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postgraduate thesis: Detection of human parechovirus and Saffold virus from hospitalized patients with respiratory tract infection in Hong Kong

TitleDetection of human parechovirus and Saffold virus from hospitalized patients with respiratory tract infection in Hong Kong
Authors
Issue Date2014
PublisherThe University of Hong Kong (Pokfulam, Hong Kong)
Citation
Lam, S. [藍新兒]. (2014). Detection of human parechovirus and Saffold virus from hospitalized patients with respiratory tract infection in Hong Kong. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5303946
AbstractBackground: Respiratory tract infection is one of the major diseases to cause morbidity and mortality worldwide. Undiagnosed respiratory infection remains unclear. Picornavirus is most common to cause respiratory infection after the influenza virus and RSV. There were numerous notorious pathogens in the Picornaviridae family, for instance, human parechovirus and Saffold virus. These emerging and novel viruses are reported sporadically in respiratory infection and amongst children in particular. This study is aimed to assess the potential role of HPeV and SAFV in respiratory infection in Hong Kong. Methods: Between May 2013 and April 2014, nasopharyngeal aspirates (NPA) were collected from hospitalized patients who have respiratory infection. The collected samples were tested negative for respiratory syncytial virus, adenovirus, influenza A and B viruses, parainfluenza viruses types 1, 2 and 3 by direct immunofluorescence. RT-PCR was used to target the HPeV and SAFV corresponding region of 5'UTR and analyze by the BioEdit sequence Alignment Editor and Basic Local Alignment Search Tool. Results: 597 female and 603 male were included in 1200 NPA samples. 20% of these samples were under the age of 5. However, there were no HPeV and SAFV detected in all 1200 NPA samples. Conclusion: To reveal the possible association between viruses and respiratory infection, the sampling size and district area should be expanded. The single detection method may not be able to detect all the viruses in the current study.
DegreeMaster of Medical Sciences
SubjectRespiratory infections - Diagnosis - China - Hong Kong
Picornaviruses - China - Hong Kong
Dept/ProgramMicrobiology
Persistent Identifierhttp://hdl.handle.net/10722/206510

 

DC FieldValueLanguage
dc.contributor.authorLam, Sun-yee-
dc.contributor.author藍新兒-
dc.date.accessioned2014-11-03T23:14:51Z-
dc.date.available2014-11-03T23:14:51Z-
dc.date.issued2014-
dc.identifier.citationLam, S. [藍新兒]. (2014). Detection of human parechovirus and Saffold virus from hospitalized patients with respiratory tract infection in Hong Kong. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5303946-
dc.identifier.urihttp://hdl.handle.net/10722/206510-
dc.description.abstractBackground: Respiratory tract infection is one of the major diseases to cause morbidity and mortality worldwide. Undiagnosed respiratory infection remains unclear. Picornavirus is most common to cause respiratory infection after the influenza virus and RSV. There were numerous notorious pathogens in the Picornaviridae family, for instance, human parechovirus and Saffold virus. These emerging and novel viruses are reported sporadically in respiratory infection and amongst children in particular. This study is aimed to assess the potential role of HPeV and SAFV in respiratory infection in Hong Kong. Methods: Between May 2013 and April 2014, nasopharyngeal aspirates (NPA) were collected from hospitalized patients who have respiratory infection. The collected samples were tested negative for respiratory syncytial virus, adenovirus, influenza A and B viruses, parainfluenza viruses types 1, 2 and 3 by direct immunofluorescence. RT-PCR was used to target the HPeV and SAFV corresponding region of 5'UTR and analyze by the BioEdit sequence Alignment Editor and Basic Local Alignment Search Tool. Results: 597 female and 603 male were included in 1200 NPA samples. 20% of these samples were under the age of 5. However, there were no HPeV and SAFV detected in all 1200 NPA samples. Conclusion: To reveal the possible association between viruses and respiratory infection, the sampling size and district area should be expanded. The single detection method may not be able to detect all the viruses in the current study.-
dc.languageeng-
dc.publisherThe University of Hong Kong (Pokfulam, Hong Kong)-
dc.relation.ispartofHKU Theses Online (HKUTO)-
dc.rightsThe author retains all proprietary rights, (such as patent rights) and the right to use in future works.-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.lcshRespiratory infections - Diagnosis - China - Hong Kong-
dc.subject.lcshPicornaviruses - China - Hong Kong-
dc.titleDetection of human parechovirus and Saffold virus from hospitalized patients with respiratory tract infection in Hong Kong-
dc.typePG_Thesis-
dc.identifier.hkulb5303946-
dc.description.thesisnameMaster of Medical Sciences-
dc.description.thesislevelMaster-
dc.description.thesisdisciplineMicrobiology-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.5353/th_b5303946-

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