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Article: Generation and characterization of functional cardiomyocytes using induced pluripotent stem cells derived from human fibroblasts

TitleGeneration and characterization of functional cardiomyocytes using induced pluripotent stem cells derived from human fibroblasts
Authors
Issue Date2009
PublisherPortland Press Ltd.. The Journal's web site is located at http://www.cellbiolint.org/cbi/default.htm
Citation
Cell Biology International, 2009, v. 33 n. 11, p. 1184-1193 How to Cite?
AbstractWe have successfully developed both spontaneous and inductive cardiomyocyte differentiation of iPS cells reprogrammed from human foreskin fibroblasts. The reprogrammed iPS cells morphologically resemble human cardiomyocytes which can beat. RT-PCR and immunostaining show that cardiac markers are expressed that are comparable to the differentiation pattern of authentic human embryonic stem cells, indicating the existence of both immature and mature differentiated cardiomyocytes. 5-Azacytidine greatly enhanced the efficiency of cardiomyocyte differentiation, whereas dimethylsulfoxide had no effect. Low serum and bone morphogenetic protein-2 marginally improved differentiation efficiency. iPS cell-derived cardiomyocytes changed their beat frequency in response to cardiac drugs, which included ion channel blockers and alpha/beta adrenergic stimulators. Derived cardiomyocytes look promising as an in vitro system for potential drug screen and/or toxicity, making this system closer to practical use in the near future.
Persistent Identifierhttp://hdl.handle.net/10722/208086
ISSN
2015 Impact Factor: 1.663
2015 SCImago Journal Rankings: 0.705

 

DC FieldValueLanguage
dc.contributor.authorGai, G-
dc.contributor.authorLeung, ELH-
dc.contributor.authorCostantino, PD-
dc.contributor.authorAguila, JR-
dc.contributor.authorNguyen, DM-
dc.contributor.authorFink, LM-
dc.contributor.authorWard, DC-
dc.contributor.authorMa, Y-
dc.date.accessioned2015-02-10T07:21:30Z-
dc.date.available2015-02-10T07:21:30Z-
dc.date.issued2009-
dc.identifier.citationCell Biology International, 2009, v. 33 n. 11, p. 1184-1193-
dc.identifier.issn1065-6995-
dc.identifier.urihttp://hdl.handle.net/10722/208086-
dc.description.abstractWe have successfully developed both spontaneous and inductive cardiomyocyte differentiation of iPS cells reprogrammed from human foreskin fibroblasts. The reprogrammed iPS cells morphologically resemble human cardiomyocytes which can beat. RT-PCR and immunostaining show that cardiac markers are expressed that are comparable to the differentiation pattern of authentic human embryonic stem cells, indicating the existence of both immature and mature differentiated cardiomyocytes. 5-Azacytidine greatly enhanced the efficiency of cardiomyocyte differentiation, whereas dimethylsulfoxide had no effect. Low serum and bone morphogenetic protein-2 marginally improved differentiation efficiency. iPS cell-derived cardiomyocytes changed their beat frequency in response to cardiac drugs, which included ion channel blockers and alpha/beta adrenergic stimulators. Derived cardiomyocytes look promising as an in vitro system for potential drug screen and/or toxicity, making this system closer to practical use in the near future.-
dc.languageeng-
dc.publisherPortland Press Ltd.. The Journal's web site is located at http://www.cellbiolint.org/cbi/default.htm-
dc.relation.ispartofCell Biology International-
dc.rightsThe final version of record is available at [http://www.cellbiolint.org/cbi/default.htm].-
dc.subject.meshCell Differentiation - drug effects-
dc.subject.meshFibroblasts - cytology - physiology-
dc.subject.meshInduced Pluripotent Stem Cells - cytology - physiology-
dc.subject.meshMyocytes, Cardiac - cytology - physiology-
dc.subject.meshReceptors, Adrenergic - drug effects - physiology-
dc.titleGeneration and characterization of functional cardiomyocytes using induced pluripotent stem cells derived from human fibroblastsen_US
dc.typeArticleen_US
dc.identifier.emailLeung, ELH: laihanl@yahoo.com-
dc.identifier.doi10.1016/j.cellbi.2009.08.008-
dc.identifier.pmid19729070-
dc.identifier.hkuros171439-
dc.identifier.volume33-
dc.identifier.issue11-
dc.identifier.spage1184-
dc.identifier.epage1193-
dc.publisher.placeUnited Kingdom-

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