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Article: A study of fast and metastable dissociations of adenine-thymine binary-base oligonucleotides by using positive-ion MALDI-TOF mass spectrometry

TitleA study of fast and metastable dissociations of adenine-thymine binary-base oligonucleotides by using positive-ion MALDI-TOF mass spectrometry
Authors
Issue Date2002
Citation
Journal of the American Society for Mass Spectrometry, 2002, v. 13, n. 9, p. 1052-1064 How to Cite?
AbstractIn the present study, fast and metastable dissociations of a number of adenine-thymine binary-base oligonucleotides under the conditions of UV matrix-assisted laser desorption/ionization mass spectrometry were investigated. 2-Aminobenzoic acid/ammonium fluoride (ABA/NH4F) matrix system was used. The spectra obtained under metastable and fast dissociation conditions exhibit distinctive dissociation products. From the post-source-decay analysis, all oligonucleotides underwent predominantly metastable dissociations at the 3′ C-O linkages to form [an-B]+ and wn + complimentary ion series. Based on the present results, the so-called "[wn+80]+" ions were postulated to be the complimentary [z(8-n)AH]+ ions rather than the expected phosphate rearrangement products. In addition, these oligonucleotides were found to generate fast dissociation products of bn +, dn +, wn +and yn + ions through backbone cleavages at 5′ C-O, 5′ O-P, 3′ C-O and 3′ P-O linkages, respectively. Product ion series formed under PSD conditions were not observed. The implications of this mutually exclusive occurrence of the two sets of fragment ions under fast and metastable conditions using ABA/NH4F matrix would be discussed. A model of ion activation under UV-MALDI conditions was also proposed. © 2002 American Society for Mass Spectrometry.
Persistent Identifierhttp://hdl.handle.net/10722/206292
ISSN
2015 Impact Factor: 3.031
2015 SCImago Journal Rankings: 1.322
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChan, Tak Wah Dominic-
dc.contributor.authorFung, Yi Man Eva-
dc.contributor.authorLi, Y. C Leo-
dc.date.accessioned2014-10-22T01:25:34Z-
dc.date.available2014-10-22T01:25:34Z-
dc.date.issued2002-
dc.identifier.citationJournal of the American Society for Mass Spectrometry, 2002, v. 13, n. 9, p. 1052-1064-
dc.identifier.issn1044-0305-
dc.identifier.urihttp://hdl.handle.net/10722/206292-
dc.description.abstractIn the present study, fast and metastable dissociations of a number of adenine-thymine binary-base oligonucleotides under the conditions of UV matrix-assisted laser desorption/ionization mass spectrometry were investigated. 2-Aminobenzoic acid/ammonium fluoride (ABA/NH4F) matrix system was used. The spectra obtained under metastable and fast dissociation conditions exhibit distinctive dissociation products. From the post-source-decay analysis, all oligonucleotides underwent predominantly metastable dissociations at the 3′ C-O linkages to form [an-B]+ and wn + complimentary ion series. Based on the present results, the so-called "[wn+80]+" ions were postulated to be the complimentary [z(8-n)AH]+ ions rather than the expected phosphate rearrangement products. In addition, these oligonucleotides were found to generate fast dissociation products of bn +, dn +, wn +and yn + ions through backbone cleavages at 5′ C-O, 5′ O-P, 3′ C-O and 3′ P-O linkages, respectively. Product ion series formed under PSD conditions were not observed. The implications of this mutually exclusive occurrence of the two sets of fragment ions under fast and metastable conditions using ABA/NH4F matrix would be discussed. A model of ion activation under UV-MALDI conditions was also proposed. © 2002 American Society for Mass Spectrometry.-
dc.languageeng-
dc.relation.ispartofJournal of the American Society for Mass Spectrometry-
dc.titleA study of fast and metastable dissociations of adenine-thymine binary-base oligonucleotides by using positive-ion MALDI-TOF mass spectrometry-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S1044-0305(02)00423-3-
dc.identifier.pmid12322953-
dc.identifier.scopuseid_2-s2.0-0036731452-
dc.identifier.volume13-
dc.identifier.issue9-
dc.identifier.spage1052-
dc.identifier.epage1064-
dc.identifier.isiWOS:000178083200005-

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