File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Characteristics of anaphylaxis-inducing IgG immune complexes triggering murine passive systemic anaphylaxis

TitleCharacteristics of anaphylaxis-inducing IgG immune complexes triggering murine passive systemic anaphylaxis
Authors
Keywordspassive systemic anaphylaxis
IgG
immune complex
monoclonal antibody
Issue Date2013
Citation
Allergy: European Journal of Allergy and Clinical Immunology, 2013, v. 68, n. 2, p. 236-245 How to Cite?
AbstractBackground With the broad and increasing application of therapeutic monoclonal antibodies (mAbs) in clinical settings, IgG-induced allergic reactions, including passive systemic anaphylaxis (PSA), have attracted significant attention. However, it is not clear which types of IgG mAb-antigen complexes or IgG aggregates formed by antigen binding can trigger PSA, as not all immune complexes (ICs) are capable of triggering PSA. Here, we characterise mAb-antigen complexes capable of inducing murine PSA to evaluate and predict which ICs are able to induce PSA. Methods Thirty-six combinatory reactions with eight antigens and 27 corresponding mAbs were used to trigger PSA, which was defined by rectal temperature. Sandwich ELISA, passive cutaneous anaphylaxis (PCA) induction and flow cytometry analysis of CD16/32 (FcγRIII/II) expression were used to characterise the ICs. The dynamic concentrations of antigen in the peripheral blood were measured by ELISA. Results Only 14 of the 36 ICs could trigger PSA and thus be termed anaphylaxis-inducing immune complexes (Ai-ICs). The Ai-ICs could be characterised by constructing sandwich ELISA, inducing PCA and down-regulating CD16/32 (FcγRIII/II) expression on blood neutrophils in vitro and in vivo. Additionally, the occurrence and severity of PSA was found to be associated with the instantaneous concentration of antigen in the peripheral blood in the presence of antibody. Conclusions Only Ai-ICs, not all ICs, could trigger IgG-mediated PSA, which could be characterised by the above simple methods. The occurrence and severity of PSA was associated with the instantaneous concentration of antigen in the peripheral blood in the presence of antibody. © 2012 John Wiley & Sons A/S.
Persistent Identifierhttp://hdl.handle.net/10722/207921
ISSN
2015 Impact Factor: 6.335
2015 SCImago Journal Rankings: 3.048

 

DC FieldValueLanguage
dc.contributor.authorJiao, DL-
dc.contributor.authorLiu, Y-
dc.contributor.authorLu, X-
dc.contributor.authorPan, QJ-
dc.contributor.authorZheng, J-
dc.contributor.authorLiu, BY-
dc.contributor.authorLiu, Y-
dc.contributor.authorWang, YD-
dc.contributor.authorFu, N-
dc.date.accessioned2015-01-26T11:46:43Z-
dc.date.available2015-01-26T11:46:43Z-
dc.date.issued2013-
dc.identifier.citationAllergy: European Journal of Allergy and Clinical Immunology, 2013, v. 68, n. 2, p. 236-245-
dc.identifier.issn0105-4538-
dc.identifier.urihttp://hdl.handle.net/10722/207921-
dc.description.abstractBackground With the broad and increasing application of therapeutic monoclonal antibodies (mAbs) in clinical settings, IgG-induced allergic reactions, including passive systemic anaphylaxis (PSA), have attracted significant attention. However, it is not clear which types of IgG mAb-antigen complexes or IgG aggregates formed by antigen binding can trigger PSA, as not all immune complexes (ICs) are capable of triggering PSA. Here, we characterise mAb-antigen complexes capable of inducing murine PSA to evaluate and predict which ICs are able to induce PSA. Methods Thirty-six combinatory reactions with eight antigens and 27 corresponding mAbs were used to trigger PSA, which was defined by rectal temperature. Sandwich ELISA, passive cutaneous anaphylaxis (PCA) induction and flow cytometry analysis of CD16/32 (FcγRIII/II) expression were used to characterise the ICs. The dynamic concentrations of antigen in the peripheral blood were measured by ELISA. Results Only 14 of the 36 ICs could trigger PSA and thus be termed anaphylaxis-inducing immune complexes (Ai-ICs). The Ai-ICs could be characterised by constructing sandwich ELISA, inducing PCA and down-regulating CD16/32 (FcγRIII/II) expression on blood neutrophils in vitro and in vivo. Additionally, the occurrence and severity of PSA was found to be associated with the instantaneous concentration of antigen in the peripheral blood in the presence of antibody. Conclusions Only Ai-ICs, not all ICs, could trigger IgG-mediated PSA, which could be characterised by the above simple methods. The occurrence and severity of PSA was associated with the instantaneous concentration of antigen in the peripheral blood in the presence of antibody. © 2012 John Wiley & Sons A/S.-
dc.languageeng-
dc.relation.ispartofAllergy: European Journal of Allergy and Clinical Immunology-
dc.subjectpassive systemic anaphylaxis-
dc.subjectIgG-
dc.subjectimmune complex-
dc.subjectmonoclonal antibody-
dc.titleCharacteristics of anaphylaxis-inducing IgG immune complexes triggering murine passive systemic anaphylaxis-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1111/all.12089-
dc.identifier.pmid23252369-
dc.identifier.scopuseid_2-s2.0-84872424213-
dc.identifier.volume68-
dc.identifier.issue2-
dc.identifier.spage236-
dc.identifier.epage245-
dc.identifier.eissn1398-9995-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats