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Article: The effects of TGF-α, IL-1β and PDGF on fibroblast adhesion to ECM-derived matrix and KGF gene expression

TitleThe effects of TGF-α, IL-1β and PDGF on fibroblast adhesion to ECM-derived matrix and KGF gene expression
Authors
KeywordsGranulocyte-macrophage colony-stimulating factor
Transforming growth factor-α
Platelet-derived growth factor-BB
Keratinocyte growth factor
Interpenetrating network
Interleukin-1β
Issue Date2010
Citation
Biomaterials, 2010, v. 31, n. 9, p. 2542-2548 How to Cite?
AbstractThe goal of this study was to elucidate the control mechanisms by which exogenous proteins regulate keratinocyte growth factor (KGF) expression in fibroblasts adhered to differing substrates and thereby provide insights into both fundamental in vitro cell signaling and cell-biomaterial interaction research. A serum-free culture system in which cells maintained their proliferative capacity was established and employed. The addition of transforming growth factor- α (TGF-α), interleukin-1β (IL-1β) and platelet-derived growth factor-BB (PDGF-BB) individually showed no effect on KGF protein release, however, IL-1β addition led to increased KGF mRNA transcription, intracellular KGF protein synthesis, and granulocyte-macrophage colony-stimulating factor (GM-CSF) release. Intracellular KGF protein synthesis and extracellular release were enhanced when fibroblasts were treated with a combination of IL-1β and PDGF-BB which suggests KGF synthesis and release are largely regulated by synergistic mechanisms. Surface-bound fibronectin-derived ligands and individual exogenous proteins promoted fibroblast adhesion to semi-interpenetrating polymer networks (sIPNs) but did not stimulate KGF release despite enhancement of KGF mRNA transcription. Additionally, serum conditioning was found to have a significant impact on KGF synthesis and the subsequent mechanisms controlling KGF release. This study demonstrates that KGF release from fibroblasts is likely regulated by multiple mechanisms involving post-transcriptional and exocytic controls which may be impacted by the presence of serum and how serum is removed from the in vitro cell environment. © 2009 Elsevier Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/216204
ISSN
2015 Impact Factor: 8.387
2015 SCImago Journal Rankings: 3.565

 

DC FieldValueLanguage
dc.contributor.authorWang, Xintong-
dc.contributor.authorWaldeck, Heather-
dc.contributor.authorKao, Weiyuan J.-
dc.date.accessioned2015-08-25T10:22:25Z-
dc.date.available2015-08-25T10:22:25Z-
dc.date.issued2010-
dc.identifier.citationBiomaterials, 2010, v. 31, n. 9, p. 2542-2548-
dc.identifier.issn0142-9612-
dc.identifier.urihttp://hdl.handle.net/10722/216204-
dc.description.abstractThe goal of this study was to elucidate the control mechanisms by which exogenous proteins regulate keratinocyte growth factor (KGF) expression in fibroblasts adhered to differing substrates and thereby provide insights into both fundamental in vitro cell signaling and cell-biomaterial interaction research. A serum-free culture system in which cells maintained their proliferative capacity was established and employed. The addition of transforming growth factor- α (TGF-α), interleukin-1β (IL-1β) and platelet-derived growth factor-BB (PDGF-BB) individually showed no effect on KGF protein release, however, IL-1β addition led to increased KGF mRNA transcription, intracellular KGF protein synthesis, and granulocyte-macrophage colony-stimulating factor (GM-CSF) release. Intracellular KGF protein synthesis and extracellular release were enhanced when fibroblasts were treated with a combination of IL-1β and PDGF-BB which suggests KGF synthesis and release are largely regulated by synergistic mechanisms. Surface-bound fibronectin-derived ligands and individual exogenous proteins promoted fibroblast adhesion to semi-interpenetrating polymer networks (sIPNs) but did not stimulate KGF release despite enhancement of KGF mRNA transcription. Additionally, serum conditioning was found to have a significant impact on KGF synthesis and the subsequent mechanisms controlling KGF release. This study demonstrates that KGF release from fibroblasts is likely regulated by multiple mechanisms involving post-transcriptional and exocytic controls which may be impacted by the presence of serum and how serum is removed from the in vitro cell environment. © 2009 Elsevier Ltd. All rights reserved.-
dc.languageeng-
dc.relation.ispartofBiomaterials-
dc.subjectGranulocyte-macrophage colony-stimulating factor-
dc.subjectTransforming growth factor-α-
dc.subjectPlatelet-derived growth factor-BB-
dc.subjectKeratinocyte growth factor-
dc.subjectInterpenetrating network-
dc.subjectInterleukin-1β-
dc.titleThe effects of TGF-α, IL-1β and PDGF on fibroblast adhesion to ECM-derived matrix and KGF gene expression-
dc.typeArticle-
dc.description.natureLink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.biomaterials.2009.12.018-
dc.identifier.pmid20036421-
dc.identifier.scopuseid_2-s2.0-74449092219-
dc.identifier.volume31-
dc.identifier.issue9-
dc.identifier.spage2542-
dc.identifier.epage2548-

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