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- Publisher Website: 10.1016/j.cellbi.2009.08.008
- Scopus: eid_2-s2.0-70350605496
- PMID: 19729070
- WOS: WOS:000271517400009
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Article: Generation and characterization of functional cardiomyocytes using induced pluripotent stem cells derived from human fibroblasts
Title | Generation and characterization of functional cardiomyocytes using induced pluripotent stem cells derived from human fibroblasts |
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Authors | |
Keywords | Cardiomyocytes Differentiation Embryonic stem cells Induced pluripotent stem cells Somatic cell reprogramming |
Issue Date | 2009 |
Publisher | Portland Press Ltd.. The Journal's web site is located at http://www.cellbiolint.org/cbi/default.htm |
Citation | Cell Biology International, 2009, v. 33 n. 11, p. 1184-1193 How to Cite? |
Abstract | We have successfully developed both spontaneous and inductive cardiomyocyte differentiation of iPS cells reprogrammed from human foreskin fibroblasts. The reprogrammed iPS cells morphologically resemble human cardiomyocytes which can beat. RT-PCR and immunostaining show that cardiac markers are expressed that are comparable to the differentiation pattern of authentic human embryonic stem cells, indicating the existence of both immature and mature differentiated cardiomyocytes. 5-Azacytidine greatly enhanced the efficiency of cardiomyocyte differentiation, whereas dimethylsulfoxide had no effect. Low serum and bone morphogenetic protein-2 marginally improved differentiation efficiency. iPS cell-derived cardiomyocytes changed their beat frequency in response to cardiac drugs, which included ion channel blockers and alpha/beta adrenergic stimulators. Derived cardiomyocytes look promising as an in vitro system for potential drug screen and/or toxicity, making this system closer to practical use in the near future. |
Persistent Identifier | http://hdl.handle.net/10722/208086 |
ISSN | 2021 Impact Factor: 4.473 2020 SCImago Journal Rankings: 0.932 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Gai, G | - |
dc.contributor.author | Leung, ELH | - |
dc.contributor.author | Costantino, PD | - |
dc.contributor.author | Aguila, JR | - |
dc.contributor.author | Nguyen, DM | - |
dc.contributor.author | Fink, LM | - |
dc.contributor.author | Ward, DC | - |
dc.contributor.author | Ma, Y | - |
dc.date.accessioned | 2015-02-10T07:21:30Z | - |
dc.date.available | 2015-02-10T07:21:30Z | - |
dc.date.issued | 2009 | - |
dc.identifier.citation | Cell Biology International, 2009, v. 33 n. 11, p. 1184-1193 | - |
dc.identifier.issn | 1065-6995 | - |
dc.identifier.uri | http://hdl.handle.net/10722/208086 | - |
dc.description.abstract | We have successfully developed both spontaneous and inductive cardiomyocyte differentiation of iPS cells reprogrammed from human foreskin fibroblasts. The reprogrammed iPS cells morphologically resemble human cardiomyocytes which can beat. RT-PCR and immunostaining show that cardiac markers are expressed that are comparable to the differentiation pattern of authentic human embryonic stem cells, indicating the existence of both immature and mature differentiated cardiomyocytes. 5-Azacytidine greatly enhanced the efficiency of cardiomyocyte differentiation, whereas dimethylsulfoxide had no effect. Low serum and bone morphogenetic protein-2 marginally improved differentiation efficiency. iPS cell-derived cardiomyocytes changed their beat frequency in response to cardiac drugs, which included ion channel blockers and alpha/beta adrenergic stimulators. Derived cardiomyocytes look promising as an in vitro system for potential drug screen and/or toxicity, making this system closer to practical use in the near future. | - |
dc.language | eng | - |
dc.publisher | Portland Press Ltd.. The Journal's web site is located at http://www.cellbiolint.org/cbi/default.htm | - |
dc.relation.ispartof | Cell Biology International | - |
dc.rights | The final version of record is available at [http://www.cellbiolint.org/cbi/default.htm]. | - |
dc.subject | Cardiomyocytes | - |
dc.subject | Differentiation | - |
dc.subject | Embryonic stem cells | - |
dc.subject | Induced pluripotent stem cells | - |
dc.subject | Somatic cell reprogramming | - |
dc.subject.mesh | Cell Differentiation - drug effects | - |
dc.subject.mesh | Fibroblasts - cytology - physiology | - |
dc.subject.mesh | Induced Pluripotent Stem Cells - cytology - physiology | - |
dc.subject.mesh | Myocytes, Cardiac - cytology - physiology | - |
dc.subject.mesh | Receptors, Adrenergic - drug effects - physiology | - |
dc.title | Generation and characterization of functional cardiomyocytes using induced pluripotent stem cells derived from human fibroblasts | en_US |
dc.type | Article | en_US |
dc.identifier.email | Leung, ELH: laihanl@yahoo.com | - |
dc.identifier.doi | 10.1016/j.cellbi.2009.08.008 | - |
dc.identifier.pmid | 19729070 | - |
dc.identifier.scopus | eid_2-s2.0-70350605496 | - |
dc.identifier.hkuros | 171439 | - |
dc.identifier.volume | 33 | - |
dc.identifier.issue | 11 | - |
dc.identifier.spage | 1184 | - |
dc.identifier.epage | 1193 | - |
dc.identifier.isi | WOS:000271517400009 | - |
dc.publisher.place | United Kingdom | - |
dc.identifier.issnl | 1065-6995 | - |