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Article: The Arabidopsis cytosolic acyl-CoA-binding proteins play combinatory roles in pollen development

TitleThe Arabidopsis cytosolic acyl-CoA-binding proteins play combinatory roles in pollen development
Authors
Issue Date2015
PublisherOxford University Press. The Journal's web site is located at http://pcp.oxfordjournals.org/
Citation
Plant and Cell Physiology, 2015, v. 56 n. 2, p. 322-333 How to Cite?
AbstractIn Arabidopsis, six acyl-CoA-binding proteins (ACBPs) have been identified and they have been demonstrated to function in plant stress responses and development. Three of these AtACBPs (AtACBP4-AtACBP6) are cytosolic proteins and all are expressed in floral organs as well as in other tissues. The roles of cytosolic AtACBPs in floral development were addressed in this study. To this end, a T-DNA insertional knockout mutant of acbp5 was characterized before use in crosses with the already available acbp4 and acbp6 T-DNA knockout mutants to examine their independent and combinatory functions in floral development. The single-gene knockout mutations did not cause any significant phenotypic changes, while phenotypic deficiencies affecting siliques and pollen were observed in the double mutants (acbp4acbp6 and acbp5acbp6) and the acbp4acbp5acbp6 triple mutant. Vacuole accumulation in the acbp4acbp6, acbp5acbp6 and acbp4acbp5acbp6 pollen was the most severe abnormality occurring in the double and triple mutants. Furthermore, scanning electron microscopy and transmission electron microscopy revealed exine and oil body defects in the acbp4acbp5acbp6 mutant, which also displayed reduced ability in in vitro pollen germination. Transgenic Arabidopsis expressing β-glucuronidase (GUS) driven from the various AtACBP promoters indicated that AtACBP6pro::GUS expression overlapped with AtACBP4pro::GUS expression in pollen grains and with AtACBP5pro::GUS expression in the microspores and tapetal cells. Taken together, these results suggest that the three cytosolic AtACBPs play combinatory roles in acyl-lipid metabolism during pollen development. © The Author 2014. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/209367
ISSN
2015 Impact Factor: 4.319
2015 SCImago Journal Rankings: 2.301

 

DC FieldValueLanguage
dc.contributor.authorHsiao, ASen_US
dc.contributor.authorYeung, ECen_US
dc.contributor.authorYe, ZWen_US
dc.contributor.authorChye, MLen_US
dc.date.accessioned2015-04-17T05:11:24Z-
dc.date.available2015-04-17T05:11:24Z-
dc.date.issued2015en_US
dc.identifier.citationPlant and Cell Physiology, 2015, v. 56 n. 2, p. 322-333en_US
dc.identifier.issn0032-0781en_US
dc.identifier.urihttp://hdl.handle.net/10722/209367-
dc.description.abstractIn Arabidopsis, six acyl-CoA-binding proteins (ACBPs) have been identified and they have been demonstrated to function in plant stress responses and development. Three of these AtACBPs (AtACBP4-AtACBP6) are cytosolic proteins and all are expressed in floral organs as well as in other tissues. The roles of cytosolic AtACBPs in floral development were addressed in this study. To this end, a T-DNA insertional knockout mutant of acbp5 was characterized before use in crosses with the already available acbp4 and acbp6 T-DNA knockout mutants to examine their independent and combinatory functions in floral development. The single-gene knockout mutations did not cause any significant phenotypic changes, while phenotypic deficiencies affecting siliques and pollen were observed in the double mutants (acbp4acbp6 and acbp5acbp6) and the acbp4acbp5acbp6 triple mutant. Vacuole accumulation in the acbp4acbp6, acbp5acbp6 and acbp4acbp5acbp6 pollen was the most severe abnormality occurring in the double and triple mutants. Furthermore, scanning electron microscopy and transmission electron microscopy revealed exine and oil body defects in the acbp4acbp5acbp6 mutant, which also displayed reduced ability in in vitro pollen germination. Transgenic Arabidopsis expressing β-glucuronidase (GUS) driven from the various AtACBP promoters indicated that AtACBP6pro::GUS expression overlapped with AtACBP4pro::GUS expression in pollen grains and with AtACBP5pro::GUS expression in the microspores and tapetal cells. Taken together, these results suggest that the three cytosolic AtACBPs play combinatory roles in acyl-lipid metabolism during pollen development. © The Author 2014. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved.en_US
dc.languageengen_US
dc.publisherOxford University Press. The Journal's web site is located at http://pcp.oxfordjournals.org/en_US
dc.relation.ispartofPlant and Cell Physiologyen_US
dc.rightsPre-print: Journal Title] ©: [year] [owner as specified on the article] Published by Oxford University Press [on behalf of xxxxxx]. All rights reserved. Pre-print (Once an article is published, preprint notice should be amended to): This is an electronic version of an article published in [include the complete citation information for the final version of the Article as published in the print edition of the Journal.] Post-print: This is a pre-copy-editing, author-produced PDF of an article accepted for publication in [insert journal title] following peer review. The definitive publisher-authenticated version [insert complete citation information here] is available online at: xxxxxxx [insert URL that the author will receive upon publication here].en_US
dc.titleThe Arabidopsis cytosolic acyl-CoA-binding proteins play combinatory roles in pollen developmenten_US
dc.typeArticleen_US
dc.identifier.emailChye, ML: mlchye@hkucc.hku.hken_US
dc.identifier.authorityChye, ML=rp00687en_US
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1093/pcp/pcu163en_US
dc.identifier.pmid25395473en_US
dc.identifier.hkuros242763en_US
dc.identifier.volume56en_US
dc.identifier.issue2en_US
dc.identifier.spage322en_US
dc.identifier.epage333en_US
dc.publisher.placeUnited Kingdomen_US

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