29 cells and splenocytes were studied by using MTT assay. Protein levels of p21, MMP-1 and MDR-1 in a xenografted nude mice model were examined by Western blot analysis. RESULTS AND DISCUSSION: TXL exerted anti-tumorigenicity in human colorectal carcinoma with tissue-specific cytotoxicity, through the interactions among its different functional fractions. The anti-proliferative action of BU and EA fractions were tissue-specific, in which BU fraction was anti-proliferative to HT-29 cells but proliferative to mouse splenocytes, whereas EA fraction was anti-proliferative to splenocyte but with far less effect on HT-29 cells. Besides, the response of p21, MMP-1 and MDR-1 revealed that all functional fractions worked together to exhibit mutual accentuation (相須) and/or mutual enhancement (相使) effects to achieve anti-proliferation, anti-metastasis and reversion of multi-drug resistance in anti-tumorigenicity. BU fraction and EA fraction exerted mutual counteraction (相殺) and mutual suppression (相畏) in anti-proliferative effects on splenocytes, and WA fraction and EA fraction demonstrated mutual antagonism (相惡) in anti-proliferative effects on HT-29 cells. CONCLUSION: This is the first study to elucidate the science behind the holistic philosophy of Chinese Medicine formula for treating colon cancer, through investigating into anti-tumorigenicity of TXL and its functional constituent fractions. Further research in this area may give hints to the development of more tissue-specific effective drugs with minimal side effects for fighting against human colorectal carcinoma.-
dc.languageeng-
dc.relation.ispartofMeeting of Consortium for Globalization of Chinese Medicine, CGCM 2014-
dc.relation.ispartof2014 第十三届中药全球化联盟研讨会-
dc.titleScience-based holistic philosophy of Chinese Medicine Formula: reflected in cytotoxicity of Tian-xian Liquid (invitro) and the protein expression of p21, MMP-1 andMDR-1 in Human Colorectal Carcinoma (in vivo) in response to Tian-xian Liquid-
dc.typeConference_Paper-
dc.identifier.emailSze, CWS: stephens@hku.hk-
dc.identifier.emailTong, Y: tongyao@hku.hk-
dc.identifier.authoritySze, CWS=rp00514-
dc.identifier.authorityTong, Y=rp00509-
dc.identifier.hkuros253105-
dc.publisher.placeBeijing-

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