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Article: Assessment of the rate of spinal motor axon regeneration by choline acetyltransferase immunohistochemistry following sciatic nerve crush injury in mice: Laboratory investigation

TitleAssessment of the rate of spinal motor axon regeneration by choline acetyltransferase immunohistochemistry following sciatic nerve crush injury in mice: Laboratory investigation
Authors
KeywordsNerve injury
Motor neuron
Axon regeneration
ChAT staining
Peripheral nerve
Issue Date2014
Citation
Journal of Neurosurgery, 2014, v. 120, n. 2, p. 502-508 How to Cite?
AbstractObject. The purpose of this study was to examine whether choline acetyl transferase (ChAT) staining can be used for assessing the rate of motor neuron regeneration at an early phase of axon outgrowth. Methods. The authors developed a new sciatic nerve crush model in adult mice. In this model, in addition to performing a sciatic nerve crush injury, the authors excised the ipsilateral lumbar L3-6 dorsal root ganglion (DRG), which resulted in degeneration of the sensory fibers entering into the sciatic nerve. Crushed nerve sections obtained at Day 3 or Day 7 postinjury were analyzed by means of immunostaining. Results. The immunostaining showed that ChAT, a motor axon-specific antigen, was totally co-localized with growth-associated protein 43 (GAP-43), which is expressed in regenerating nerves and transported into growth cones. Conclusions. Our results suggest that measuring the length of motor axon outgrowth by ChAT immunostaining is reliable. ChAT staining provides a more convenient method for evaluating the rate of motor axon outgrowth in a mixed nerve. ©AANS, 2014.
Persistent Identifierhttp://hdl.handle.net/10722/205802
ISSN
2015 Impact Factor: 3.443
2015 SCImago Journal Rankings: 1.673
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorYuan, Qiuju-
dc.contributor.authorSu, Huanxing-
dc.contributor.authorChiu, Kin-
dc.contributor.authorLin, Zhixiu-
dc.contributor.authorWu, Wutian-
dc.date.accessioned2014-10-06T08:02:23Z-
dc.date.available2014-10-06T08:02:23Z-
dc.date.issued2014-
dc.identifier.citationJournal of Neurosurgery, 2014, v. 120, n. 2, p. 502-508-
dc.identifier.issn0022-3085-
dc.identifier.urihttp://hdl.handle.net/10722/205802-
dc.description.abstractObject. The purpose of this study was to examine whether choline acetyl transferase (ChAT) staining can be used for assessing the rate of motor neuron regeneration at an early phase of axon outgrowth. Methods. The authors developed a new sciatic nerve crush model in adult mice. In this model, in addition to performing a sciatic nerve crush injury, the authors excised the ipsilateral lumbar L3-6 dorsal root ganglion (DRG), which resulted in degeneration of the sensory fibers entering into the sciatic nerve. Crushed nerve sections obtained at Day 3 or Day 7 postinjury were analyzed by means of immunostaining. Results. The immunostaining showed that ChAT, a motor axon-specific antigen, was totally co-localized with growth-associated protein 43 (GAP-43), which is expressed in regenerating nerves and transported into growth cones. Conclusions. Our results suggest that measuring the length of motor axon outgrowth by ChAT immunostaining is reliable. ChAT staining provides a more convenient method for evaluating the rate of motor axon outgrowth in a mixed nerve. ©AANS, 2014.-
dc.languageeng-
dc.relation.ispartofJournal of Neurosurgery-
dc.subjectNerve injury-
dc.subjectMotor neuron-
dc.subjectAxon regeneration-
dc.subjectChAT staining-
dc.subjectPeripheral nerve-
dc.titleAssessment of the rate of spinal motor axon regeneration by choline acetyltransferase immunohistochemistry following sciatic nerve crush injury in mice: Laboratory investigation-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.3171/2013.8.JNS121648-
dc.identifier.pmid24032704-
dc.identifier.scopuseid_2-s2.0-84893269071-
dc.identifier.hkuros236435-
dc.identifier.volume120-
dc.identifier.issue2-
dc.identifier.spage502-
dc.identifier.epage508-
dc.identifier.eissn1933-0693-
dc.identifier.isiWOS:000330556400034-

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