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Conference Paper: The role of c-Myc in phagocytosis of mycobacteria in human macrophages

TitleThe role of c-Myc in phagocytosis of mycobacteria in human macrophages
Authors
Issue Date2013
PublisherAmerican Association of Immunologists. The Journal's web site is located at http://www.jimmunol.org
Citation
The Annual Meeting of the American Association of Immunologists (AAI) on Immunology (Immunology 2013), Honolulu, Hawaii, 3-7 May 2013. In Journal of Immunology, 2013, v. 190 n. Meeting Abstract Supplement, p. abstract no. 130.6 How to Cite?
AbstractTuberculosis remains a major cause of morbidity and mortality worldwide as a result of Mycobacterium tuberculosis infection. Macrophages are the major immunocytes to initiate host immunity against mycobacteria. Among the multiple strategies employed by macrophages to inhibit mycobacteria growth or kill intracellular mycobacteria, phagocytosis is the first step. Phagocytosis involves recognition, internalization and finally digestion of pathogens at phagolysosome. c-Myc is a transcription factor that regulates a variety of target genes. It can form a complex with Max and bind to the enhancer box sequences of the promoter to mediate the transcription. Recently, our group revealed that c-Myc has a potential role in regulating the antimicrobial responses in macrophages. Here, we further revealed that c-Myc may play a positive role in phagocytosis and contribute to host defense to mycobacteria. We demonstrated that mycobacteria could induce c-Myc expression in primary human macrophages. Using colony forming unit assay and cell imaging assay, we showed that pretreatment of c-Myc inhibitor, 10058-F4, could significantly reduce the amount of mycobacteria internalized by macrophages. In addition, the acidification of phagolysosome in mycobacteria infected macrophages was shown to be inhibited by 10058-F4. However, the action of c-Myc in the process of phagocytosis is independent of Rho family GTPases. In conclusion, c-Myc may play a role in phagocytosis of mycobacteria.
Persistent Identifierhttp://hdl.handle.net/10722/206056
ISSN
2015 Impact Factor: 4.985
2015 SCImago Journal Rankings: 3.549

 

DC FieldValueLanguage
dc.contributor.authorWang, Len_US
dc.contributor.authorLi, CBen_US
dc.contributor.authorLau, ASYen_US
dc.date.accessioned2014-10-20T11:47:25Z-
dc.date.available2014-10-20T11:47:25Z-
dc.date.issued2013en_US
dc.identifier.citationThe Annual Meeting of the American Association of Immunologists (AAI) on Immunology (Immunology 2013), Honolulu, Hawaii, 3-7 May 2013. In Journal of Immunology, 2013, v. 190 n. Meeting Abstract Supplement, p. abstract no. 130.6en_US
dc.identifier.issn0022-1767-
dc.identifier.urihttp://hdl.handle.net/10722/206056-
dc.description.abstractTuberculosis remains a major cause of morbidity and mortality worldwide as a result of Mycobacterium tuberculosis infection. Macrophages are the major immunocytes to initiate host immunity against mycobacteria. Among the multiple strategies employed by macrophages to inhibit mycobacteria growth or kill intracellular mycobacteria, phagocytosis is the first step. Phagocytosis involves recognition, internalization and finally digestion of pathogens at phagolysosome. c-Myc is a transcription factor that regulates a variety of target genes. It can form a complex with Max and bind to the enhancer box sequences of the promoter to mediate the transcription. Recently, our group revealed that c-Myc has a potential role in regulating the antimicrobial responses in macrophages. Here, we further revealed that c-Myc may play a positive role in phagocytosis and contribute to host defense to mycobacteria. We demonstrated that mycobacteria could induce c-Myc expression in primary human macrophages. Using colony forming unit assay and cell imaging assay, we showed that pretreatment of c-Myc inhibitor, 10058-F4, could significantly reduce the amount of mycobacteria internalized by macrophages. In addition, the acidification of phagolysosome in mycobacteria infected macrophages was shown to be inhibited by 10058-F4. However, the action of c-Myc in the process of phagocytosis is independent of Rho family GTPases. In conclusion, c-Myc may play a role in phagocytosis of mycobacteria.-
dc.languageengen_US
dc.publisherAmerican Association of Immunologists. The Journal's web site is located at http://www.jimmunol.org-
dc.relation.ispartofJournal of Immunologyen_US
dc.titleThe role of c-Myc in phagocytosis of mycobacteria in human macrophagesen_US
dc.typeConference_Paperen_US
dc.identifier.emailWang, L: vickyw@hku.hken_US
dc.identifier.emailLi, CB: jamesli@graduate.hku.hken_US
dc.identifier.emailLau, ASY: asylau@hku.hken_US
dc.identifier.authorityLi, CB=rp00496en_US
dc.identifier.authorityLau, ASY=rp00474en_US
dc.identifier.hkuros241336en_US
dc.identifier.volume190-
dc.identifier.issueMeeting Abstract Supplement-
dc.publisher.placeUnited States-

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