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Article: Systems-level quantification of division timing reveals a common genetic architecture controlling asynchrony and fate asymmetry

TitleSystems-level quantification of division timing reveals a common genetic architecture controlling asynchrony and fate asymmetry
Authors
KeywordsC. elegans
asynchrony of cell division
automated lineaging
cell cycle length
cell division timing
Issue Date2015
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/msb/index.html
Citation
Molecular Systems Biology, 2015, v. 11 n. 6, p. 814 How to Cite?
AbstractCoordination of cell division timing is crucial for proper cell fate specification and tissue growth. However, the differential regulation of cell division timing across or within cell types during metazoan development remains poorly understood. To elucidate the systems-level genetic architecture coordinating division timing, we performed a high-content screening for genes whose depletion produced a significant reduction in the asynchrony of division between sister cells (ADS) compared to that of wild-type during Caenorhabditis elegans embryogenesis. We quantified division timing using 3D time-lapse imaging followed by computer-aided lineage analysis. A total of 822 genes were selected for perturbation based on their conservation and known roles in development. Surprisingly, we find that cell fate determinants are not only essential for establishing fate asymmetry, but also are imperative for setting the ADS regardless of cellular context, indicating a common genetic architecture used by both cellular processes. The fate determinants demonstrate either coupled or separate regulation between the two processes. The temporal coordination appears to facilitate cell migration during fate specification or tissue growth. Our quantitative dataset with cellular resolution provides a resource for future analyses of the genetic control of spatial and temporal coordination during metazoan development.
Persistent Identifierhttp://hdl.handle.net/10722/210597
ISSN
2015 Impact Factor: 10.581
2015 SCImago Journal Rankings: 8.870
PubMed Central ID

 

DC FieldValueLanguage
dc.contributor.authorHo, VW-
dc.contributor.authorWong, MK-
dc.contributor.authorAn, X-
dc.contributor.authorGuan, D-
dc.contributor.authorShao, J-
dc.contributor.authorNg, HC-
dc.contributor.authorRen, X-
dc.contributor.authorHe, K-
dc.contributor.authorLiao, J-
dc.contributor.authorAng, Y-
dc.contributor.authorChen, L-
dc.contributor.authorHuang, X-
dc.contributor.authorYan, B-
dc.contributor.authorXia, Y-
dc.contributor.authorChan, LL-
dc.contributor.authorChow, KL-
dc.contributor.authorYan, H-
dc.contributor.authorZhao, Z-
dc.date.accessioned2015-06-19T03:54:03Z-
dc.date.available2015-06-19T03:54:03Z-
dc.date.issued2015-
dc.identifier.citationMolecular Systems Biology, 2015, v. 11 n. 6, p. 814-
dc.identifier.issn1744-4292-
dc.identifier.urihttp://hdl.handle.net/10722/210597-
dc.description.abstractCoordination of cell division timing is crucial for proper cell fate specification and tissue growth. However, the differential regulation of cell division timing across or within cell types during metazoan development remains poorly understood. To elucidate the systems-level genetic architecture coordinating division timing, we performed a high-content screening for genes whose depletion produced a significant reduction in the asynchrony of division between sister cells (ADS) compared to that of wild-type during Caenorhabditis elegans embryogenesis. We quantified division timing using 3D time-lapse imaging followed by computer-aided lineage analysis. A total of 822 genes were selected for perturbation based on their conservation and known roles in development. Surprisingly, we find that cell fate determinants are not only essential for establishing fate asymmetry, but also are imperative for setting the ADS regardless of cellular context, indicating a common genetic architecture used by both cellular processes. The fate determinants demonstrate either coupled or separate regulation between the two processes. The temporal coordination appears to facilitate cell migration during fate specification or tissue growth. Our quantitative dataset with cellular resolution provides a resource for future analyses of the genetic control of spatial and temporal coordination during metazoan development.-
dc.languageeng-
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/msb/index.html-
dc.relation.ispartofMolecular Systems Biology-
dc.subjectC. elegans-
dc.subjectasynchrony of cell division-
dc.subjectautomated lineaging-
dc.subjectcell cycle length-
dc.subjectcell division timing-
dc.titleSystems-level quantification of division timing reveals a common genetic architecture controlling asynchrony and fate asymmetry-
dc.typeArticle-
dc.identifier.emailYan, B: yanbinai6017@gmail.com-
dc.identifier.authorityYan, B=rp01940-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.15252/msb.20145857-
dc.identifier.pmid26063786-
dc.identifier.pmcidPMC4501849-
dc.identifier.hkuros251627-
dc.identifier.volume11-
dc.identifier.issue6-
dc.identifier.spage814-
dc.identifier.epage814-
dc.publisher.placeUnited Kingdom-

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