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Article: Enzymatic renrichment of C20 cis-5 polyunsaturated fatty acids from biota orientalis seed oil

TitleEnzymatic renrichment of C20 cis-5 polyunsaturated fatty acids from biota orientalis seed oil
Authors
Issue Date1995
PublisherSpringer New York LLC.
Citation
Journal of Oil & Fat Industries, 1995, v. 72 n. 2, p. 245-249 How to Cite?
AbstractEnrichment ofcis-5 polyunsaturated fatty acids [20:3(5c,11c,14c), 4.3% and 20:4(5c,11c,14c,17c), 11.3%] fromBiota orientalis seed oil was carried out by lipase-catalyzed selective esterification and hydrolysis reactions. Lipases fromRhizomucor miehei (Lipozyme),Candida cylindracea and porcine pancreas were used. Lipozyme-catalyzed esterification ofBiota fatty acids withn-butanol inn-hexane allowed 20:3 and 20:4 (as fatty acids) to be enriched to a maximum level of 52.9%, and in the presence ofC. cylindracea lipase 61.5% enrichment was achieved. Esterification with pancreatic lipase was poor with low levels of enrichment of 20:3 and 20:4 (22%). A multigram scale esterification of the free fatty acids fromBiota seed oil by repeated treatment of the isolated fatty acid fraction withn-butanol inn-hexane in the presence ofC. cylindracea lipase furnished an enrichment yield of 72.5% of a mixture of 20:3 and 20:4 fatty acids. Urea fractionation of the free fatty acids ofBiota oil gave an initial enriched fraction of 20:3 (9.5%) and 20:4 (25.2%) which, upon treatment withC. cylindracea lipase inn-butanol andn-hexane, gave an enriched fraction of 85.3% of 20:3 and 20:4 fatty acids. Partial hydrolysis of the triglycerides ofBiota oil byC. cylindracea lipase in potassium phosphate buffer at 25°C resulted in a 2.8-fold enrichment ofcis-5 polyunsaturated fatty acids (40.8% of 20:3 and 20:4) as contained in the unhydrolyzed acylglycerol fractions.
Persistent Identifierhttp://hdl.handle.net/10722/209159
ISSN

 

DC FieldValueLanguage
dc.contributor.authorLie Ken Jie, MSF-
dc.contributor.authorSyed Rahmatullah, MSK-
dc.date.accessioned2015-04-01T07:00:11Z-
dc.date.available2015-04-01T07:00:11Z-
dc.date.issued1995-
dc.identifier.citationJournal of Oil & Fat Industries, 1995, v. 72 n. 2, p. 245-249-
dc.identifier.issn0095-9774-
dc.identifier.urihttp://hdl.handle.net/10722/209159-
dc.description.abstractEnrichment ofcis-5 polyunsaturated fatty acids [20:3(5c,11c,14c), 4.3% and 20:4(5c,11c,14c,17c), 11.3%] fromBiota orientalis seed oil was carried out by lipase-catalyzed selective esterification and hydrolysis reactions. Lipases fromRhizomucor miehei (Lipozyme),Candida cylindracea and porcine pancreas were used. Lipozyme-catalyzed esterification ofBiota fatty acids withn-butanol inn-hexane allowed 20:3 and 20:4 (as fatty acids) to be enriched to a maximum level of 52.9%, and in the presence ofC. cylindracea lipase 61.5% enrichment was achieved. Esterification with pancreatic lipase was poor with low levels of enrichment of 20:3 and 20:4 (22%). A multigram scale esterification of the free fatty acids fromBiota seed oil by repeated treatment of the isolated fatty acid fraction withn-butanol inn-hexane in the presence ofC. cylindracea lipase furnished an enrichment yield of 72.5% of a mixture of 20:3 and 20:4 fatty acids. Urea fractionation of the free fatty acids ofBiota oil gave an initial enriched fraction of 20:3 (9.5%) and 20:4 (25.2%) which, upon treatment withC. cylindracea lipase inn-butanol andn-hexane, gave an enriched fraction of 85.3% of 20:3 and 20:4 fatty acids. Partial hydrolysis of the triglycerides ofBiota oil byC. cylindracea lipase in potassium phosphate buffer at 25°C resulted in a 2.8-fold enrichment ofcis-5 polyunsaturated fatty acids (40.8% of 20:3 and 20:4) as contained in the unhydrolyzed acylglycerol fractions.-
dc.languageeng-
dc.publisherSpringer New York LLC.-
dc.relation.ispartofJournal of Oil & Fat Industries-
dc.rightsThe original publication is available at www.springerlink.com-
dc.titleEnzymatic renrichment of C20 cis-5 polyunsaturated fatty acids from biota orientalis seed oil-
dc.typeArticle-
dc.identifier.emailLie Ken Jie, MSF: hrsclkj@hku.hk-
dc.identifier.doi10.1007/BF02638907-
dc.identifier.hkuros514-
dc.identifier.volume72-
dc.identifier.issue2-
dc.identifier.spage245-
dc.identifier.epage249-
dc.publisher.placeUnited States-

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