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Article: Adeno-associated virus mediated expression of human erythropoietin in vitro

TitleAdeno-associated virus mediated expression of human erythropoietin in vitro
Authors
Issue Date2002
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/aps/index.html
Citation
Acta Pharmacologica Sinica, 2002, v. 23 n. 1, p. 55-58 How to Cite?
AbstractAIM: To investigate the expression of human erythropoietin (hEpo) in 293 cell line using adeno-associated virus (AAV) vector, and provide information for its potential application in human gene therapy. METHODS: The human Epo cDNA was inserted into the AAV cassettes, and recombinant adeno-associated virus (rAAV-EPO) vectors were generated by cotransfection methods without helper virus. The expression of hEpo was detected by RT-PCR and ELISA methods. RESULTS: A recombinant adeno-associated virus expressing hEpo was constructed and generated successfully. Positive signal of mRNA was detected from 293 cells transduced with rAAV-EPO, and the hEpo level in supernatant reached 500 U/L. CONCLUSION: rAAV-EPO expressed biologically active hEpo effectively, suggesting that it is a suitable gene expression tool for further study in vivo experiments.
Persistent Identifierhttp://hdl.handle.net/10722/210022
ISSN
2015 Impact Factor: 3.166
2015 SCImago Journal Rankings: 1.161

 

DC FieldValueLanguage
dc.contributor.authorDiao, Y-
dc.contributor.authorXu, RA-
dc.contributor.authorWang, GJ-
dc.contributor.authorZhao, XC-
dc.date.accessioned2015-05-20T02:55:13Z-
dc.date.available2015-05-20T02:55:13Z-
dc.date.issued2002-
dc.identifier.citationActa Pharmacologica Sinica, 2002, v. 23 n. 1, p. 55-58-
dc.identifier.issn1671-4083-
dc.identifier.urihttp://hdl.handle.net/10722/210022-
dc.description.abstractAIM: To investigate the expression of human erythropoietin (hEpo) in 293 cell line using adeno-associated virus (AAV) vector, and provide information for its potential application in human gene therapy. METHODS: The human Epo cDNA was inserted into the AAV cassettes, and recombinant adeno-associated virus (rAAV-EPO) vectors were generated by cotransfection methods without helper virus. The expression of hEpo was detected by RT-PCR and ELISA methods. RESULTS: A recombinant adeno-associated virus expressing hEpo was constructed and generated successfully. Positive signal of mRNA was detected from 293 cells transduced with rAAV-EPO, and the hEpo level in supernatant reached 500 U/L. CONCLUSION: rAAV-EPO expressed biologically active hEpo effectively, suggesting that it is a suitable gene expression tool for further study in vivo experiments.-
dc.languageeng-
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/aps/index.html-
dc.relation.ispartofActa Pharmacologica Sinica-
dc.subject.meshDNA, Complementary - genetics-
dc.subject.meshDNA, Recombinant - genetics-
dc.subject.meshDependovirus - genetics - metabolism-
dc.subject.meshErythropoietin - biosynthesis - genetics - metabolism-
dc.subject.meshKidney - cytology - metabolism-
dc.titleAdeno-associated virus mediated expression of human erythropoietin in vitro-
dc.typeArticle-
dc.identifier.emailXu, RA: rxua@hkucc.hku.hk-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.pmid11860738-
dc.identifier.hkuros69127-
dc.identifier.volume23-
dc.identifier.issue1-
dc.identifier.spage55-
dc.identifier.epage58-
dc.publisher.placeUnited States-

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