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Article: Induction of serpinb1a by PACAP or NGF is required for PC12 cells survival after serum withdrawal

TitleInduction of serpinb1a by PACAP or NGF is required for PC12 cells survival after serum withdrawal
Authors
Issue Date2014
PublisherWiley-Blackwell Publishing Ltd. The Journal's web site is located at http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1471-4159
Citation
Journal of Neurochemistry, 2014, v. 131 n. 1, p. 21-32 How to Cite?
AbstractAbstract Thumbnail image of graphical abstract PC12 cells are used to study the signaling mechanisms underlying the neurotrophic and neuroprotective activities of pituitary adenylate cyclase-activating polypeptide (PACAP) and nerve growth factor (NGF). Previous microarray experiments indicated that serpinb1a was the most induced gene after 6 h of treatment with PACAP or NGF. This study confirmed that serpinb1a is strongly activated by PACAP and NGF in a time-dependent manner with a maximum induction (~ 50-fold over control) observed after 6 h of treatment. Co-incubation with PACAP and NGF resulted in a synergistic up-regulation of serpinb1a expression (200-fold over control), suggesting that PACAP and NGF act through complementary mechanisms. Consistently, PACAP-induced serpinb1a expression was not blocked by TrkA receptor inhibition. Nevertheless, the stimulation of serpinb1a expression by PACAP and NGF was significantly reduced in the presence of extracellular signal-regulated kinase, calcineurin, protein kinase A, p38, and PI3K inhibitors, indicating that the two trophic factors share some common pathways in the regulation of serpinb1a. Finally, functional investigations conducted with siRNA revealed that serpinb1a is not involved in the effects of PACAP and NGF on PC12 cell neuritogenesis, proliferation or body cell volume but mediates their ability to block caspases 3/7 activity and to promote PC12 cell survival. Pituitary adenylate cyclase-activating polypeptide (PACAP) and nerve growth factor (NGF) induce a strong increase in serpinb1 a expression in PC12 cells. Functional investigations revealed that this increase in serpinb1a does not affect cell proliferation or differentiation but inhibits caspase 3 activity and promotes cell survival.
Persistent Identifierhttp://hdl.handle.net/10722/204840
ISSN
2015 Impact Factor: 3.842
2015 SCImago Journal Rankings: 2.021
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorSeaborn, T-
dc.contributor.authorRavni, A-
dc.contributor.authorAu, R-
dc.contributor.authorChow, BKC-
dc.contributor.authorFournier, A-
dc.contributor.authorWurtz, O-
dc.contributor.authorVaudry, H-
dc.contributor.authorEiden, LE-
dc.contributor.authorVaudry, D-
dc.date.accessioned2014-09-20T00:43:28Z-
dc.date.available2014-09-20T00:43:28Z-
dc.date.issued2014-
dc.identifier.citationJournal of Neurochemistry, 2014, v. 131 n. 1, p. 21-32-
dc.identifier.issn0022-3042-
dc.identifier.urihttp://hdl.handle.net/10722/204840-
dc.description.abstractAbstract Thumbnail image of graphical abstract PC12 cells are used to study the signaling mechanisms underlying the neurotrophic and neuroprotective activities of pituitary adenylate cyclase-activating polypeptide (PACAP) and nerve growth factor (NGF). Previous microarray experiments indicated that serpinb1a was the most induced gene after 6 h of treatment with PACAP or NGF. This study confirmed that serpinb1a is strongly activated by PACAP and NGF in a time-dependent manner with a maximum induction (~ 50-fold over control) observed after 6 h of treatment. Co-incubation with PACAP and NGF resulted in a synergistic up-regulation of serpinb1a expression (200-fold over control), suggesting that PACAP and NGF act through complementary mechanisms. Consistently, PACAP-induced serpinb1a expression was not blocked by TrkA receptor inhibition. Nevertheless, the stimulation of serpinb1a expression by PACAP and NGF was significantly reduced in the presence of extracellular signal-regulated kinase, calcineurin, protein kinase A, p38, and PI3K inhibitors, indicating that the two trophic factors share some common pathways in the regulation of serpinb1a. Finally, functional investigations conducted with siRNA revealed that serpinb1a is not involved in the effects of PACAP and NGF on PC12 cell neuritogenesis, proliferation or body cell volume but mediates their ability to block caspases 3/7 activity and to promote PC12 cell survival. Pituitary adenylate cyclase-activating polypeptide (PACAP) and nerve growth factor (NGF) induce a strong increase in serpinb1 a expression in PC12 cells. Functional investigations revealed that this increase in serpinb1a does not affect cell proliferation or differentiation but inhibits caspase 3 activity and promotes cell survival.-
dc.languageeng-
dc.publisherWiley-Blackwell Publishing Ltd. The Journal's web site is located at http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1471-4159-
dc.relation.ispartofJournal of Neurochemistry-
dc.titleInduction of serpinb1a by PACAP or NGF is required for PC12 cells survival after serum withdrawal-
dc.typeArticle-
dc.identifier.emailChow, BKC: bkcc@hku.hk-
dc.identifier.authorityChow, BKC=rp00681-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1111/jnc.12780-
dc.identifier.pmid24899316-
dc.identifier.pmcidPMC4177366-
dc.identifier.hkuros240049-
dc.identifier.volume131-
dc.identifier.issue1-
dc.identifier.spage21-
dc.identifier.epage32-
dc.identifier.isiWOS:000342849000004-
dc.publisher.placeUnited Kingdom-

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