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Conference Paper: Dosage-dependent requirement of sedlin component of the TRAPP complex in mouse embryonic development

TitleDosage-dependent requirement of sedlin component of the TRAPP complex in mouse embryonic development
Authors
Issue Date2013
Citation
The 9th Pan Pacific Connective Tissue Societies Symposium (PPCTSS 2013), Hong Kong, China, 24-27 November 2013. In the Program Book of the 9th Pan Pacific Connective Tissue Societies Symposium, 2013, p. abstract no. 0049 How to Cite?
AbstractIntracellular vesicle trafficking is essential for proper cellular function and mammalian development. Transport Protein Particle (TRAPP) complex regulates multiple vesicles transport pathways. Three TRAPP complexes have been identified in yeast with roles in ER-Golgi, post-Golgi transport and in autophagy. Sedlin (SEDL) is one of the subunit of the TRAPP complex and mutations in human SEDL gene are responsible for an X-linked recessive skeletal disorder called Spondyloepiphyseal Dysplasia Tarda (SEDT). The SEDL gene is ubiquitously expressed, however the underlying defect is only restricted to derangement of chondrogenesis reflecting the existence of another SEDL-related gene. We use gene-targeting and gene-trap mutagenesis strategies to investigate the functions of Sedl and Sedlp in mice. Our results show that Sedl (Sedlin) and Sedlp have redundant function and are essentially required for mouse development. We showed here that there is a dose-dependent requirement of Sedl and Sedlp in early embryonic development. Embryos with a reduced dosage of Sedl and Sedlp display a significantly reduced size with severe abnormalities and a disorganized yolk-sac endoderm structure. Electron microscopy study indicates morphological alterations of the intracellular compartments in compound mutant endoderm suggesting both Sedlin and Sedlp may be required for proper cellular trafficking. These results provide important insights into the functional roles of Sedlin components in mouse embryonic development.
DescriptionConference Theme: The Extracellular Matrix Niche
Poster Presentation
The Program can be viewed at: http://ppctss2013.org/Online%20Program%20Book.pdf
Persistent Identifierhttp://hdl.handle.net/10722/203819

 

DC FieldValueLanguage
dc.contributor.authorChan, CCYen_US
dc.contributor.authorChan, Den_US
dc.contributor.authorTanner, JAen_US
dc.contributor.authorLuk, KDKen_US
dc.contributor.authorCheah, KSEen_US
dc.date.accessioned2014-09-19T16:41:13Z-
dc.date.available2014-09-19T16:41:13Z-
dc.date.issued2013en_US
dc.identifier.citationThe 9th Pan Pacific Connective Tissue Societies Symposium (PPCTSS 2013), Hong Kong, China, 24-27 November 2013. In the Program Book of the 9th Pan Pacific Connective Tissue Societies Symposium, 2013, p. abstract no. 0049en_US
dc.identifier.urihttp://hdl.handle.net/10722/203819-
dc.descriptionConference Theme: The Extracellular Matrix Niche-
dc.descriptionPoster Presentation-
dc.descriptionThe Program can be viewed at: http://ppctss2013.org/Online%20Program%20Book.pdf-
dc.description.abstractIntracellular vesicle trafficking is essential for proper cellular function and mammalian development. Transport Protein Particle (TRAPP) complex regulates multiple vesicles transport pathways. Three TRAPP complexes have been identified in yeast with roles in ER-Golgi, post-Golgi transport and in autophagy. Sedlin (SEDL) is one of the subunit of the TRAPP complex and mutations in human SEDL gene are responsible for an X-linked recessive skeletal disorder called Spondyloepiphyseal Dysplasia Tarda (SEDT). The SEDL gene is ubiquitously expressed, however the underlying defect is only restricted to derangement of chondrogenesis reflecting the existence of another SEDL-related gene. We use gene-targeting and gene-trap mutagenesis strategies to investigate the functions of Sedl and Sedlp in mice. Our results show that Sedl (Sedlin) and Sedlp have redundant function and are essentially required for mouse development. We showed here that there is a dose-dependent requirement of Sedl and Sedlp in early embryonic development. Embryos with a reduced dosage of Sedl and Sedlp display a significantly reduced size with severe abnormalities and a disorganized yolk-sac endoderm structure. Electron microscopy study indicates morphological alterations of the intracellular compartments in compound mutant endoderm suggesting both Sedlin and Sedlp may be required for proper cellular trafficking. These results provide important insights into the functional roles of Sedlin components in mouse embryonic development.-
dc.languageengen_US
dc.relation.ispartofPan Pacific Connective Tissue Societies Symposium (PPCTSS)en_US
dc.titleDosage-dependent requirement of sedlin component of the TRAPP complex in mouse embryonic developmenten_US
dc.typeConference_Paperen_US
dc.identifier.emailChan, CCY: calebccy@hku.hken_US
dc.identifier.emailChan, D: chand@hku.hken_US
dc.identifier.emailTanner, JA: jatanner@hku.hken_US
dc.identifier.emailLuk, KDK: hrmoldk@hkucc.hku.hken_US
dc.identifier.emailCheah, KSE: hrmbdkc@hku.hken_US
dc.identifier.authorityChan, D=rp00540en_US
dc.identifier.authorityTanner, JA=rp00495en_US
dc.identifier.authorityLuk, KDK=rp00333en_US
dc.identifier.hkuros238508en_US

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