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Conference Paper: Differential response of human monocyte subsets to heterogeneous Pg-LPS Lipid-A structures

TitleDifferential response of human monocyte subsets to heterogeneous Pg-LPS Lipid-A structures
Authors
KeywordsPeriodontal disease
Monocytes
Lipopolysaccharide
Porphyromonas gingivalis
Inflammatory response
Issue Date2015
PublisherSage Publications, Inc.
Citation
The 2015 IADR/AADR/CADR General Session & Exhibition, Boston, MA., 11-14 March 2015. In Journal of Dental Research Meeting Abstracts, 2015, v. 94 Spec. Iss. A, abstract no. 4226 How to Cite?
AbstractOBJECTIVES: Porphyromonas gingivalis (Pg) is currently regarded as a key periodontal pathogen. Pg-lipopolysacharide (LPS) displays a remarkable heterogeneity with both tetra-(LPS1435/1449) and penta-acylated (LPS1690) lipid A structures. Pg-LPS heterogeneity accounts for differential immuno-inflammatory response and thereby contributes to periodontal pathogenesis. Monocytes are heterogeneous and can be classified into three different sub-populations. We recently reported the different pro-inflammatory response of total human monocytes to Pg-LPS with heterogeneous lipid-A structures. The present study further investigated the immuno-inflammatory response of three human monocyte subsets to different isoforms of Pg-LPS. METHODS: Three human monocyte subsets viz. CD14++CD16- (classical), CD14++CD16+ (intermediate) and CD14+CD16++ (non-classical) were isolated by FACS sorting. Purified monocyte subsets were then stimulated with 100 ng/ml of Pg-LPS1435 or Pg-LPS1690 for 2 to 24 h, and E. coli LPS served as a positive control. Gene and protein expression of immuno-inflammatory markers in cellular fraction and culture supernatant was analyzed with qPCR and ELISA, respectively. RESULTS: Monocyte subsets displayed markedly different immuno-inflammatory response to the heterogeneous Pg-LPS isoforms. Classical monocytes expressed highest levels of IL-6, IL-8, IL-1β and CCL-3, whereas intermediate monocytes expressed higher levels of IL-10 and GM-CSF in response to Pg-LPS1690 and E. coli LPS with reference to Pg-LPS1435. In contrast, TNF-α expression was the highest in non-classical monocytes. In addition, Pg-LPS1690 significantly up-regulated the expression of IL-6, IL-8 and TNF-α proteins in line with the gene expression profiles. CONCLUSIONS: This pioneering study demonstrates that human monocyte subsets may exhibit differential response to heterogeneous Pg-LPS isoforms, and contribute to the disruption of tissue homeostasis seen in periodontal disease, unravelling a new area of research in periodontology.Further study is required to determine the underlying molecular mechanisms and biological implications.
DescriptionePoster: abstract no. 4226
Persistent Identifierhttp://hdl.handle.net/10722/212176
ISSN
2015 Impact Factor: 4.602
2015 SCImago Journal Rankings: 1.714

 

DC FieldValueLanguage
dc.contributor.authorHerath Mudiyanselage, TDKH-
dc.contributor.authorGoh, B-
dc.contributor.authorSim, C-
dc.contributor.authorOng, S-
dc.contributor.authorSeneviratne, CJ-
dc.contributor.authorDarveau, RP-
dc.contributor.authorJin, L-
dc.contributor.authorWong, SSW-
dc.date.accessioned2015-07-21T02:26:20Z-
dc.date.available2015-07-21T02:26:20Z-
dc.date.issued2015-
dc.identifier.citationThe 2015 IADR/AADR/CADR General Session & Exhibition, Boston, MA., 11-14 March 2015. In Journal of Dental Research Meeting Abstracts, 2015, v. 94 Spec. Iss. A, abstract no. 4226-
dc.identifier.issn0022-0345-
dc.identifier.urihttp://hdl.handle.net/10722/212176-
dc.descriptionePoster: abstract no. 4226-
dc.description.abstractOBJECTIVES: Porphyromonas gingivalis (Pg) is currently regarded as a key periodontal pathogen. Pg-lipopolysacharide (LPS) displays a remarkable heterogeneity with both tetra-(LPS1435/1449) and penta-acylated (LPS1690) lipid A structures. Pg-LPS heterogeneity accounts for differential immuno-inflammatory response and thereby contributes to periodontal pathogenesis. Monocytes are heterogeneous and can be classified into three different sub-populations. We recently reported the different pro-inflammatory response of total human monocytes to Pg-LPS with heterogeneous lipid-A structures. The present study further investigated the immuno-inflammatory response of three human monocyte subsets to different isoforms of Pg-LPS. METHODS: Three human monocyte subsets viz. CD14++CD16- (classical), CD14++CD16+ (intermediate) and CD14+CD16++ (non-classical) were isolated by FACS sorting. Purified monocyte subsets were then stimulated with 100 ng/ml of Pg-LPS1435 or Pg-LPS1690 for 2 to 24 h, and E. coli LPS served as a positive control. Gene and protein expression of immuno-inflammatory markers in cellular fraction and culture supernatant was analyzed with qPCR and ELISA, respectively. RESULTS: Monocyte subsets displayed markedly different immuno-inflammatory response to the heterogeneous Pg-LPS isoforms. Classical monocytes expressed highest levels of IL-6, IL-8, IL-1β and CCL-3, whereas intermediate monocytes expressed higher levels of IL-10 and GM-CSF in response to Pg-LPS1690 and E. coli LPS with reference to Pg-LPS1435. In contrast, TNF-α expression was the highest in non-classical monocytes. In addition, Pg-LPS1690 significantly up-regulated the expression of IL-6, IL-8 and TNF-α proteins in line with the gene expression profiles. CONCLUSIONS: This pioneering study demonstrates that human monocyte subsets may exhibit differential response to heterogeneous Pg-LPS isoforms, and contribute to the disruption of tissue homeostasis seen in periodontal disease, unravelling a new area of research in periodontology.Further study is required to determine the underlying molecular mechanisms and biological implications.-
dc.languageeng-
dc.publisherSage Publications, Inc.-
dc.relation.ispartofJournal of Dental Research Meeting Abstracts-
dc.rightsJournal of Dental Research Meeting Abstracts. Copyright © Sage Publications, Inc.-
dc.subjectPeriodontal disease-
dc.subjectMonocytes-
dc.subjectLipopolysaccharide-
dc.subjectPorphyromonas gingivalis-
dc.subjectInflammatory response-
dc.titleDifferential response of human monocyte subsets to heterogeneous Pg-LPS Lipid-A structures-
dc.typeConference_Paper-
dc.identifier.emailHerath Mudiyanselage, TDKH: thanuja@hku.hk-
dc.identifier.emailSeneviratne, CJ: jaya@hku.hk-
dc.identifier.emailJin, L: ljjin@hkucc.hku.hk-
dc.identifier.emailWong, SSW: sarahsww@connect.hku.hk-
dc.identifier.authoritySeneviratne, CJ=rp01372-
dc.identifier.authorityJin, L=rp00028-
dc.identifier.hkuros245721-
dc.identifier.volume94-
dc.identifier.issueSpec. Iss. A-
dc.publisher.placeUnited States-

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