File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Identification and Expression Profiling of MicroRNAs in the Brain, Liver and Gonads of Marine Medaka (Oryzias melastigma) and in Response to Hypoxia

TitleIdentification and Expression Profiling of MicroRNAs in the Brain, Liver and Gonads of Marine Medaka (Oryzias melastigma) and in Response to Hypoxia
Authors
Issue Date2014
Citation
PLoS ONE, 2014, v. 9 n. 10, p. e110698 How to Cite?
AbstractThe marine medaka (Oryzias melastigma) has been increasingly used as a fish model for detecting environmental stresses and chemical contaminants in the marine environment. Recent mammalian studies have shown that environmental stresses can alter the expression profiles of microRNAs (miRNAs), leading to transgenerational effects. Here, we use high-throughput Illumina RNA sequencing (RNA-Seq) for miRNA transcriptome analysis of brain, liver, and gonads from sexually mature male and female marine medaka. A total of 128,883,806 filtered sequence reads were generated from six small RNA libraries, identifying a total of 2,125,663 non-redundant sequences. These sequences were aligned and annotated to known animal miRNAs (miRBase) using the BLAST method. A total of 223 distinct miRNA types were identified, with the greatest number expressed in brain tissue. Our data suggested that 55 miRNA types from 34 families are common to all tested tissues, while some of the miRNAs are tissue-enriched or sex-enriched. Quantitative real-time PCR analysis further demonstrated that let-7a, miR-122, and miR-9-3p were downregulated in hypoxic female medaka, while miR-2184 was specifically upregulated in the testis of hypoxic male fish. This is the first study to identify miRNAs in O. melastigma using small RNA deep sequencing technology. Because miRNA expression is highly conserved between marine medaka and other vertebrates, marine medaka may serve as a good model for studies on the functional roles of miRNAs in hypoxia stress response and signaling in marine fish.
Persistent Identifierhttp://hdl.handle.net/10722/206862
ISSN
2015 Impact Factor: 3.057
2015 SCImago Journal Rankings: 1.395
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLau, KYTen_US
dc.contributor.authorLai, KPen_US
dc.contributor.authorBao, YJen_US
dc.contributor.authorZhang, Nen_US
dc.contributor.authorTse, CKAen_US
dc.contributor.authorTong, Aen_US
dc.contributor.authorLi, JWen_US
dc.contributor.authorLok, Sen_US
dc.contributor.authorKong, YCen_US
dc.contributor.authorLui, WYen_US
dc.contributor.authorWong, A-
dc.contributor.authorWu, RSS-
dc.date.accessioned2014-12-02T10:45:12Z-
dc.date.available2014-12-02T10:45:12Z-
dc.date.issued2014en_US
dc.identifier.citationPLoS ONE, 2014, v. 9 n. 10, p. e110698en_US
dc.identifier.issn1932-6203-
dc.identifier.urihttp://hdl.handle.net/10722/206862-
dc.description.abstractThe marine medaka (Oryzias melastigma) has been increasingly used as a fish model for detecting environmental stresses and chemical contaminants in the marine environment. Recent mammalian studies have shown that environmental stresses can alter the expression profiles of microRNAs (miRNAs), leading to transgenerational effects. Here, we use high-throughput Illumina RNA sequencing (RNA-Seq) for miRNA transcriptome analysis of brain, liver, and gonads from sexually mature male and female marine medaka. A total of 128,883,806 filtered sequence reads were generated from six small RNA libraries, identifying a total of 2,125,663 non-redundant sequences. These sequences were aligned and annotated to known animal miRNAs (miRBase) using the BLAST method. A total of 223 distinct miRNA types were identified, with the greatest number expressed in brain tissue. Our data suggested that 55 miRNA types from 34 families are common to all tested tissues, while some of the miRNAs are tissue-enriched or sex-enriched. Quantitative real-time PCR analysis further demonstrated that let-7a, miR-122, and miR-9-3p were downregulated in hypoxic female medaka, while miR-2184 was specifically upregulated in the testis of hypoxic male fish. This is the first study to identify miRNAs in O. melastigma using small RNA deep sequencing technology. Because miRNA expression is highly conserved between marine medaka and other vertebrates, marine medaka may serve as a good model for studies on the functional roles of miRNAs in hypoxia stress response and signaling in marine fish.-
dc.languageengen_US
dc.relation.ispartofPLoS ONEen_US
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.titleIdentification and Expression Profiling of MicroRNAs in the Brain, Liver and Gonads of Marine Medaka (Oryzias melastigma) and in Response to Hypoxiaen_US
dc.typeArticleen_US
dc.identifier.emailLau, KYT: kytlau@hku.hken_US
dc.identifier.emailLai, KP: balllai@hku.hken_US
dc.identifier.emailTse, CKA: annatse@hku.hken_US
dc.identifier.emailLui, WY: wylui@hku.hken_US
dc.identifier.authorityLai, KP=rp01753en_US
dc.identifier.authorityLui, WY=rp00756en_US
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1371/journal.pone.0110698en_US
dc.identifier.pmid25350659-
dc.identifier.hkuros241588en_US
dc.identifier.hkuros260443-
dc.identifier.volume9en_US
dc.identifier.spagee110698en_US
dc.identifier.epagee110698en_US
dc.identifier.isiWOS:000343943100036-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats