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Article: Fibroblasts regulate monocyte response to ECM-derived matrix: The effects on monocyte adhesion and the production of inflammatory, matrix remodeling, and growth factor proteins

TitleFibroblasts regulate monocyte response to ECM-derived matrix: The effects on monocyte adhesion and the production of inflammatory, matrix remodeling, and growth factor proteins
Authors
KeywordsInterleu-kin-1
Vascular endothelial growth factor
Monocytes/macrophages
Matrix metalloproteinase-2/ -9
Granulocyte-macrophage colony-stimulating factor
Fibroblast
Issue Date2009
Citation
Journal of Biomedical Materials Research - Part A, 2009, v. 89, n. 4, p. 841-853 How to Cite?
AbstractMonocytes/macrophages and fibroblasts are recruited to the injury site and orchestrate the host response and tissue repair. We have previously shown that polyethylene glycol (PEG)-ylated arginine-glycine-aspartic acid (RGD) sequence grafted onto an extracellular matrix (ECM)-based semi-interpenetrating network (sIPN) enhances monocyte adhesion, and modulates subsequent gene expression and release of inflammatory and matrix remodeling factors. In this study, we investigate the direct influence of fibroblasts on monocyte response to this ECM mimic. Key wound-healing factors in inflammation, matrix remodeling, and regeneration were analyzed to gain insight into the interrelated role of regulation in fibroblast-monocyte interaction. Interleukin-lalpha/-lbeta (IL-lα/-iβ), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), monocyte inflammatory protein-lalpha/-lbeta (MIP-lα/- iβ), transforming growth factor-alpha (TGF-α), monocyte chemoattractant factor (MCP-1), matrix metalloproteinase-2/-9 (MMP-2/-9), vascular endothelial growth factor (VEGF), granulocyte-macrophage colony-stimulating factor (GM- CSF) were analyzed. Fibroblasts decreased monocyte adhe sion onto the RGD-grafted sIPN while increasing monocyte GM-CSF on all surfaces over time except for on RGD and PHSRN-grafted sIPN at 96 h. Monocytes decreased initial fibroblast IL-lα and TGF-α, but drastically increased fibroblast MMP-2 and GM-CSF. Monocyte IL-iβ, TNF-α, MIP- lp, MCP-1, MMP-9, and GM-CSF expression was increased over time in the presence of all sIPNs, and when the sIPNs were immobilized with ligands, a down-regulation of fibroblast IL-iβ, MlP-lα, MIP-iβ compared with unmodified sIPN was observed. When the ligand immobilized was RGD, monocyte TGF-α, MIP-iβ, and VEGF expression was increased while monocyte GM-CSF was decreased at selected time points. These results showed a dynamic monocyte response to selected ECM components in the presence of fibroblasts. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res 89A: 841-853, 2009. © 2009 Wiley Periodicals, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/216200
ISSN
2015 Impact Factor: 3.263
2015 SCImago Journal Rankings: 0.979

 

DC FieldValueLanguage
dc.contributor.authorChung, Amy S.-
dc.contributor.authorKao, Weiyuan John-
dc.date.accessioned2015-08-25T10:22:23Z-
dc.date.available2015-08-25T10:22:23Z-
dc.date.issued2009-
dc.identifier.citationJournal of Biomedical Materials Research - Part A, 2009, v. 89, n. 4, p. 841-853-
dc.identifier.issn1549-3296-
dc.identifier.urihttp://hdl.handle.net/10722/216200-
dc.description.abstractMonocytes/macrophages and fibroblasts are recruited to the injury site and orchestrate the host response and tissue repair. We have previously shown that polyethylene glycol (PEG)-ylated arginine-glycine-aspartic acid (RGD) sequence grafted onto an extracellular matrix (ECM)-based semi-interpenetrating network (sIPN) enhances monocyte adhesion, and modulates subsequent gene expression and release of inflammatory and matrix remodeling factors. In this study, we investigate the direct influence of fibroblasts on monocyte response to this ECM mimic. Key wound-healing factors in inflammation, matrix remodeling, and regeneration were analyzed to gain insight into the interrelated role of regulation in fibroblast-monocyte interaction. Interleukin-lalpha/-lbeta (IL-lα/-iβ), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), monocyte inflammatory protein-lalpha/-lbeta (MIP-lα/- iβ), transforming growth factor-alpha (TGF-α), monocyte chemoattractant factor (MCP-1), matrix metalloproteinase-2/-9 (MMP-2/-9), vascular endothelial growth factor (VEGF), granulocyte-macrophage colony-stimulating factor (GM- CSF) were analyzed. Fibroblasts decreased monocyte adhe sion onto the RGD-grafted sIPN while increasing monocyte GM-CSF on all surfaces over time except for on RGD and PHSRN-grafted sIPN at 96 h. Monocytes decreased initial fibroblast IL-lα and TGF-α, but drastically increased fibroblast MMP-2 and GM-CSF. Monocyte IL-iβ, TNF-α, MIP- lp, MCP-1, MMP-9, and GM-CSF expression was increased over time in the presence of all sIPNs, and when the sIPNs were immobilized with ligands, a down-regulation of fibroblast IL-iβ, MlP-lα, MIP-iβ compared with unmodified sIPN was observed. When the ligand immobilized was RGD, monocyte TGF-α, MIP-iβ, and VEGF expression was increased while monocyte GM-CSF was decreased at selected time points. These results showed a dynamic monocyte response to selected ECM components in the presence of fibroblasts. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res 89A: 841-853, 2009. © 2009 Wiley Periodicals, Inc.-
dc.languageeng-
dc.relation.ispartofJournal of Biomedical Materials Research - Part A-
dc.subjectInterleu-kin-1-
dc.subjectVascular endothelial growth factor-
dc.subjectMonocytes/macrophages-
dc.subjectMatrix metalloproteinase-2/ -9-
dc.subjectGranulocyte-macrophage colony-stimulating factor-
dc.subjectFibroblast-
dc.titleFibroblasts regulate monocyte response to ECM-derived matrix: The effects on monocyte adhesion and the production of inflammatory, matrix remodeling, and growth factor proteins-
dc.typeArticle-
dc.description.natureLink_to_subscribed_fulltext-
dc.identifier.doi10.1002/jbm.a.32431-
dc.identifier.pmid19437738-
dc.identifier.scopuseid_2-s2.0-66249105019-
dc.identifier.volume89-
dc.identifier.issue4-
dc.identifier.spage841-
dc.identifier.epage853-
dc.identifier.eissn1552-4965-

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