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Conference Paper: Protein overload induces renal proximal tubular epithelial cell apoptosis by down-regulating WNT/β-catenin signalling

TitleProtein overload induces renal proximal tubular epithelial cell apoptosis by down-regulating WNT/β-catenin signalling
Authors
Issue Date2013
PublisherAmerican Society of Nephrology. The Journal's web site is located at https://www.asn-online.org/abstracts/
Citation
Kidney Week 2013, Atlanta, GA., 5-10 November 2013. In Journal of the American Society of Nephrology, 2013, v. 24, p. 409A, abstract no. FR-PO196 How to Cite?
AbstractBACKGROUND: Numerous studies have demonstrated a tubulotoxic role of excess proteins on renal proximal tubular epithelial cell (PTECs) via various signalling pathways. However, the role of Wnt/β-catenin signalling in PTECs during protein overload remains unknown. METHODS: Wnt/β-catenin expressions were measured in control and human serum albumin (HSA)-treated human kidney 2 (HK-2) cells by real-time PCR and Western blotting. Genetic knockdown of β-catenin was achieved using siRNA transfection. Apoptotic phenotypes were evaluated by real-time PCR and TUNEL assay. RESULTS: Upon the 4-day-HSA stimulation, gene expression of β-catenin, frizzle-dreceptor 1 and Wnt-1 in PTECs declined by 26%±2 (p<0.05, t-test), 65%±2 (p<0.05) and 57%±6 (p<0.05) versus control, respectively. Western blots showed that protein expression of cytosolic and nuclear active β-catenin decreased by 57%±8 (p<0.05) and 66%±8 (p<0.05) after 4-day HAS treatment, respectively. Simultaneously, Bax/Bcl-2 gene expression ratio increased by 31%±8 (p<0.05). Transfection of β-catenin siRNA into HK-2 cells up-regulated Bax/Bcl-2 gene expression ratio by 23%±7 (p<0.05) relative to mock transfection. HAS treatment and β-catenin siRNA transfection increased the number of TUNEL-positive cells by 70%±10 (p<0.05) and 73%±8 (p<0.05), respectively. CONCLUSIONS: Protein-overload promotes tubular cell apoptosis via down-regulation of Wnt/β-catenin signalling in PTECs.
DescriptionFriday Poster Presentation - Cell Signaling in Kidney Fibrosis - II: no. FR-PO196
Persistent Identifierhttp://hdl.handle.net/10722/204260
ISSN
2015 Impact Factor: 8.491
2015 SCImago Journal Rankings: 4.699

 

DC FieldValueLanguage
dc.contributor.authorWong, DWLen_US
dc.contributor.authorYiu, WHen_US
dc.contributor.authorWu, Hen_US
dc.contributor.authorLi, Ren_US
dc.contributor.authorLeung, JCKen_US
dc.contributor.authorChan, LYYen_US
dc.contributor.authorLai, KNen_US
dc.contributor.authorTang, SCWen_US
dc.date.accessioned2014-09-19T21:43:10Z-
dc.date.available2014-09-19T21:43:10Z-
dc.date.issued2013en_US
dc.identifier.citationKidney Week 2013, Atlanta, GA., 5-10 November 2013. In Journal of the American Society of Nephrology, 2013, v. 24, p. 409A, abstract no. FR-PO196en_US
dc.identifier.issn1046-6673-
dc.identifier.urihttp://hdl.handle.net/10722/204260-
dc.descriptionFriday Poster Presentation - Cell Signaling in Kidney Fibrosis - II: no. FR-PO196-
dc.description.abstractBACKGROUND: Numerous studies have demonstrated a tubulotoxic role of excess proteins on renal proximal tubular epithelial cell (PTECs) via various signalling pathways. However, the role of Wnt/β-catenin signalling in PTECs during protein overload remains unknown. METHODS: Wnt/β-catenin expressions were measured in control and human serum albumin (HSA)-treated human kidney 2 (HK-2) cells by real-time PCR and Western blotting. Genetic knockdown of β-catenin was achieved using siRNA transfection. Apoptotic phenotypes were evaluated by real-time PCR and TUNEL assay. RESULTS: Upon the 4-day-HSA stimulation, gene expression of β-catenin, frizzle-dreceptor 1 and Wnt-1 in PTECs declined by 26%±2 (p<0.05, t-test), 65%±2 (p<0.05) and 57%±6 (p<0.05) versus control, respectively. Western blots showed that protein expression of cytosolic and nuclear active β-catenin decreased by 57%±8 (p<0.05) and 66%±8 (p<0.05) after 4-day HAS treatment, respectively. Simultaneously, Bax/Bcl-2 gene expression ratio increased by 31%±8 (p<0.05). Transfection of β-catenin siRNA into HK-2 cells up-regulated Bax/Bcl-2 gene expression ratio by 23%±7 (p<0.05) relative to mock transfection. HAS treatment and β-catenin siRNA transfection increased the number of TUNEL-positive cells by 70%±10 (p<0.05) and 73%±8 (p<0.05), respectively. CONCLUSIONS: Protein-overload promotes tubular cell apoptosis via down-regulation of Wnt/β-catenin signalling in PTECs.-
dc.languageengen_US
dc.publisherAmerican Society of Nephrology. The Journal's web site is located at https://www.asn-online.org/abstracts/-
dc.relation.ispartofJASN Abstract Supplementen_US
dc.titleProtein overload induces renal proximal tubular epithelial cell apoptosis by down-regulating WNT/β-catenin signallingen_US
dc.typeConference_Paperen_US
dc.identifier.emailYiu, WH: whyiu@hku.hken_US
dc.identifier.emailWu, H: hjwu@hku.hken_US
dc.identifier.emailLeung, JCK: jckleung@hku.hken_US
dc.identifier.emailChan, LYY: yychanb@hku.hken_US
dc.identifier.emailLai, KN: knlai@hku.hken_US
dc.identifier.emailTang, SCW: scwtang@hku.hken_US
dc.identifier.authorityLeung, JCK=rp00448en_US
dc.identifier.authorityLai, KN=rp00324en_US
dc.identifier.authorityTang, SCW=rp00480en_US
dc.description.naturelink_to_OA_fulltext-
dc.identifier.hkuros235360en_US
dc.identifier.volume24-
dc.identifier.spage409A, abstract no. FR-PO196-
dc.identifier.epage409A, abstract no. FR-PO196-
dc.publisher.placeUnited States-

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