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Book Chapter: Isolation of chloroplasts from plant protoplasts

TitleIsolation of chloroplasts from plant protoplasts
Authors
Issue Date2014
PublisherCold Spring Harbor Laboratory Press
Citation
Isolation of chloroplasts from plant protoplasts. In Paul R. Pryor (Ed.), Subcellular fractionation: a laboratory manual, p. 159-163. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press, 2014 How to Cite?
AbstractChloroplasts can be isolated from higher plants directly following homogenization; however, the resulting yield, purity, and intactness are often low, necessitating a large amount of starting materials. This protocol is optimized to produce a high yield of pure chloroplasts from isolated Arabidopsis protoplasts. The two-part method is a simple, scaled-down, and low-cost procedure that readily provides healthy mesophyll protoplasts, which are then ruptured to release intact chloroplasts. Chloroplasts isolated using this method are competent for use in biochemical, cellular, and molecular analyses.
Persistent Identifierhttp://hdl.handle.net/10722/215941
ISBN

 

DC FieldValueLanguage
dc.contributor.authorLung, SC-
dc.contributor.authorSmith, MD-
dc.contributor.authorChuong, SDX-
dc.date.accessioned2015-08-21T13:45:17Z-
dc.date.available2015-08-21T13:45:17Z-
dc.date.issued2014-
dc.identifier.citationIsolation of chloroplasts from plant protoplasts. In Paul R. Pryor (Ed.), Subcellular fractionation: a laboratory manual, p. 159-163. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press, 2014-
dc.identifier.isbn9781621820420-
dc.identifier.urihttp://hdl.handle.net/10722/215941-
dc.description.abstractChloroplasts can be isolated from higher plants directly following homogenization; however, the resulting yield, purity, and intactness are often low, necessitating a large amount of starting materials. This protocol is optimized to produce a high yield of pure chloroplasts from isolated Arabidopsis protoplasts. The two-part method is a simple, scaled-down, and low-cost procedure that readily provides healthy mesophyll protoplasts, which are then ruptured to release intact chloroplasts. Chloroplasts isolated using this method are competent for use in biochemical, cellular, and molecular analyses.-
dc.languageeng-
dc.publisherCold Spring Harbor Laboratory Press-
dc.relation.ispartofSubcellular fractionation: a laboratory manual-
dc.titleIsolation of chloroplasts from plant protoplasts-
dc.typeBook_Chapter-
dc.identifier.emailLung, SC: sclung@hku.hk-
dc.identifier.doi10.1101/pdb.prot074559-
dc.identifier.hkuros246396-
dc.identifier.spage159-
dc.identifier.epage163-
dc.publisher.placeCold Spring Harbor, New York-

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