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Article: A novel molecular test for influenza B virus detection and lineage differentiation

TitleA novel molecular test for influenza B virus detection and lineage differentiation
Authors
Issue Date2014
Citation
Journal of Medical Virology, 2014, v. 86 n. 12, p. 2171-6 How to Cite?
AbstractContemporary influenza B viruses are classified into two groups known as Yamagata and Victoria lineages. The co-circulation of two viral lineages in recent years urges for a robust and simple diagnostic test for detecting influenza B viruses and for lineage differentiation. In this study, a SYBR green-based asymmetric PCR assay has been developed for influenza B virus detection. Apart from identifying influenza B virus, the assay contains sequence-specific probes for lineage differentiation. This allows identifying influenza B virus and detecting influenza B viral lineage in a single reaction. The test has been evaluated by a panel of respiratory specimens. Of 108 influenza B virus-positive specimens, 105 (97%) were positive in this assay. None of the negative control respiratory specimens were positive in the test (N = 60). Viral lineages of all samples that are positive in the assay (N = 105) can also be classified correctly. These results suggest that this assay has a potential for routine influenza B virus surveillance.
Persistent Identifierhttp://hdl.handle.net/10722/207837

 

DC FieldValueLanguage
dc.contributor.authorWong, KSCen_US
dc.contributor.authorTsang, GCHen_US
dc.contributor.authorChan, KHen_US
dc.contributor.authorLi, OTWen_US
dc.contributor.authorPeiris, JSMen_US
dc.contributor.authorPoon, LLMen_US
dc.date.accessioned2015-01-19T11:23:03Z-
dc.date.available2015-01-19T11:23:03Z-
dc.date.issued2014en_US
dc.identifier.citationJournal of Medical Virology, 2014, v. 86 n. 12, p. 2171-6en_US
dc.identifier.urihttp://hdl.handle.net/10722/207837-
dc.description.abstractContemporary influenza B viruses are classified into two groups known as Yamagata and Victoria lineages. The co-circulation of two viral lineages in recent years urges for a robust and simple diagnostic test for detecting influenza B viruses and for lineage differentiation. In this study, a SYBR green-based asymmetric PCR assay has been developed for influenza B virus detection. Apart from identifying influenza B virus, the assay contains sequence-specific probes for lineage differentiation. This allows identifying influenza B virus and detecting influenza B viral lineage in a single reaction. The test has been evaluated by a panel of respiratory specimens. Of 108 influenza B virus-positive specimens, 105 (97%) were positive in this assay. None of the negative control respiratory specimens were positive in the test (N = 60). Viral lineages of all samples that are positive in the assay (N = 105) can also be classified correctly. These results suggest that this assay has a potential for routine influenza B virus surveillance.en_US
dc.languageengen_US
dc.relation.ispartofJournal of Medical Virologyen_US
dc.titleA novel molecular test for influenza B virus detection and lineage differentiationen_US
dc.typeArticleen_US
dc.identifier.emailWong, KSC: kschloe@hku.hken_US
dc.identifier.emailChan, KH: chankh2@hkucc.hku.hken_US
dc.identifier.emailLi, OTW: litwo@hku.hken_US
dc.identifier.emailPeiris, JSM: malik@hkucc.hku.hken_US
dc.identifier.emailPoon, LLM: llmpoon@hkucc.hku.hken_US
dc.identifier.authorityChan, KH=rp01921en_US
dc.identifier.authorityPeiris, JSM=rp00410en_US
dc.identifier.authorityPoon, LLM=rp00484en_US
dc.identifier.doi10.1002/jmv.23955en_US
dc.identifier.hkuros242084en_US
dc.identifier.volume86en_US
dc.identifier.issue12en_US
dc.identifier.spage2171en_US
dc.identifier.epage6en_US

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