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Article: Somatostatin-28 inhibitory action on somatolactin-α and -β gene expression in goldfish

TitleSomatostatin-28 inhibitory action on somatolactin-α and -β gene expression in goldfish
Authors
Issue Date2014
PublisherAmerican Physiological Society. The Journal's web site is located at http://intl-ajpregu.physiology.org
Citation
American Journal of Physiology: Regulatory, Integrative and Comparative Physiology, 2014, v. 307 n. 6, p. R755-R768 How to Cite?
AbstractSomatostain (SS) is known to inhibit growth hormone (GH) and prolactin (PRL) secretion. Somatolactin (SL) is a member of the GH/PRL family, but its regulation by goldfish brain somatostatin-28 (gbSS-28) has not been examined. To this end, the structural identity of goldfish SLα was established by 5'/3'-rapid amplification of cDNA ends. As revealed by in situ hybridization and immunohistochemical staining, the expression of SL isoforms was detected in pituitary cells located in the neurointermediate lobe (NIL). The transcripts of goldfish SS receptor 5a (Sst5a) but not Sst1b, Sst2, or Sst3a were detected in the goldfish NIL cells by RT-PCR. In goldfish pituitary cells, gbSS-28 not only had an inhibitory effect on basal SLα and SLβ mRNA levels but also could abolish insulin-like growth factor-stimulated SL gene expression. In primary cultures of goldfish NIL cells, gbSS-28 reduced forskolin-stimulated total cAMP production. With the use of a pharmacological approach, the adenylate cyclase (AC)/cAMP and phospholipase C (PLC)/inositol trisphosphate (IP3)/protein kinase C (PKC) cascades were shown to be involved in gbSS-28-inhibited SLα mRNA expression. Similar postreceptor signaling cascades were also observed for gbSS-28-reduced SLβ mRNA expression, except that PKC coupling to PLC was not involved. These results provide evidence that gbSS-28 can inhibit SLα and SLβ gene expression at the goldfish pituitary level via Sst5 through differential coupling of AC/cAMP and PLC/IP3/PKC cascades. Copyright © 2014 the American Physiological Society.
Persistent Identifierhttp://hdl.handle.net/10722/204825
ISSN
2015 Impact Factor: 3.168
2015 SCImago Journal Rankings: 1.663

 

DC FieldValueLanguage
dc.contributor.authorJiang, Q-
dc.contributor.authorWong, AOL-
dc.date.accessioned2014-09-20T00:42:48Z-
dc.date.available2014-09-20T00:42:48Z-
dc.date.issued2014-
dc.identifier.citationAmerican Journal of Physiology: Regulatory, Integrative and Comparative Physiology, 2014, v. 307 n. 6, p. R755-R768-
dc.identifier.issn0363-6119-
dc.identifier.urihttp://hdl.handle.net/10722/204825-
dc.description.abstractSomatostain (SS) is known to inhibit growth hormone (GH) and prolactin (PRL) secretion. Somatolactin (SL) is a member of the GH/PRL family, but its regulation by goldfish brain somatostatin-28 (gbSS-28) has not been examined. To this end, the structural identity of goldfish SLα was established by 5'/3'-rapid amplification of cDNA ends. As revealed by in situ hybridization and immunohistochemical staining, the expression of SL isoforms was detected in pituitary cells located in the neurointermediate lobe (NIL). The transcripts of goldfish SS receptor 5a (Sst5a) but not Sst1b, Sst2, or Sst3a were detected in the goldfish NIL cells by RT-PCR. In goldfish pituitary cells, gbSS-28 not only had an inhibitory effect on basal SLα and SLβ mRNA levels but also could abolish insulin-like growth factor-stimulated SL gene expression. In primary cultures of goldfish NIL cells, gbSS-28 reduced forskolin-stimulated total cAMP production. With the use of a pharmacological approach, the adenylate cyclase (AC)/cAMP and phospholipase C (PLC)/inositol trisphosphate (IP3)/protein kinase C (PKC) cascades were shown to be involved in gbSS-28-inhibited SLα mRNA expression. Similar postreceptor signaling cascades were also observed for gbSS-28-reduced SLβ mRNA expression, except that PKC coupling to PLC was not involved. These results provide evidence that gbSS-28 can inhibit SLα and SLβ gene expression at the goldfish pituitary level via Sst5 through differential coupling of AC/cAMP and PLC/IP3/PKC cascades. Copyright © 2014 the American Physiological Society.-
dc.languageeng-
dc.publisherAmerican Physiological Society. The Journal's web site is located at http://intl-ajpregu.physiology.org-
dc.relation.ispartofAmerican Journal of Physiology: Regulatory, Integrative and Comparative Physiology-
dc.rightsAmerican Journal of Physiology: Regulatory, Integrative and Comparative Physiology. Copyright © American Physiological Society.-
dc.titleSomatostatin-28 inhibitory action on somatolactin-α and -β gene expression in goldfish-
dc.typeArticle-
dc.identifier.emailWong, AOL: olwong@hkucc.hku.hk-
dc.identifier.authorityWong, AOL=rp00806-
dc.identifier.doi10.1152/ajpregu.00193.2014-
dc.identifier.pmid25009216-
dc.identifier.hkuros239582-
dc.identifier.hkuros253687-
dc.identifier.volume307-
dc.identifier.issue6-
dc.identifier.spageR755-
dc.identifier.epageR768-
dc.publisher.placeUnited States-

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