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Article: Antioxidant and antibacterial activities of exopolysaccharides from Bifidobacterium bifidum WBIN03 and Lactobacillus plantarum R315

TitleAntioxidant and antibacterial activities of exopolysaccharides from Bifidobacterium bifidum WBIN03 and Lactobacillus plantarum R315
Authors
KeywordsAntioxidative and antibacterial activity
Bifidobacterium bifidum
Exopolysaccharide
Lactobacillus plantarum
Issue Date2014
PublisherAmerican Dairy Science Association. The Journal's web site is located at http://www.journalofdairyscience.org/
Citation
Journal of Dairy Science, 2014, v. 97 n. 12, p. 7334-7343 How to Cite?
AbstractThe objective of this study was to investigate the antioxidant and antibacterial activities of exopolysaccharide (EPS) from Bifidobacterium bifidum WBIN03 (B-EPS) and Lactobacillus plantarum R315 (L-EPS). The 1,1-diphenyl-2-picrylhydrazyl (DPPH)-radical scavenging, hydroxyl radical-scavenging, and superoxide radical-scavenging abilities were measured to evaluate antioxidant activity. Inhibition of erythrocyte hemolysis and lipid peroxidation was also measured. Both B-EPS and L-EPS had strong scavenging ability against DPPH and superoxide radicals at high concentration. The inhibitory effect of B-EPS on erythrocyte hemolysis was stronger than that of L-EPS in a concentration range from 0.30 to 1.00 mg/mL, whereas the hydroxyl scavenging ability of L-EPS (39.15 ± 0.58%) was significantly higher than that of 0.15 mg/mL ascorbic acid (24.33 ± 1.17%) and B-EPS (17.89 ± 3.30%) at 0.10 mg/mL. The inhibition of lipid peroxidation of 0.50 mg/mL B-EPS and L-EPS was 13.48 ± 1.74% and 12.43 ± 0.51%, respectively, values lower than that of ascorbic acid at the same concentration (23.20 ± 1.41%). Furthermore, all these abilities were enhanced in a concentration-dependent manner. Agar diffusion assay showed that both EPS exhibited antibacterial activities against tested pathogens such as Cronobacter sakazakii, Escherichia coli, Listeria monocytogenes, Staphyloccocus aureus, Candida albicans, Bacillus cereus, Salmonella typhimurium, and Shigella sonnei at 300 μg/mL. In conclusion, both EPS have antimicrobial and antioxidant activities and could have applications in the food industry.
Persistent Identifierhttp://hdl.handle.net/10722/206091
ISSN
2017 Impact Factor: 2.749
2015 SCImago Journal Rankings: 1.401
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLi, S-
dc.contributor.authorHuang, R-
dc.contributor.authorShah, NP-
dc.contributor.authorTao, X-
dc.contributor.authorXiong, Y-
dc.contributor.authorWei, H-
dc.date.accessioned2014-10-20T12:13:30Z-
dc.date.available2014-10-20T12:13:30Z-
dc.date.issued2014-
dc.identifier.citationJournal of Dairy Science, 2014, v. 97 n. 12, p. 7334-7343-
dc.identifier.issn0022-0302-
dc.identifier.urihttp://hdl.handle.net/10722/206091-
dc.description.abstractThe objective of this study was to investigate the antioxidant and antibacterial activities of exopolysaccharide (EPS) from Bifidobacterium bifidum WBIN03 (B-EPS) and Lactobacillus plantarum R315 (L-EPS). The 1,1-diphenyl-2-picrylhydrazyl (DPPH)-radical scavenging, hydroxyl radical-scavenging, and superoxide radical-scavenging abilities were measured to evaluate antioxidant activity. Inhibition of erythrocyte hemolysis and lipid peroxidation was also measured. Both B-EPS and L-EPS had strong scavenging ability against DPPH and superoxide radicals at high concentration. The inhibitory effect of B-EPS on erythrocyte hemolysis was stronger than that of L-EPS in a concentration range from 0.30 to 1.00 mg/mL, whereas the hydroxyl scavenging ability of L-EPS (39.15 ± 0.58%) was significantly higher than that of 0.15 mg/mL ascorbic acid (24.33 ± 1.17%) and B-EPS (17.89 ± 3.30%) at 0.10 mg/mL. The inhibition of lipid peroxidation of 0.50 mg/mL B-EPS and L-EPS was 13.48 ± 1.74% and 12.43 ± 0.51%, respectively, values lower than that of ascorbic acid at the same concentration (23.20 ± 1.41%). Furthermore, all these abilities were enhanced in a concentration-dependent manner. Agar diffusion assay showed that both EPS exhibited antibacterial activities against tested pathogens such as Cronobacter sakazakii, Escherichia coli, Listeria monocytogenes, Staphyloccocus aureus, Candida albicans, Bacillus cereus, Salmonella typhimurium, and Shigella sonnei at 300 μg/mL. In conclusion, both EPS have antimicrobial and antioxidant activities and could have applications in the food industry.-
dc.languageeng-
dc.publisherAmerican Dairy Science Association. The Journal's web site is located at http://www.journalofdairyscience.org/-
dc.relation.ispartofJournal of Dairy Science-
dc.subjectAntioxidative and antibacterial activity-
dc.subjectBifidobacterium bifidum-
dc.subjectExopolysaccharide-
dc.subjectLactobacillus plantarum-
dc.titleAntioxidant and antibacterial activities of exopolysaccharides from Bifidobacterium bifidum WBIN03 and Lactobacillus plantarum R315-
dc.typeArticle-
dc.identifier.emailShah, NP: npshah@hku.hk-
dc.identifier.authorityShah, NP=rp01571-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.3168/jds.2014-7912-
dc.identifier.pmid25282420-
dc.identifier.scopuseid_2-s2.0-84918767373-
dc.identifier.hkuros239866-
dc.identifier.volume97-
dc.identifier.issue12-
dc.identifier.spage7334-
dc.identifier.epage7343-
dc.identifier.isiWOS:000345256200004-
dc.publisher.placeUnited States-

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