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Conference Paper: Antimicrobial performance of five Hong Kong OTC chlorhexidine-based mouthrinse

TitleAntimicrobial performance of five Hong Kong OTC chlorhexidine-based mouthrinse
Authors
KeywordsChlorhexidine
Mouthrinse
OTC
Hong Kong
Antimicrobial effectiveness
Issue Date2015
PublisherSage Publications, Inc.
Citation
The 2015 IADR/AADR/CADR General Session & Exhibition, Boston, MA., 11-14 March 2015. In Journal of Dental Research Meeting Abstracts, 2015, v. 94 Spec. Iss. A, abstract no. 0295 How to Cite?
AbstractOBJECTIVES: Chlorhexidine is an active ingredient commonly available as mouthrinse. This in vitro study was to determine the antimicrobial activity against pathogenic oral bacteria and fungus between five Hong Kong OTC chlorhexidine-based mouthrinse using standard agar diffusion assay sensitivity test. METHODS: Oral Bacteria and fungus species, namely Streptococcus mutans, Streptococcus mitis, Streptococcus sanguinis, Streptococcus sobrinus, Lactobacillus acidophilus, Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans and Candida albicans were selected for the test. Five Hong Kong OTC 0.2% chlorhexidine glucontate mouthrinse manufactured by ECOLAB, GSK, VickmansPharm, EuroPharm and QualityPharmLab were randomly purchased and tested. All these mouthrinse were tested together before approximately 1 year before their expiration dates. Mouthrinse from GSK was treated as positive control and phosphate-buffered saline (PBS) was used as negative control. According to standard agar diffusion assay method, the cultures were identified, inoculated, harvested and suspended in PBS at a concentration of 1 × 106 cells/mL, equivalent to 0.5 MacFarland Standard Units. The mouthrinses and control materials were performed according to a standard and published protocol in triplicate. The mean diameter (mm) of growth inhibition of various areas in the test was measured by a calibrated ruler. Friedman non-parametric test and Dunn's post-hoc test were used to identify the statistical difference at α=0.05. RESULTS: The antimicrobial performance of the tested five chlorhexidine mouthrinses was ranked in descending order: QualityPharmLab, EuroPharm, VickmansPharm, ECOLAB, GSK. QualityPharmLab mouthrinse statistically (p<0.05) showed a superior antimicrobial performance than other mouthrinses except for Lactobacillus acidophilus, Porphyromonas gingivalis and Candida albicans. Other four mouthrinse demonstrated similar performance which was not statistically significant (p>0.05). CONCLUSIONS: Chlorhexidine-based mouthrinse is effective as an antimicrobial agent for oral use. A proper formulation and excipients are essential to sustain the antimicrobial effectiveness of chlorhexidine mouthrinse.
DescriptionOral Session - Chemical Plaque Control: abstract no. 0295
Persistent Identifierhttp://hdl.handle.net/10722/209355
ISSN
2015 Impact Factor: 4.602
2015 SCImago Journal Rankings: 1.714

 

DC FieldValueLanguage
dc.contributor.authorTsoi, JKH-
dc.contributor.authorCheong, CHL-
dc.contributor.authorWong, RWK-
dc.contributor.authorMcGrath, C-
dc.contributor.authorKing, N-
dc.date.accessioned2015-04-17T05:09:51Z-
dc.date.available2015-04-17T05:09:51Z-
dc.date.issued2015-
dc.identifier.citationThe 2015 IADR/AADR/CADR General Session & Exhibition, Boston, MA., 11-14 March 2015. In Journal of Dental Research Meeting Abstracts, 2015, v. 94 Spec. Iss. A, abstract no. 0295-
dc.identifier.issn0022-0345-
dc.identifier.urihttp://hdl.handle.net/10722/209355-
dc.descriptionOral Session - Chemical Plaque Control: abstract no. 0295-
dc.description.abstractOBJECTIVES: Chlorhexidine is an active ingredient commonly available as mouthrinse. This in vitro study was to determine the antimicrobial activity against pathogenic oral bacteria and fungus between five Hong Kong OTC chlorhexidine-based mouthrinse using standard agar diffusion assay sensitivity test. METHODS: Oral Bacteria and fungus species, namely Streptococcus mutans, Streptococcus mitis, Streptococcus sanguinis, Streptococcus sobrinus, Lactobacillus acidophilus, Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans and Candida albicans were selected for the test. Five Hong Kong OTC 0.2% chlorhexidine glucontate mouthrinse manufactured by ECOLAB, GSK, VickmansPharm, EuroPharm and QualityPharmLab were randomly purchased and tested. All these mouthrinse were tested together before approximately 1 year before their expiration dates. Mouthrinse from GSK was treated as positive control and phosphate-buffered saline (PBS) was used as negative control. According to standard agar diffusion assay method, the cultures were identified, inoculated, harvested and suspended in PBS at a concentration of 1 × 106 cells/mL, equivalent to 0.5 MacFarland Standard Units. The mouthrinses and control materials were performed according to a standard and published protocol in triplicate. The mean diameter (mm) of growth inhibition of various areas in the test was measured by a calibrated ruler. Friedman non-parametric test and Dunn's post-hoc test were used to identify the statistical difference at α=0.05. RESULTS: The antimicrobial performance of the tested five chlorhexidine mouthrinses was ranked in descending order: QualityPharmLab, EuroPharm, VickmansPharm, ECOLAB, GSK. QualityPharmLab mouthrinse statistically (p<0.05) showed a superior antimicrobial performance than other mouthrinses except for Lactobacillus acidophilus, Porphyromonas gingivalis and Candida albicans. Other four mouthrinse demonstrated similar performance which was not statistically significant (p>0.05). CONCLUSIONS: Chlorhexidine-based mouthrinse is effective as an antimicrobial agent for oral use. A proper formulation and excipients are essential to sustain the antimicrobial effectiveness of chlorhexidine mouthrinse.-
dc.languageeng-
dc.publisherSage Publications, Inc.-
dc.relation.ispartofJournal of Dental Research Meeting Abstracts-
dc.rightsJournal of Dental Research Meeting Abstracts. Copyright © Sage Publications, Inc.-
dc.subjectChlorhexidine-
dc.subjectMouthrinse-
dc.subjectOTC-
dc.subjectHong Kong-
dc.subjectAntimicrobial effectiveness-
dc.titleAntimicrobial performance of five Hong Kong OTC chlorhexidine-based mouthrinse-
dc.typeConference_Paper-
dc.identifier.emailTsoi, JKH: jkhtsoi@hku.hk-
dc.identifier.emailCheong, CHL: cheongch@hkucc.hku.hk-
dc.identifier.emailWong, RWK: fyoung@hku.hk-
dc.identifier.emailMcGrath, C: mcgrathc@hkucc.hku.hk-
dc.identifier.emailKing, N: hhdbknm@hkucc.hku.hk-
dc.identifier.authorityTsoi, JKH=rp01609-
dc.identifier.authorityCheong, CHL=rp00034-
dc.identifier.authorityWong, RWK=rp00038-
dc.identifier.authorityMcGrath, C=rp00037-
dc.identifier.authorityKing, N=rp00006-
dc.identifier.hkuros242760-
dc.identifier.hkuros245686-
dc.identifier.volume94-
dc.identifier.issueSpec. Iss. A-
dc.publisher.placeUnited States-

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