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Article: Fission yeast mitochondria are distributed by dynamic microtubules in a motor-independent manner

TitleFission yeast mitochondria are distributed by dynamic microtubules in a motor-independent manner
Authors
Issue Date2015
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/srep/index.html
Citation
Scientific Reports, 2015, v. 5, article no. 11023 How to Cite?
AbstractThe cytoskeleton plays a critical role in regulating mitochondria distribution. Similar to axonal mitochondria, the fission yeast mitochondria are distributed by the microtubule cytoskeleton, but this is regulated by a motor-independent mechanism depending on the microtubule associated protein mmb1p as the absence of mmb1p causes mitochondria aggregation. In this study, using a series of chimeric proteins to control the subcellular localization and motility of mitochondria, we show that a chimeric molecule containing a microtubule binding domain and the mitochondria outer membrane protein tom22p can restore the normal interconnected mitochondria network in mmb1-deletion (mmb1∆) cells. In contrast, increasing the motility of mitochondria by using a chimeric molecule containing a kinesin motor domain and tom22p cannot rescue mitochondria aggregation defects in mmb1∆ cells. Intriguingly a chimeric molecule carrying an actin binding domain and tom22p results in mitochondria associated with actin filaments at the actomyosin ring during mitosis, leading to cytokinesis defects. These findings suggest that the passive motor-independent microtubule-based mechanism is the major contributor to mitochondria distribution in wild type fission yeast cells. Hence, we establish that attachment to microtubules, but not kinesin-dependent movement and the actin cytoskeleton, is required and crucial for proper mitochondria distribution in fission yeast.
Persistent Identifierhttp://hdl.handle.net/10722/216384
ISSN
2015 Impact Factor: 5.228
2015 SCImago Journal Rankings: 2.073
PubMed Central ID

 

DC FieldValueLanguage
dc.contributor.authorLi, T-
dc.contributor.authorZheng, F-
dc.contributor.authorCheung, MCH-
dc.contributor.authorWang, FS-
dc.contributor.authorFu, C-
dc.date.accessioned2015-09-18T05:25:57Z-
dc.date.available2015-09-18T05:25:57Z-
dc.date.issued2015-
dc.identifier.citationScientific Reports, 2015, v. 5, article no. 11023-
dc.identifier.issn2045-2322-
dc.identifier.urihttp://hdl.handle.net/10722/216384-
dc.description.abstractThe cytoskeleton plays a critical role in regulating mitochondria distribution. Similar to axonal mitochondria, the fission yeast mitochondria are distributed by the microtubule cytoskeleton, but this is regulated by a motor-independent mechanism depending on the microtubule associated protein mmb1p as the absence of mmb1p causes mitochondria aggregation. In this study, using a series of chimeric proteins to control the subcellular localization and motility of mitochondria, we show that a chimeric molecule containing a microtubule binding domain and the mitochondria outer membrane protein tom22p can restore the normal interconnected mitochondria network in mmb1-deletion (mmb1∆) cells. In contrast, increasing the motility of mitochondria by using a chimeric molecule containing a kinesin motor domain and tom22p cannot rescue mitochondria aggregation defects in mmb1∆ cells. Intriguingly a chimeric molecule carrying an actin binding domain and tom22p results in mitochondria associated with actin filaments at the actomyosin ring during mitosis, leading to cytokinesis defects. These findings suggest that the passive motor-independent microtubule-based mechanism is the major contributor to mitochondria distribution in wild type fission yeast cells. Hence, we establish that attachment to microtubules, but not kinesin-dependent movement and the actin cytoskeleton, is required and crucial for proper mitochondria distribution in fission yeast.-
dc.languageeng-
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/srep/index.html-
dc.relation.ispartofScientific Reports-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.titleFission yeast mitochondria are distributed by dynamic microtubules in a motor-independent manner-
dc.typeArticle-
dc.identifier.emailCheung, MCH: mcheung9@hku.hk-
dc.identifier.emailFu, C: chuanhai@hku.hk-
dc.identifier.authorityCheung, MCH=rp00245-
dc.identifier.authorityFu, C=rp01515-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1038/srep11023-
dc.identifier.pmid26046468-
dc.identifier.pmcidPMC4457142-
dc.identifier.hkuros250539-
dc.identifier.volume5-
dc.publisher.placeUnited Kingdom-

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