File Download
  Links for fulltext
     (May Require Subscription)
  • Find via Find It@HKUL
Supplementary

Conference Paper: Increased synthesis of CD44s and its variants in kidney fibrosis of lupus nephritis

TitleIncreased synthesis of CD44s and its variants in kidney fibrosis of lupus nephritis
Authors
Issue Date2014
PublisherAmerican Society of Nephrology. The abstract suppl.'s website is located at https://www.asn-online.org/abstracts/
Citation
The 2014 Annual Meeting and Scientific Exposition of the American Society of Nephrology (Kidney Week 2014), Philadelphia, PA., 11-16 November 2014. In Journal of the American Society of Nephrology Abstract Supplement, 2014, p. 486A-487A, abstract no. FR-PO510 How to Cite?
AbstractBACKGROUND: Lupus nephritis is characterized by immune-mediated renal injury leading to glomerular and tubulo-interstitial fibrosis. CD44 is the major cell surface receptor for hyaluronan (HA) which has been implicated in inflammatory and fibrotic processes. During pathological conditions, splice variants of CD44 may also be induced. We investigated renal expression of standard CD44 (CD44s) and its variants in patients and mice with lupus nephritis, and their role in renal fibrosis. METHODS: CD44s, CD44v3 and CD44v6 expression was determined in kidney biopsies from patients with diffuse proliferative lupus nephritis and normal kidney tissue using cytochemical staining. Expression of CD44s, CD44v3 and CD44v6 was examined in NZB/W mice during disease progression. Mesangial cells were isolated from the renal cortex of NZB/W mice to investigate the mechanisms of CD44 synthesis. RESULTS: There was intense staining for CD44s and its variants in the glomeruli and renal tubules in kidney biopsies from patients with lupus nephritis, compared with weak expression in controls. CD44v3, but not CD44s and CD44v6, was also noted in cells infiltrating the glomerulus, peri-glomerular area, and interstitium. Compared to mice before the development of nephritis, murine renal cortical CD44s gene expression was significantly higher during active nephritis (proteinuria >3g/l), and continued to increase during progressive glomerular and tubulo-interstitial fibrosis (P<0.01 and P<0.001 respectively). CD44s, CD44v3 and CD44v6 expression in NZB/W mice was associated with glomerular and tubulo-interstitial fibrosis. Glomerular CD44s co-localized with collagen deposition, while tubulo-interstitial CD44s expression was associated with inflammatory cell infiltration, tubular atrophy and tubulo-interstitial fibrosis. CD44s, CD44v3 and CD44v6 were constitutive expressed in mesangial cells from NZB/W mice. Stimulation of mesangial cells with HA, IL-6, IL-1b, TNF-a, but not IFN-g, increased CD44s and CD44v3 but not CD44v6 synthesis. CONCLUSIONS: Our data suggested a role of CD44s and its variants in renal fibrosis due to lupus nephritis, and the modulatory effect of pro-inflammatory cytokines on their expression.
DescriptionFriday Poster: FR-PO510
Persistent Identifierhttp://hdl.handle.net/10722/214858
ISSN
2015 Impact Factor: 8.491
2015 SCImago Journal Rankings: 4.699

 

DC FieldValueLanguage
dc.contributor.authorChan, DTM-
dc.contributor.authorTse, WW-
dc.contributor.authorChau, MKM-
dc.contributor.authorYung, S-
dc.date.accessioned2015-08-21T11:58:59Z-
dc.date.available2015-08-21T11:58:59Z-
dc.date.issued2014-
dc.identifier.citationThe 2014 Annual Meeting and Scientific Exposition of the American Society of Nephrology (Kidney Week 2014), Philadelphia, PA., 11-16 November 2014. In Journal of the American Society of Nephrology Abstract Supplement, 2014, p. 486A-487A, abstract no. FR-PO510-
dc.identifier.issn1046-6673-
dc.identifier.urihttp://hdl.handle.net/10722/214858-
dc.descriptionFriday Poster: FR-PO510-
dc.description.abstractBACKGROUND: Lupus nephritis is characterized by immune-mediated renal injury leading to glomerular and tubulo-interstitial fibrosis. CD44 is the major cell surface receptor for hyaluronan (HA) which has been implicated in inflammatory and fibrotic processes. During pathological conditions, splice variants of CD44 may also be induced. We investigated renal expression of standard CD44 (CD44s) and its variants in patients and mice with lupus nephritis, and their role in renal fibrosis. METHODS: CD44s, CD44v3 and CD44v6 expression was determined in kidney biopsies from patients with diffuse proliferative lupus nephritis and normal kidney tissue using cytochemical staining. Expression of CD44s, CD44v3 and CD44v6 was examined in NZB/W mice during disease progression. Mesangial cells were isolated from the renal cortex of NZB/W mice to investigate the mechanisms of CD44 synthesis. RESULTS: There was intense staining for CD44s and its variants in the glomeruli and renal tubules in kidney biopsies from patients with lupus nephritis, compared with weak expression in controls. CD44v3, but not CD44s and CD44v6, was also noted in cells infiltrating the glomerulus, peri-glomerular area, and interstitium. Compared to mice before the development of nephritis, murine renal cortical CD44s gene expression was significantly higher during active nephritis (proteinuria >3g/l), and continued to increase during progressive glomerular and tubulo-interstitial fibrosis (P<0.01 and P<0.001 respectively). CD44s, CD44v3 and CD44v6 expression in NZB/W mice was associated with glomerular and tubulo-interstitial fibrosis. Glomerular CD44s co-localized with collagen deposition, while tubulo-interstitial CD44s expression was associated with inflammatory cell infiltration, tubular atrophy and tubulo-interstitial fibrosis. CD44s, CD44v3 and CD44v6 were constitutive expressed in mesangial cells from NZB/W mice. Stimulation of mesangial cells with HA, IL-6, IL-1b, TNF-a, but not IFN-g, increased CD44s and CD44v3 but not CD44v6 synthesis. CONCLUSIONS: Our data suggested a role of CD44s and its variants in renal fibrosis due to lupus nephritis, and the modulatory effect of pro-inflammatory cytokines on their expression.-
dc.languageeng-
dc.publisherAmerican Society of Nephrology. The abstract suppl.'s website is located at https://www.asn-online.org/abstracts/-
dc.relation.ispartofJournal of the American Society of Nephrology Abstract Supplement-
dc.titleIncreased synthesis of CD44s and its variants in kidney fibrosis of lupus nephritis-
dc.typeConference_Paper-
dc.identifier.emailChan, DTM: dtmchan@hkucc.hku.hk-
dc.identifier.emailTse, WW: kenniskt@hku.hk-
dc.identifier.emailChau, MKM: melchau@hku.hk-
dc.identifier.emailYung, S: ssyyung@hku.hk-
dc.identifier.authorityChan, DTM=rp00394-
dc.identifier.authorityYung, S=rp00455-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.hkuros247476-
dc.identifier.spage486A, abstract no. FR-PO510-
dc.identifier.epage487A-
dc.publisher.placeUnited States-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats