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Article: Identification of a 12.5 KD protein from caudate-putamen nucleus as a dopaminergic neuronotrophic factor

TitleIdentification of a 12.5 KD protein from caudate-putamen nucleus as a dopaminergic neuronotrophic factor
Authors
Issue Date1994
PublisherScience in China Press.
Citation
Science in China Series B: Chemistry, 1994, v. 37 n. 11, p. 1360-1365 How to Cite?
AbstractThe effect of the extract from caudate-putamen nucleus of newborn rats (CPe) on the dopaminergic (DA) neurons has been studied. An MTT colorimetric microassay measuring optical densities for the growth of DA neuron cultures reveals a significant increase in growth for cultures with CPe as compared to those without CPe (p < 0.05). Rhodamine retrograde-prelabelled DA neurons were cultured on a Phastgel containing all the electrophoretically separated protein bands from CPe, and able to fish out from it their own trophic factor, a 12.5-kD protein band. The survived neurons at the 12.5-kD protein band were immunostained positive with anti-dopamine antibody. Co-culturing the 12.5 kD-containing gel strip with explants from substantia nigra at a close distance for a week revealed anti-dopamine immunopositive neurites outgrowing from the explants only towards the 12.5-kD gel strip. These results indicate that a 12.5-kD protein from the CPe is capable of maintaining the survival of DA neurons of the substantia nigra and promoting their neurite outgrowth.
Persistent Identifierhttp://hdl.handle.net/10722/209148
ISSN
2011 Impact Factor: 1.199

 

DC FieldValueLanguage
dc.contributor.authorChau, RMW-
dc.contributor.authorRen, F-
dc.contributor.authorZhao, LP-
dc.date.accessioned2015-04-01T01:22:19Z-
dc.date.available2015-04-01T01:22:19Z-
dc.date.issued1994-
dc.identifier.citationScience in China Series B: Chemistry, 1994, v. 37 n. 11, p. 1360-1365-
dc.identifier.issn1006-9291-
dc.identifier.urihttp://hdl.handle.net/10722/209148-
dc.description.abstractThe effect of the extract from caudate-putamen nucleus of newborn rats (CPe) on the dopaminergic (DA) neurons has been studied. An MTT colorimetric microassay measuring optical densities for the growth of DA neuron cultures reveals a significant increase in growth for cultures with CPe as compared to those without CPe (p < 0.05). Rhodamine retrograde-prelabelled DA neurons were cultured on a Phastgel containing all the electrophoretically separated protein bands from CPe, and able to fish out from it their own trophic factor, a 12.5-kD protein band. The survived neurons at the 12.5-kD protein band were immunostained positive with anti-dopamine antibody. Co-culturing the 12.5 kD-containing gel strip with explants from substantia nigra at a close distance for a week revealed anti-dopamine immunopositive neurites outgrowing from the explants only towards the 12.5-kD gel strip. These results indicate that a 12.5-kD protein from the CPe is capable of maintaining the survival of DA neurons of the substantia nigra and promoting their neurite outgrowth.-
dc.languageeng-
dc.publisherScience in China Press. -
dc.relation.ispartofScience in China Series B: Chemistry-
dc.rightsThe original publication is available at www.scichina.com and www.springerlink.com-
dc.titleIdentification of a 12.5 KD protein from caudate-putamen nucleus as a dopaminergic neuronotrophic factor-
dc.typeArticle-
dc.identifier.emailChau, RMW: chaurmw@hkucc.hku.hk-
dc.identifier.pmid7865127-
dc.identifier.hkuros435-
dc.identifier.volume37-
dc.identifier.issue11-
dc.identifier.spage1360-
dc.identifier.epage1365-
dc.publisher.placeBeijing, China-

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