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Article: Accurate measurement of stiffness of leukemia cells and leukocytes using an optical trap by rate-jump method

TitleAccurate measurement of stiffness of leukemia cells and leukocytes using an optical trap by rate-jump method
Authors
Issue Date2014
PublisherRSC Publications. The Journal's web site is located at http://pubs.rsc.org/en/journals/journalissues/ra#!recentarticles&all
Citation
RSC Advances, 2014, v. 4 n. 17, p. 8453-8460 How to Cite?
AbstractAccurate measurement of the elastic modulus of soft biological cells in the micro/nano scale range is still a challenging task. Tests involving constant-rate loading often yield results that are rate dependent, due to the viscous component of the deformation. In this work, a rate-jump indentation method was employed in an optical tweezers system to measure the stiffness of non-adherent blood cells, which are the softest types of cells. Compared to the traditional Hertzian method of indentation, the rate-jump method is found to be able to yield invariant elastic modulus from K562 myelogenous leukemia cells. The optical tweezers indentation method proposed can therefore serve as a standard protocol for obtaining the intrinsic elastic modulus of extremely soft cells, with applied forces in the pico-newton range. This method is also found to be effective in grading the stiffness values of myelogenous leukemia cell lines (K562 and HL60) and normal leukocytes, indicating that it can be used to identify normal cells from diseased counterparts without biochemical analysis.
Persistent Identifierhttp://hdl.handle.net/10722/211503
ISSN
2015 Impact Factor: 3.289
2015 SCImago Journal Rankings: 0.990

 

DC FieldValueLanguage
dc.contributor.authorZhou, ZL-
dc.contributor.authorHui, TH-
dc.contributor.authorTang, B-
dc.contributor.authorNgan, AHW-
dc.date.accessioned2015-07-16T01:27:31Z-
dc.date.available2015-07-16T01:27:31Z-
dc.date.issued2014-
dc.identifier.citationRSC Advances, 2014, v. 4 n. 17, p. 8453-8460-
dc.identifier.issn2046-2069-
dc.identifier.urihttp://hdl.handle.net/10722/211503-
dc.description.abstractAccurate measurement of the elastic modulus of soft biological cells in the micro/nano scale range is still a challenging task. Tests involving constant-rate loading often yield results that are rate dependent, due to the viscous component of the deformation. In this work, a rate-jump indentation method was employed in an optical tweezers system to measure the stiffness of non-adherent blood cells, which are the softest types of cells. Compared to the traditional Hertzian method of indentation, the rate-jump method is found to be able to yield invariant elastic modulus from K562 myelogenous leukemia cells. The optical tweezers indentation method proposed can therefore serve as a standard protocol for obtaining the intrinsic elastic modulus of extremely soft cells, with applied forces in the pico-newton range. This method is also found to be effective in grading the stiffness values of myelogenous leukemia cell lines (K562 and HL60) and normal leukocytes, indicating that it can be used to identify normal cells from diseased counterparts without biochemical analysis.-
dc.languageeng-
dc.publisherRSC Publications. The Journal's web site is located at http://pubs.rsc.org/en/journals/journalissues/ra#!recentarticles&all-
dc.relation.ispartofRSC Advances-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.titleAccurate measurement of stiffness of leukemia cells and leukocytes using an optical trap by rate-jump method-
dc.typeArticle-
dc.identifier.emailZhou, ZL: zlzhou@hku.hk-
dc.identifier.emailNgan, AHW: hwngan@hku.hk-
dc.identifier.authorityNgan, AHW=rp00225-
dc.description.naturepostprint-
dc.identifier.doi10.1039/c3ra45835k-
dc.identifier.hkuros244353-
dc.identifier.volume4-
dc.identifier.issue17-
dc.identifier.spage8453-
dc.identifier.epage8460-
dc.publisher.placeUnited Kingdom-

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