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Article: Monocyte inflammatory and matrix remodeling response modulated by grafted ECM-derived ligand concentration

TitleMonocyte inflammatory and matrix remodeling response modulated by grafted ECM-derived ligand concentration
Authors
KeywordsHuman monocyte
RGD
Receptor-ligand interactions
Matrix metalloproteinase
Interleukin-1beta
Issue Date2009
Citation
Journal of Biomedical Materials Research - Part A, 2009, v. 91, n. 3, p. 741-752 How to Cite?
AbstractLigands presented on biomaterials are a common method to facilitate and control the host response. In a gelatin and polyethylene glycol diacrylate (PEGdA) based semi-interpenetrating network (sIPN), the effects of extracellular matrix (ECM)-derived peptide amount on monocyte adhesion and subsequent protein and mRNA expression were examined. Peptide amount on the sIPN surface was controlled by varying the wt % ratio of the peptide-PEG grafted gelatin to PEGdA. We hypothesized that increasing bioactive peptide amount would modulate human blood-derived monocyte adhesion, cytokine expression, and gene regulation. Monocyte adhesion, release of gelatin degrading proteases matrix metalloprotease-2 (MMP-2), matrix metalloprotease-9 (MMP-9), and proinflammatory protein interleukin-1β (IL-1β), and mRNA expression of these proteins were evaluated. We found RGD-PEG grafted sIPNs with higher surface RGD concentrations showed increased adherent density. MMP-2 and IL-1β protein release was also influenced by the ligand concentration, as initial increase in protein concentration was observed at higher ligand concentrations. MMP-9 protein showed an initial increase that subsided then increased. A decreased IL-1β protein and mRNA expression was observed over time but MMP-2 mRNA was not detected at any time though MMP-2 protein concentrations showed an initial burst. Hence, monocyte behavior was modulated by surface ligand identity in tandem with ligand concentration. © 2008 Wiley Periodicals, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/216203
ISSN
2015 Impact Factor: 3.263
2015 SCImago Journal Rankings: 0.979

 

DC FieldValueLanguage
dc.contributor.authorChung, Amy S.-
dc.contributor.authorWaldeck, Heather-
dc.contributor.authorSchmidt, David R.-
dc.contributor.authorKao, Weiyuan John-
dc.date.accessioned2015-08-25T10:22:24Z-
dc.date.available2015-08-25T10:22:24Z-
dc.date.issued2009-
dc.identifier.citationJournal of Biomedical Materials Research - Part A, 2009, v. 91, n. 3, p. 741-752-
dc.identifier.issn1549-3296-
dc.identifier.urihttp://hdl.handle.net/10722/216203-
dc.description.abstractLigands presented on biomaterials are a common method to facilitate and control the host response. In a gelatin and polyethylene glycol diacrylate (PEGdA) based semi-interpenetrating network (sIPN), the effects of extracellular matrix (ECM)-derived peptide amount on monocyte adhesion and subsequent protein and mRNA expression were examined. Peptide amount on the sIPN surface was controlled by varying the wt % ratio of the peptide-PEG grafted gelatin to PEGdA. We hypothesized that increasing bioactive peptide amount would modulate human blood-derived monocyte adhesion, cytokine expression, and gene regulation. Monocyte adhesion, release of gelatin degrading proteases matrix metalloprotease-2 (MMP-2), matrix metalloprotease-9 (MMP-9), and proinflammatory protein interleukin-1β (IL-1β), and mRNA expression of these proteins were evaluated. We found RGD-PEG grafted sIPNs with higher surface RGD concentrations showed increased adherent density. MMP-2 and IL-1β protein release was also influenced by the ligand concentration, as initial increase in protein concentration was observed at higher ligand concentrations. MMP-9 protein showed an initial increase that subsided then increased. A decreased IL-1β protein and mRNA expression was observed over time but MMP-2 mRNA was not detected at any time though MMP-2 protein concentrations showed an initial burst. Hence, monocyte behavior was modulated by surface ligand identity in tandem with ligand concentration. © 2008 Wiley Periodicals, Inc.-
dc.languageeng-
dc.relation.ispartofJournal of Biomedical Materials Research - Part A-
dc.subjectHuman monocyte-
dc.subjectRGD-
dc.subjectReceptor-ligand interactions-
dc.subjectMatrix metalloproteinase-
dc.subjectInterleukin-1beta-
dc.titleMonocyte inflammatory and matrix remodeling response modulated by grafted ECM-derived ligand concentration-
dc.typeArticle-
dc.description.natureLink_to_subscribed_fulltext-
dc.identifier.doi10.1002/jbm.a.32259-
dc.identifier.pmid19051303-
dc.identifier.scopuseid_2-s2.0-70350337951-
dc.identifier.volume91-
dc.identifier.issue3-
dc.identifier.spage741-
dc.identifier.epage752-
dc.identifier.eissn1552-4965-

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