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Article: Complement-mediated leukocyte adhesion on poly(etherurethane ureas) under shear stress in vitro

TitleComplement-mediated leukocyte adhesion on poly(etherurethane ureas) under shear stress in vitro
Authors
Issue Date1996
Citation
Journal of Biomedical Materials Research, 1996, v. 32, n. 1, p. 99-109 How to Cite?
AbstractBlood-contacting biomaterials may activate the complement cascade, thus promoting leukocyte adhesion to the biomaterial surface. We hypothesize that the extent of complement activation is modulated by biomaterial formulation and the presence of fluid shear stress. To investigate this hypothesis, we tested base poly(etherurethane ureas) formulated with or without Santowhite® antioxidant, a nucleophilic additive. We found that adherent leukocyte densities decreased with increasing shear stress. Moreover, leukocyte adhesion was decreased significantly further by Santowhite® additive under shear stress but not under static conditions. Monocytes showed a higher propensity for adhesion than did neutrophils under shear and static conditions. Under static conditions, adherent cells on the Santowhite®- containing polyurethane had a slightly more activated morphology than those on the base polyurethane. Cell adhesion under shear stress was significantly decreased when C3 or fibronectin was depleted from the suspension medium. Santowhite® additive increased Factor B adsorption to the test surface while shear stress increased Factor H adsorption. The combination of Santowhite® additive and shear stress increased the adsorption of both Factor B and Factor H and the serum protein S-terminal complement complex levels, but it did not further increase the state of activation of adherent cells. We conclude that leukocyte adhesion on poly(etherurethane urea) surfaces is sensitive to the levels of shear stress and that both C3 and fibronectin are required to maintain adhesion in the presence of shear stress. The low state of cellular activation and increased Factor H adsorption may explain the decreased adherent leukocyte density on the Santowhite®-containing polyurethane. | It has been observed that the extent of complement activation resulting from blood contacting biomaterials may be modulated by biomaterial formulation and the presence of fluid shear stress. This hypothesis is investigated by testing base poly(etherurethane ureas) formulated with or without Santowhite antioxidant, a nucleophilic additive. Adherent leukocyte densities were found to decrease with increasing shear stress, while leukocyte adhesion was further decreased by Santowhite additive under shear stress but not under static conditions. Adherent cells on the Santowhite containing polyurethane had a slightly more activated morphology than those on the base polyurethane under static conditions.
Persistent Identifierhttp://hdl.handle.net/10722/216147
ISSN
2004 Impact Factor: 3.652
2006 SCImago Journal Rankings: 0.474

 

DC FieldValueLanguage
dc.contributor.authorKao, Weiyuan John-
dc.contributor.authorSapatnekar, Suneeti-
dc.contributor.authorHiltner, Anne-
dc.contributor.authorAnderson, James M.-
dc.date.accessioned2015-08-25T10:21:52Z-
dc.date.available2015-08-25T10:21:52Z-
dc.date.issued1996-
dc.identifier.citationJournal of Biomedical Materials Research, 1996, v. 32, n. 1, p. 99-109-
dc.identifier.issn0021-9304-
dc.identifier.urihttp://hdl.handle.net/10722/216147-
dc.description.abstractBlood-contacting biomaterials may activate the complement cascade, thus promoting leukocyte adhesion to the biomaterial surface. We hypothesize that the extent of complement activation is modulated by biomaterial formulation and the presence of fluid shear stress. To investigate this hypothesis, we tested base poly(etherurethane ureas) formulated with or without Santowhite® antioxidant, a nucleophilic additive. We found that adherent leukocyte densities decreased with increasing shear stress. Moreover, leukocyte adhesion was decreased significantly further by Santowhite® additive under shear stress but not under static conditions. Monocytes showed a higher propensity for adhesion than did neutrophils under shear and static conditions. Under static conditions, adherent cells on the Santowhite®- containing polyurethane had a slightly more activated morphology than those on the base polyurethane. Cell adhesion under shear stress was significantly decreased when C3 or fibronectin was depleted from the suspension medium. Santowhite® additive increased Factor B adsorption to the test surface while shear stress increased Factor H adsorption. The combination of Santowhite® additive and shear stress increased the adsorption of both Factor B and Factor H and the serum protein S-terminal complement complex levels, but it did not further increase the state of activation of adherent cells. We conclude that leukocyte adhesion on poly(etherurethane urea) surfaces is sensitive to the levels of shear stress and that both C3 and fibronectin are required to maintain adhesion in the presence of shear stress. The low state of cellular activation and increased Factor H adsorption may explain the decreased adherent leukocyte density on the Santowhite®-containing polyurethane. | It has been observed that the extent of complement activation resulting from blood contacting biomaterials may be modulated by biomaterial formulation and the presence of fluid shear stress. This hypothesis is investigated by testing base poly(etherurethane ureas) formulated with or without Santowhite antioxidant, a nucleophilic additive. Adherent leukocyte densities were found to decrease with increasing shear stress, while leukocyte adhesion was further decreased by Santowhite additive under shear stress but not under static conditions. Adherent cells on the Santowhite containing polyurethane had a slightly more activated morphology than those on the base polyurethane under static conditions.-
dc.languageeng-
dc.relation.ispartofJournal of Biomedical Materials Research-
dc.titleComplement-mediated leukocyte adhesion on poly(etherurethane ureas) under shear stress in vitro-
dc.typeArticle-
dc.description.natureLink_to_subscribed_fulltext-
dc.identifier.doi10.1002/(SICI)1097-4636(199609)32:1<99::AID-JBM12>3.0.CO;2-D-
dc.identifier.pmid8864878-
dc.identifier.scopuseid_2-s2.0-0030246967-
dc.identifier.volume32-
dc.identifier.issue1-
dc.identifier.spage99-
dc.identifier.epage109-

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