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Conference Paper: Cytotoxicity evaluation of new resin matrix system on fiber-reinforced composites

TitleCytotoxicity evaluation of new resin matrix system on fiber-reinforced composites
Authors
KeywordsMedical sciences
Dentistry
Issue Date2013
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/dental
Citation
The 2013 Annual Meeting of the Academy of Dental Materials (ADM), Vancouver, BC., Canada, 9-12 October 2013. In Dental Materials, 2013, v. 29 suppl. 1, p. e61, abstract no. 124 How to Cite?
AbstractPURPOSE: Recent commercial resin matrix systems use bis-GMA as the basic matrix. A previous studies showed that bis-GMA affected the vitality of dental pulp and induced pulpal inflammation (Engelmann J, et al. Biomaterials 2004;25:4573), was able to disturb normal differentiation procedures of pulp fibroblasts (Imazato S, et al. J Biomed Mater Res Part B: Appl Biomater 2009;88B:378), and induced allergic contact stomatitis (Stoeva et al. J IMAB-Ann Proc 2008;book 2:45). A monomer alternative to bis-GMA, such as 1,6-hexanediol dimethacrylate (HDMA), is under current research. The purpose of this study was to determine the cytotoxicity property of HDMA on fibroblast cells by MTT method. METHODS AND MATERIALS: The materials used were bis-GMA (Sigma–Aldrich, USA), MMA (ProSciTech, Australia), HDMA (Esstech, USA), CQ (Esstech, USA), CEMA (Esstech, USA), Eglass fibers (Stick Tech, Finland), Vero fibroblast cell line (UGM, Indonesia). Fifteen specimens of FRCs (2×2×25)mm were prepared and divided into 3 groups. The experiment groups were 78.4%HDMA +19.6%MMA+ 1.0%CQ + 1.0%CEMA (Exp-1 group) and 49.0%HDMA +49.0%MMA+ 1.0%CQ + 1.0%CEMA Exp-2 group), whilst the control group was 78.4%bis-GMA+19.6%MMA+ 1.0%CQ + 1.0%CEMA. Specimens were milled and diluted in culture medium (0.1mg powder/1mL medium). Specimen solution of 100µL was added into 96-well plate containing fibroblast cells of 2×104 cells/100µL and incubated for 24 h. MTT of 10µL was added to the well, incubated for 4h, then 100µL stop solution was added. The OD of the cells viability was determined by a ELISA reader with a wavelength of 550 nm. Cells viability was calculated in percentage and analyzed by ANOVA and LSD. RESULTS: Analysis by one way ANOVA revealed significant differences of cells viability among the groups (p < 0.01). The LSD test showed a significant difference between exp-1 group and control group and also between exp-2 group and control group (p < 0.01), whilst no significant difference between exp-1 group and exp-2 group was found (p > 0.05). CONCLUSION: A resin matrix system based on HDMA–MMA (exp-1 group and exp-2 group) revealed a significant difference of fibroblast cells viability compared to a bis-GMA–MMA matrix system. The HDMA–MMA matrix systemwas less cytotoxic than bis-GMA–MMA matrix system.
DescriptionThis journal suppl. entitled: Abstracts of the Academy of Dental Materials Annual Meeting, 9-12 October 2013 - Vancouver, BC, Canada
Persistent Identifierhttp://hdl.handle.net/10722/209859
ISSN
2015 Impact Factor: 3.931
2015 SCImago Journal Rankings: 1.339

 

DC FieldValueLanguage
dc.contributor.authorSunarintyas, S-
dc.contributor.authorSiswomihardjo, W-
dc.contributor.authorSuwarsohartono, H-
dc.contributor.authorMatinlinna, JP-
dc.date.accessioned2015-05-18T03:28:28Z-
dc.date.available2015-05-18T03:28:28Z-
dc.date.issued2013-
dc.identifier.citationThe 2013 Annual Meeting of the Academy of Dental Materials (ADM), Vancouver, BC., Canada, 9-12 October 2013. In Dental Materials, 2013, v. 29 suppl. 1, p. e61, abstract no. 124-
dc.identifier.issn0109-5641-
dc.identifier.urihttp://hdl.handle.net/10722/209859-
dc.descriptionThis journal suppl. entitled: Abstracts of the Academy of Dental Materials Annual Meeting, 9-12 October 2013 - Vancouver, BC, Canada-
dc.description.abstractPURPOSE: Recent commercial resin matrix systems use bis-GMA as the basic matrix. A previous studies showed that bis-GMA affected the vitality of dental pulp and induced pulpal inflammation (Engelmann J, et al. Biomaterials 2004;25:4573), was able to disturb normal differentiation procedures of pulp fibroblasts (Imazato S, et al. J Biomed Mater Res Part B: Appl Biomater 2009;88B:378), and induced allergic contact stomatitis (Stoeva et al. J IMAB-Ann Proc 2008;book 2:45). A monomer alternative to bis-GMA, such as 1,6-hexanediol dimethacrylate (HDMA), is under current research. The purpose of this study was to determine the cytotoxicity property of HDMA on fibroblast cells by MTT method. METHODS AND MATERIALS: The materials used were bis-GMA (Sigma–Aldrich, USA), MMA (ProSciTech, Australia), HDMA (Esstech, USA), CQ (Esstech, USA), CEMA (Esstech, USA), Eglass fibers (Stick Tech, Finland), Vero fibroblast cell line (UGM, Indonesia). Fifteen specimens of FRCs (2×2×25)mm were prepared and divided into 3 groups. The experiment groups were 78.4%HDMA +19.6%MMA+ 1.0%CQ + 1.0%CEMA (Exp-1 group) and 49.0%HDMA +49.0%MMA+ 1.0%CQ + 1.0%CEMA Exp-2 group), whilst the control group was 78.4%bis-GMA+19.6%MMA+ 1.0%CQ + 1.0%CEMA. Specimens were milled and diluted in culture medium (0.1mg powder/1mL medium). Specimen solution of 100µL was added into 96-well plate containing fibroblast cells of 2×104 cells/100µL and incubated for 24 h. MTT of 10µL was added to the well, incubated for 4h, then 100µL stop solution was added. The OD of the cells viability was determined by a ELISA reader with a wavelength of 550 nm. Cells viability was calculated in percentage and analyzed by ANOVA and LSD. RESULTS: Analysis by one way ANOVA revealed significant differences of cells viability among the groups (p < 0.01). The LSD test showed a significant difference between exp-1 group and control group and also between exp-2 group and control group (p < 0.01), whilst no significant difference between exp-1 group and exp-2 group was found (p > 0.05). CONCLUSION: A resin matrix system based on HDMA–MMA (exp-1 group and exp-2 group) revealed a significant difference of fibroblast cells viability compared to a bis-GMA–MMA matrix system. The HDMA–MMA matrix systemwas less cytotoxic than bis-GMA–MMA matrix system.-
dc.languageeng-
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/dental-
dc.relation.ispartofDental Materials-
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Dental Materials. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Dental Materials, v. 29 suppl. 1, 2013. DOI: 10.1016/j.dental.2013.08.125-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectMedical sciences-
dc.subjectDentistry-
dc.titleCytotoxicity evaluation of new resin matrix system on fiber-reinforced composites-
dc.typeConference_Paper-
dc.identifier.emailMatinlinna, JP: jpmat@hku.hk-
dc.identifier.authorityMatinlinna, JP=rp00052-
dc.description.naturepostprint-
dc.identifier.doi10.1016/j.dental.2013.08.125-
dc.identifier.hkuros243149-
dc.identifier.volume29-
dc.identifier.issuesuppl. 1-
dc.identifier.spagee61, abstract no. 124-
dc.identifier.epagee61, abstract no. 124-
dc.publisher.placeUnited States-

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