granted patent: METHODS FOR NUCLEIC ACID MAPPING AND IDENTIFICATION OF FINE-STRUCTURAL-VARIATIONS IN NUCLEIC ACIDS

TitleMETHODS FOR NUCLEIC ACID MAPPING AND IDENTIFICATION OF FINE-STRUCTURAL-VARIATIONS IN NUCLEIC ACIDS
Priority Date2008-07-10 US 12/129660P
2008-12-01 US 12/193442P
Inventors
Issue Date2010
Citation
WO Patent WO 2010003316. World Intellectual Property Organization (WIPO), PatentScope, 2010 How to Cite?
AbstractA method of juxtaposing sequence tags (GVTs) that are unique positional markers along the length of a population of target nucleic acid molecules is provided, the method comprising: fragmenting the target nucleic acid molecule to form target DNA insert; ligating the target DNA insert to a DNA vector or backbone to create a circular molecule; digesting the target DNA insert endonuclease to cleave the target DNA insert at a distance from each end of the target DNA insert yielding two GVTs comprising terminal sequences of the target DNA insert attached to an undigested linear backbone; recircularizing the linear backbone with the attached GVTs to obtain a circular DNA containing a GVT-pair having two juxtaposed GVTs; and recovering the GVT-pair DNA by nucleic acid amplification or digestion with endonuclease having sites flanking the GVT-pair. Cosmid vectors are provided for creating GVT-pairs of -45- to 50-kb separation sequencable by next-generation DNA sequencers.
Persistent Identifierhttp://hdl.handle.net/10722/210561
References

 

DC FieldValueLanguage
dc.date.accessioned2015-06-17T07:04:52Z-
dc.date.available2015-06-17T07:04:52Z-
dc.date.issued2010-
dc.identifier.citationWO Patent WO 2010003316. World Intellectual Property Organization (WIPO), PatentScope, 2010-
dc.identifier.urihttp://hdl.handle.net/10722/210561-
dc.description.abstractA method of juxtaposing sequence tags (GVTs) that are unique positional markers along the length of a population of target nucleic acid molecules is provided, the method comprising: fragmenting the target nucleic acid molecule to form target DNA insert; ligating the target DNA insert to a DNA vector or backbone to create a circular molecule; digesting the target DNA insert endonuclease to cleave the target DNA insert at a distance from each end of the target DNA insert yielding two GVTs comprising terminal sequences of the target DNA insert attached to an undigested linear backbone; recircularizing the linear backbone with the attached GVTs to obtain a circular DNA containing a GVT-pair having two juxtaposed GVTs; and recovering the GVT-pair DNA by nucleic acid amplification or digestion with endonuclease having sites flanking the GVT-pair. Cosmid vectors are provided for creating GVT-pairs of -45- to 50-kb separation sequencable by next-generation DNA sequencers.-
dc.relation.isreferencedbyWO 2012028105 (A1) 2012-03-08-
dc.relation.isreferencedby C N101967684 (B) 2013-02-27-
dc.relation.isreferencedbyCN 101967684 (A) 2011-02-09 -
dc.relation.isreferencedbyWO 2014008635 (A1) 2014-01-16-
dc.relation.isreferencedbyWO 2012137197 (A2) 2012-10-11 -
dc.relation.isreferencedby W O2012137197 (A3) 2013-03-14-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License for public patent documents-
dc.titleMETHODS FOR NUCLEIC ACID MAPPING AND IDENTIFICATION OF FINE-STRUCTURAL-VARIATIONS IN NUCLEIC ACIDS-
dc.typePatent-
dc.identifier.authorityLOK, SI=rp00271-
dc.description.naturepublished_or_final_version-
dc.contributor.inventorLOK, SI-
patents.identifier.applicationPC T/CN2009000777-
patents.description.assigneeLOK SI [CN] -
patents.description.countryWorld Intellectual Property Organization (WIPO) -
patents.date.publication2010-01-14-
dc.relation.references W O2007087291 (A3) 2008-05-29-
dc.relation.referencesWO 2007076726 (A1) 2007-07-12-
dc.relation.referencesWO 2007145612 (A1) 2007-12-21 -
dc.relation.references W O2007145612 (A8) 2008-02-28-
dc.relation.referencesWO 2007087291 (A2) 2007-08-02 -
dc.relation.referencesWO 2005042781 (A2) 2005-05-12 -
dc.relation.references W O2005042781 (A8) 2005-08-11 -
dc.relation.references W O2005042781 (A3) 2005-11-03-
dc.relation.referencesWO 2008083554 (A1) 2008-07-17-
dc.relation.referencesXP 002523083-
patents.identifier.hkutechidGRC-2006-00241-7-
patents.date.application2009-07-09-
patents.date.priority2008-07-10 US 12/129660P-
patents.date.priority2008-12-01 US 12/193442P-
patents.description.ccwo-
patents.identifier.publicationWO 2010003316-
patents.relation.familyCN 101395281 (A) 2009-03-25 -
patents.relation.familyWO 2008083554 (A1) 2008-07-17-
patents.relation.familyEP 1969146 (A1) 2008-09-17 -
patents.relation.familyCN 102165073 (A) 2011-08-24-
patents.relation.familyAT 483804 (T) 2010-10-15-
patents.relation.familyEP 2310524 (A1) 2011-04-20 -
patents.relation.familyUS 7932029 (B1) 2011-04-26-
patents.relation.familyUS 2009325239 (A1) 2009-12-31 -
patents.relation.family U S8329400 (B2) 2012-12-11-
patents.relation.familyUS 2009156431 (A1) 2009-06-18-
patents.relation.familyWO 2007076726 (A1) 2007-07-12-
patents.description.kindA1-
patents.typePatent_granted-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats