File Download

There are no files associated with this item.

Supplementary

Conference Paper: 5-HT modulates synaptic transmission in vestibular nucleus of rats

Title5-HT modulates synaptic transmission in vestibular nucleus of rats
Authors
KeywordsSerotonin
Neuromodulation
Vestibular
Issue Date2013
PublisherSociety for Neuroscience.
Citation
The 43rd Annual Meeting of the Society for Neuroscience (SfN), San Diego, California, USA, 9-13 November 2013 How to Cite?
AbstractSerotonergic projections from the raphe nucleus are known to innervate the medial vestibular nucleus (MV). Acute administration of serotonin (5-HT) to rat MV was found to prolong the execution time in balance beam test and in negative geotaxis, a behavioral indicator of gravity detection. To delineate the effect of 5-HT on neurotransmission within MV, whole-cell patch-clamp recording was conducted in brainstem slices of P14 rats. Both excitatory and inhibitory synaptic transmission were examined. In most MV neurons receiving glutamatergic input, 5-HT reduced the amplitude of evoked excitatory postsynaptic currents (eEPSC) and this effect was mimicked by 5-HT1A receptor agonist. On the other hand, 5-HT exerted differential effect on GABAA receptor-mediated inhibitory transmission within MV. In one subgroup of MV neurons, 5-HT suppressed the amplitude of evoked inhibitory postsynaptic currents (eIPSC) via 5-HT1A receptor. In another subgroup of MV neurons, 5-HT enhanced the amplitude of eIPSC. This effect could be mimicked by 5-HT2A receptor agonist but blocked by 5-HT2A receptor antagonist. With a paired-pulse stimulation paradigm, we found that all the aforementioned effects of 5-HT on either eEPSC or eIPSC were accompanied by change in paired-pulse ratio. This suggests that the 5-HT induced effects within the MV are mediated via the activation of presynaptic 5-HT receptors. Together, our results indicate that 5-HT plays a heterogeneous role in modulating excitatory and inhibitory transmission within the MV circuitry, thereby contributing to vestibular-related behavioral outcome.
DescriptionPoster session 798: Serotonin Receptors
Program#798.12 & Poster#C26
Fulltext of the abstract in: http://www.abstractsonline.com/Plan/ViewAbstract.aspx?sKey=c6cfcb31-43c1-4e5f-b7f1-888d363f5f74&cKey=29f2021d-1a95-4bfd-aef1-beaacc1dfce4&mKey=8d2a5bec-4825-4cd6-9439-b42bb151d1cf
Persistent Identifierhttp://hdl.handle.net/10722/204407

 

DC FieldValueLanguage
dc.contributor.authorHan, Len_US
dc.contributor.authorLai, SKen_US
dc.contributor.authorLai, CHen_US
dc.contributor.authorChan, YSen_US
dc.date.accessioned2014-09-19T23:34:42Z-
dc.date.available2014-09-19T23:34:42Z-
dc.date.issued2013en_US
dc.identifier.citationThe 43rd Annual Meeting of the Society for Neuroscience (SfN), San Diego, California, USA, 9-13 November 2013en_US
dc.identifier.urihttp://hdl.handle.net/10722/204407-
dc.descriptionPoster session 798: Serotonin Receptors-
dc.descriptionProgram#798.12 & Poster#C26-
dc.descriptionFulltext of the abstract in: http://www.abstractsonline.com/Plan/ViewAbstract.aspx?sKey=c6cfcb31-43c1-4e5f-b7f1-888d363f5f74&cKey=29f2021d-1a95-4bfd-aef1-beaacc1dfce4&mKey=8d2a5bec-4825-4cd6-9439-b42bb151d1cf-
dc.description.abstractSerotonergic projections from the raphe nucleus are known to innervate the medial vestibular nucleus (MV). Acute administration of serotonin (5-HT) to rat MV was found to prolong the execution time in balance beam test and in negative geotaxis, a behavioral indicator of gravity detection. To delineate the effect of 5-HT on neurotransmission within MV, whole-cell patch-clamp recording was conducted in brainstem slices of P14 rats. Both excitatory and inhibitory synaptic transmission were examined. In most MV neurons receiving glutamatergic input, 5-HT reduced the amplitude of evoked excitatory postsynaptic currents (eEPSC) and this effect was mimicked by 5-HT1A receptor agonist. On the other hand, 5-HT exerted differential effect on GABAA receptor-mediated inhibitory transmission within MV. In one subgroup of MV neurons, 5-HT suppressed the amplitude of evoked inhibitory postsynaptic currents (eIPSC) via 5-HT1A receptor. In another subgroup of MV neurons, 5-HT enhanced the amplitude of eIPSC. This effect could be mimicked by 5-HT2A receptor agonist but blocked by 5-HT2A receptor antagonist. With a paired-pulse stimulation paradigm, we found that all the aforementioned effects of 5-HT on either eEPSC or eIPSC were accompanied by change in paired-pulse ratio. This suggests that the 5-HT induced effects within the MV are mediated via the activation of presynaptic 5-HT receptors. Together, our results indicate that 5-HT plays a heterogeneous role in modulating excitatory and inhibitory transmission within the MV circuitry, thereby contributing to vestibular-related behavioral outcome.-
dc.languageengen_US
dc.publisherSociety for Neuroscience.-
dc.relation.ispartofNeuroscience 2013en_US
dc.rightsNeuroscience 2013. Copyright © Society for Neuroscience.-
dc.subjectSerotonin-
dc.subjectNeuromodulation-
dc.subjectVestibular-
dc.title5-HT modulates synaptic transmission in vestibular nucleus of ratsen_US
dc.typeConference_Paperen_US
dc.identifier.emailHan, L: rahanlei@hku.hken_US
dc.identifier.emailLai, SK: estherlai@hkusua.hku.hken_US
dc.identifier.emailLai, CH: chlaib@hku.hken_US
dc.identifier.emailChan, YS: yschan@hku.hken_US
dc.identifier.authorityLai, CH=rp00396en_US
dc.identifier.authorityChan, YS=rp00318en_US
dc.identifier.hkuros238211en_US
dc.publisher.placeUnited States-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats