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Article: 17β-estradiol potentiates endothelium-dependent nitric oxide- and hyperpolarization-mediated relaxations in blood vessels of male but not female apolipoprotein-E deficient mice

Title17β-estradiol potentiates endothelium-dependent nitric oxide- and hyperpolarization-mediated relaxations in blood vessels of male but not female apolipoprotein-E deficient mice
Authors
Issue Date2015
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/vph
Citation
Vascular Pharmacology, 2015, v. 71, p. 166-173 How to Cite?
AbstractThe present study investigated the influence of gender on the changes underlying endothelial dysfunction in hyperlipidemia during aging. Isometric tension in rings (with endothelium) of the aortae and superior mesenteric arteries from apolipoprotein-E deficient mice was determined in wire myographs. Nitric oxide (NO)- and endothelium-dependent hyperpolarization (EDH)-mediated relaxations were smaller in the aortae and mesenteric arteries of 32weeks old males than eight weeks old males. In females, NO- and EDH-mediated relaxations were impaired only at 84weeks of age. The levels of reactive oxygen species were elevated in the blood vessels of 32weeks old males, but not females. Acute in vitro treatment with 17β-estradiol and apocynin improved NO- and EDH-mediated relaxations in 32weeks old males but not in 84weeks old males. Relaxations to SKA-31, activator of intermediate (IKCa) and small (SKCa) conductance calcium-activated potassium channels, were attenuated in the mesenteric arteries of 32weeks old males. Such impairment was restored by acute treatment with apocynin. These findings suggest that male hyperlipidemic mice develop endothelial dysfunction at an earlier age than females. This endothelial dysfunction is associated with impaired NO bioavailability and reduced IKCa and SKCa activity. Apocynin and 17β-estradiol restore the endothelial function only in younger male animals but not in older male or female animals. © 2015 Elsevier Inc..
Persistent Identifierhttp://hdl.handle.net/10722/211893
ISSN
2015 Impact Factor: 2.5
2015 SCImago Journal Rankings: 1.204

 

DC FieldValueLanguage
dc.contributor.authorKong, BWC-
dc.contributor.authorVanhoutte, PMGR-
dc.contributor.authorMan, RYK-
dc.contributor.authorLeung, SWS-
dc.date.accessioned2015-07-21T02:15:12Z-
dc.date.available2015-07-21T02:15:12Z-
dc.date.issued2015-
dc.identifier.citationVascular Pharmacology, 2015, v. 71, p. 166-173-
dc.identifier.issn1537-1891-
dc.identifier.urihttp://hdl.handle.net/10722/211893-
dc.description.abstractThe present study investigated the influence of gender on the changes underlying endothelial dysfunction in hyperlipidemia during aging. Isometric tension in rings (with endothelium) of the aortae and superior mesenteric arteries from apolipoprotein-E deficient mice was determined in wire myographs. Nitric oxide (NO)- and endothelium-dependent hyperpolarization (EDH)-mediated relaxations were smaller in the aortae and mesenteric arteries of 32weeks old males than eight weeks old males. In females, NO- and EDH-mediated relaxations were impaired only at 84weeks of age. The levels of reactive oxygen species were elevated in the blood vessels of 32weeks old males, but not females. Acute in vitro treatment with 17β-estradiol and apocynin improved NO- and EDH-mediated relaxations in 32weeks old males but not in 84weeks old males. Relaxations to SKA-31, activator of intermediate (IKCa) and small (SKCa) conductance calcium-activated potassium channels, were attenuated in the mesenteric arteries of 32weeks old males. Such impairment was restored by acute treatment with apocynin. These findings suggest that male hyperlipidemic mice develop endothelial dysfunction at an earlier age than females. This endothelial dysfunction is associated with impaired NO bioavailability and reduced IKCa and SKCa activity. Apocynin and 17β-estradiol restore the endothelial function only in younger male animals but not in older male or female animals. © 2015 Elsevier Inc..-
dc.languageeng-
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/vph-
dc.relation.ispartofVascular Pharmacology-
dc.rightsPosting accepted manuscript (postprint): © <year>. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/-
dc.title17β-estradiol potentiates endothelium-dependent nitric oxide- and hyperpolarization-mediated relaxations in blood vessels of male but not female apolipoprotein-E deficient mice-
dc.typeArticle-
dc.identifier.emailVanhoutte, PMGR: vanhoutt@hku.hk-
dc.identifier.emailMan, RYK: rykman@hku.hk-
dc.identifier.emailLeung, SWS: swsleung@hku.hk-
dc.identifier.authorityVanhoutte, PMGR=rp00238-
dc.identifier.authorityMan, RYK=rp00236-
dc.identifier.authorityLeung, SWS=rp00235-
dc.identifier.doi10.1016/j.vph.2015.02.009-
dc.identifier.pmid25869512-
dc.identifier.scopuseid_2-s2.0-84937969132-
dc.identifier.hkuros244683-
dc.identifier.volume71-
dc.identifier.spage166-
dc.identifier.epage173-
dc.publisher.placeUnited States-

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