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Article: Morphological and proteomic analyses of the biofilms generated by streptococcus mutans isolated from caries-active and caries-free adults

TitleMorphological and proteomic analyses of the biofilms generated by streptococcus mutans isolated from caries-active and caries-free adults
Authors
Issue Date2015
PublisherElsevier Ltd. The Journal's web site is located at http://www.e-jds.com
Citation
Journal of Dental Sciences, 2015, v. 10 n. 2, p. 206-215 How to Cite?
AbstractBackground/purpose: Biofilm formation by Streptococcus mutans is a prerequisite for the development of caries. Different strains of S. mutans may differ in their capacity in biofilm formation and protein expression. The objective of this study was to investigate the morphological features and proteomes of biofilms of S. mutans clinical isolates. Materials and methods: Clinical strains isolated from caries-active (SM 593) and caries-free (SM 18) adults were cultured on polystyrene sheets in tryptoneepolypeptoneeyeast extract medium. Biofilm formation and structure were assessed by confocal laser scanning microscopy and scanning electron microscopy. Proteins were extracted from SM 593 and SM 18 presented in biofilms and separated with two-dimensional gel electrophoresis, followed by peptide mass fingerprinting using matrix-assisted laser desorption time-of-flight mass spectrometry analysis. Results: Initially detected 2 hours after incubation, biofilm formation reached its maximum level at 20 hours. The biofilm formed by SM 593 was thicker with a higher percentage of viable bacteria compared with that formed by SM 18. Hydrolase and pantothenate kinase were detected in the SM 593 biofilm only, whereas 6-pyruvoyl tetrahydropterin synthase and phosphoribosylglycinamide formyltransferase were expressed exclusively in the SM 18 biofilm. Expressions of D-alanyl-D-alanine carboxypeptidase and response regulator homolog of RumR were most greatly enhanced in the SM 593 and SM 18 biofilms, respectively.
Persistent Identifierhttp://hdl.handle.net/10722/211676
ISSN
2015 Impact Factor: 0.795
2015 SCImago Journal Rankings: 0.242

 

DC FieldValueLanguage
dc.contributor.authorJiang, S-
dc.contributor.authorHuang, X-
dc.contributor.authorZhang, C-
dc.contributor.authorCai, Z-
dc.contributor.authorZou, T-
dc.date.accessioned2015-07-21T02:07:16Z-
dc.date.available2015-07-21T02:07:16Z-
dc.date.issued2015-
dc.identifier.citationJournal of Dental Sciences, 2015, v. 10 n. 2, p. 206-215-
dc.identifier.issn1991-7902-
dc.identifier.urihttp://hdl.handle.net/10722/211676-
dc.description.abstractBackground/purpose: Biofilm formation by Streptococcus mutans is a prerequisite for the development of caries. Different strains of S. mutans may differ in their capacity in biofilm formation and protein expression. The objective of this study was to investigate the morphological features and proteomes of biofilms of S. mutans clinical isolates. Materials and methods: Clinical strains isolated from caries-active (SM 593) and caries-free (SM 18) adults were cultured on polystyrene sheets in tryptoneepolypeptoneeyeast extract medium. Biofilm formation and structure were assessed by confocal laser scanning microscopy and scanning electron microscopy. Proteins were extracted from SM 593 and SM 18 presented in biofilms and separated with two-dimensional gel electrophoresis, followed by peptide mass fingerprinting using matrix-assisted laser desorption time-of-flight mass spectrometry analysis. Results: Initially detected 2 hours after incubation, biofilm formation reached its maximum level at 20 hours. The biofilm formed by SM 593 was thicker with a higher percentage of viable bacteria compared with that formed by SM 18. Hydrolase and pantothenate kinase were detected in the SM 593 biofilm only, whereas 6-pyruvoyl tetrahydropterin synthase and phosphoribosylglycinamide formyltransferase were expressed exclusively in the SM 18 biofilm. Expressions of D-alanyl-D-alanine carboxypeptidase and response regulator homolog of RumR were most greatly enhanced in the SM 593 and SM 18 biofilms, respectively.-
dc.languageeng-
dc.publisherElsevier Ltd. The Journal's web site is located at http://www.e-jds.com-
dc.relation.ispartofJournal of Dental Sciences-
dc.titleMorphological and proteomic analyses of the biofilms generated by streptococcus mutans isolated from caries-active and caries-free adults-
dc.typeArticle-
dc.identifier.emailZhang, C: zhangcf@hku.hk-
dc.identifier.authorityZhang, C=rp01408-
dc.identifier.doi10.1016/j.jds.2014.09.001-
dc.identifier.hkuros244441-
dc.identifier.volume10-
dc.identifier.issue2-
dc.identifier.spage206-
dc.identifier.epage215-
dc.publisher.placeUnited Kingdom-

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