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Article: Delayed administration of bone marrow mesenchymal stem cell conditioned medium significantly improves outcome after retinal ischemia in rats

TitleDelayed administration of bone marrow mesenchymal stem cell conditioned medium significantly improves outcome after retinal ischemia in rats
Authors
Issue Date2014
Citation
Investigative Ophthalmology & Visual Science, 2014, v. 55 n. 6, p. 3785-96 How to Cite?
AbstractPURPOSE: Delayed treatment after ischemia is often unsatisfactory. We hypothesized that injection of bone marrow stem cell (BMSC) conditioned medium after ischemia could rescue ischemic retina, and in this study we characterized the functional and histological outcomes and mechanisms of this neuroprotection. METHODS: Retinal ischemia was produced in adult Wistar rats by increasing intraocular pressure for 55 minutes. Conditioned medium (CM) from rat BMSCs or unconditioned medium (uCM) was injected into the vitreous 24 hours after the end of ischemia. Recovery was assessed 7 days after ischemia using electroretinography, at which time we euthanized the animals and then prepared 4-mum-thick paraffin-embedded retinal sections. TUNEL and Western blot were used to identify apoptotic cells and apoptosis-related gene expression 24 hours after injections; that is, 48 hours after ischemia. Protein content in CM versus uCM was studied using tandem mass spectrometry, and bioinformatics methods were used to model protein interactions. RESULTS: Intravitreal injection of CM 24 hours after ischemia significantly improved retinal function and attenuated cell loss in the retinal ganglion cell layer. CM attenuated postischemic apoptosis and apoptosis-related gene expression. By spectral counting, 19 proteins that met stringent identification criteria were increased in the CM compared to uCM; the majority were extracellular matrix proteins that mapped into an interactional network together with other proteins involved in cell growth and adhesion. CONCLUSIONS: By restoring retinal function, attenuating apoptosis, and preventing retinal cell loss after ischemia, CM is a robust means of delayed postischemic intervention. We identified some potential candidate proteins for this effect.
Persistent Identifierhttp://hdl.handle.net/10722/205564
ISSN
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorDreixler, JCen_US
dc.contributor.authorPoston, JNen_US
dc.contributor.authorBalyasnikova, Ien_US
dc.contributor.authorShaikh, ARen_US
dc.contributor.authorTupper, KYen_US
dc.contributor.authorConway, Sen_US
dc.contributor.authorBoddapati, Ven_US
dc.contributor.authorMarcet, MMen_US
dc.contributor.authorLesniak, MSen_US
dc.contributor.authorRoth, Sen_US
dc.date.accessioned2014-09-20T04:00:21Z-
dc.date.available2014-09-20T04:00:21Z-
dc.date.issued2014en_US
dc.identifier.citationInvestigative Ophthalmology & Visual Science, 2014, v. 55 n. 6, p. 3785-96en_US
dc.identifier.issn9780240811376en_US
dc.identifier.urihttp://hdl.handle.net/10722/205564-
dc.description.abstractPURPOSE: Delayed treatment after ischemia is often unsatisfactory. We hypothesized that injection of bone marrow stem cell (BMSC) conditioned medium after ischemia could rescue ischemic retina, and in this study we characterized the functional and histological outcomes and mechanisms of this neuroprotection. METHODS: Retinal ischemia was produced in adult Wistar rats by increasing intraocular pressure for 55 minutes. Conditioned medium (CM) from rat BMSCs or unconditioned medium (uCM) was injected into the vitreous 24 hours after the end of ischemia. Recovery was assessed 7 days after ischemia using electroretinography, at which time we euthanized the animals and then prepared 4-mum-thick paraffin-embedded retinal sections. TUNEL and Western blot were used to identify apoptotic cells and apoptosis-related gene expression 24 hours after injections; that is, 48 hours after ischemia. Protein content in CM versus uCM was studied using tandem mass spectrometry, and bioinformatics methods were used to model protein interactions. RESULTS: Intravitreal injection of CM 24 hours after ischemia significantly improved retinal function and attenuated cell loss in the retinal ganglion cell layer. CM attenuated postischemic apoptosis and apoptosis-related gene expression. By spectral counting, 19 proteins that met stringent identification criteria were increased in the CM compared to uCM; the majority were extracellular matrix proteins that mapped into an interactional network together with other proteins involved in cell growth and adhesion. CONCLUSIONS: By restoring retinal function, attenuating apoptosis, and preventing retinal cell loss after ischemia, CM is a robust means of delayed postischemic intervention. We identified some potential candidate proteins for this effect.en_US
dc.languageengen_US
dc.relation.ispartofInvestigative Ophthalmology & Visual Scienceen_US
dc.titleDelayed administration of bone marrow mesenchymal stem cell conditioned medium significantly improves outcome after retinal ischemia in ratsen_US
dc.typeArticleen_US
dc.identifier.emailMarcet, MM: marcet@hku.hken_US
dc.identifier.authorityMarcet, MM=rp01363en_US
dc.identifier.doi10.1167/iovs.13-11683en_US
dc.identifier.pmid24699381-
dc.identifier.hkuros237836en_US
dc.identifier.volume55en_US
dc.identifier.issue6en_US
dc.identifier.spage3785en_US
dc.identifier.epage96en_US
dc.identifier.isiWOS:000339485800051-

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