granted patent: METHODS FOR NUCLEIC ACID MAPPING AND IDENTIFICATION OF FINE-STRUCTURAL-VARIATIONS IN NUCLEIC ACIDS

TitleMETHODS FOR NUCLEIC ACID MAPPING AND IDENTIFICATION OF FINE-STRUCTURAL-VARIATIONS IN NUCLEIC ACIDS
Granted PatentUS 20090325239
US 8329400
Granted Date2009-12-31
Priority Date2006-06-15 US 11/814378P
2006-04-17 US 11/792926P
2006-01-04 US 11/756417P
2008-12-01 US 12/193442P
2008-07-10 US 12/129660P
2007-01-03 US 11/649587
2007-12-12 US 11/954947
2009-07-10 US 12/501136
Inventors
Issue Date2009
Citation
US Patent US 20090325239. Washington, DC: US Patent and Trademark Office (USPTO), 2009 How to Cite?
AbstractA method of juxtaposing sequence tags (GVTs) that are unique positional markers along the length of a population of target nucleic acid molecules is provided, the method comprising: fragmenting the target nucleic acid molecule to form target DNA insert; ligating the target DNA insert to a DNA vector or backbone to create a circular molecule; digesting the target DNA insert endonuclease to cleave the target DNA insert at a distance from each end of the target DNA insert yielding two GVTs comprising terminal sequences of the target DNA insert attached to an undigested linear backbone; recircularizing the linear backbone with the attached GVTs to obtain a circular DNA containing a GVT-pair having two juxtaposed GVTs; and recovering the GVT-pair DNA by nucleic acid amplification or digestion with endonuclease having sites flanking the GVT-pair.; Cosmid vectors are provided for creating GVT-pairs of ~45- to 50-kb separation sequencable by next-generation DNA sequencers.
Persistent Identifierhttp://hdl.handle.net/10722/210542

 

DC FieldValueLanguage
dc.date.accessioned2015-06-17T07:04:16Z-
dc.date.available2015-06-17T07:04:16Z-
dc.date.issued2009-
dc.identifier.citationUS Patent US 20090325239. Washington, DC: US Patent and Trademark Office (USPTO), 2009-
dc.identifier.urihttp://hdl.handle.net/10722/210542-
dc.description.abstractA method of juxtaposing sequence tags (GVTs) that are unique positional markers along the length of a population of target nucleic acid molecules is provided, the method comprising: fragmenting the target nucleic acid molecule to form target DNA insert; ligating the target DNA insert to a DNA vector or backbone to create a circular molecule; digesting the target DNA insert endonuclease to cleave the target DNA insert at a distance from each end of the target DNA insert yielding two GVTs comprising terminal sequences of the target DNA insert attached to an undigested linear backbone; recircularizing the linear backbone with the attached GVTs to obtain a circular DNA containing a GVT-pair having two juxtaposed GVTs; and recovering the GVT-pair DNA by nucleic acid amplification or digestion with endonuclease having sites flanking the GVT-pair.; Cosmid vectors are provided for creating GVT-pairs of ~45- to 50-kb separation sequencable by next-generation DNA sequencers.-
dc.relation.isreferencedbyCN 102559856 (A) 2012-07-11 -
dc.relation.isreferencedbyWO 2012083845 (A1) 2012-06-28-
dc.relation.isreferencedbyUS 2011225038 (A1) 2011-09-15-
dc.relation.isreferencedby C N102559856 (B) 2014-03-12-
dc.relation.isreferencedbyUS 7932029 (B1) 2011-04-26-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License for public patent documents-
dc.titleMETHODS FOR NUCLEIC ACID MAPPING AND IDENTIFICATION OF FINE-STRUCTURAL-VARIATIONS IN NUCLEIC ACIDS-
dc.typePatent-
dc.identifier.authorityLOK SI=rp00271-
dc.description.naturepublished_or_final_version-
dc.contributor.inventorLOK SI-
patents.identifier.applicationUS 12/501136-
patents.identifier.grantedUS 20090325239-
patents.identifier.grantedUS 8329400-
patents.description.assigneeLOK SI, ; VERSITECH LIMITED-
patents.description.countryUnited States of America -
patents.date.granted2009-12-31-
patents.identifier.hkutechidGRC-2006-00241-2-
patents.date.application2009-07-10-
patents.date.priority2006-06-15 US 11/814378P-
patents.date.priority2006-04-17 US 11/792926P-
patents.date.priority2006-01-04 US 11/756417P-
patents.date.priority2008-12-01 US 12/193442P-
patents.date.priority2008-07-10 US 12/129660P-
patents.date.priority2007-01-03 US 11/649587-
patents.date.priority2007-12-12 US 11/954947-
patents.date.priority2009-07-10 US 12/501136-
patents.description.ccus-
patents.relation.familyCN 101395281 (A) 2009-03-25 -
patents.relation.familyCN 102165073 (A) 2011-08-24-
patents.relation.familyEP 1969146 (A1) 2008-09-17 -
patents.relation.familyEP 2310524 (A1) 2011-04-20 -
patents.relation.familyUS 7932029 (B1) 2011-04-26-
patents.relation.familyUS 2009156431 (A1) 2009-06-18-
patents.relation.familyWO 2007076726 (A1) 2007-07-12-
patents.relation.familyWO 2008083554 (A1) 2008-07-17-
patents.relation.familyWO 2010003316 (A1) 2010-01-14-
patents.relation.familyAT 483804 (T) 2010-10-15-
patents.relation.family U S8329400 (B2) 2012-12-11-
patents.description.kindA1-
patents.typePatent_granted-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats