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Conference Paper: The regulation of motor neuron migration by differential expression of chondroitin sulfotransferase in rat hindbrain development

TitleThe regulation of motor neuron migration by differential expression of chondroitin sulfotransferase in rat hindbrain development
Authors
Issue Date2013
Citation
The 9th Pan Pacific Connective Tissue Societies Symposium, Hong Kong, China, 24-27 November 2013. In the Proceedings of the 9th Pan Pacific Connective Tissue Societies Symposium, 2013 How to Cite?
AbstractChondroitin sulfate (CS) moieties of proteoglycans are extracellular matrix components that have been implicated in the timing and patterning of axon fasciculation during embryonic brain development. It, nonetheless, remains unclear if these moieties can also control neuronal soma migration in the developmental process. We hypothesized that the cranial motor neuron migration in the hindbrain is modulated by varying sulfation patterns of the chondroitins between the migrating and ready-to-migrate neurons. In this project, hindbrain explants of E11.5 Sprague Dawley rats were maintained in culture. In control cultures, time lapse video microscopy revealed advancement of neuronal cell bodies in the direction of the leading process away from the explant core. In test cultures treated with chondroitinase ABC, the neuronal cell bodies lost the directional movement but not the motility. Immunocytochemistry confirmed the presence of CS56 epitopes among Tuj-1-positive neurons not only in the explant core and those advancing beyond the core, but also in the environment surrounding the migrating neuronal cell bodies. In situ hybridization revealed the relatively abundant expression of chondroitin-4-sulfotransferase 2 (C4ST2) mRNA among cells heading away from the explant core. Taken together, the present results showed that CS moieties expressed by migrating neurons differ in sulfation pattern from those in the vicinity of non-migrating neurons.
DescriptionConference Theme: The extracellular matrix niche
Session 1: The Matrix niche
Persistent Identifierhttp://hdl.handle.net/10722/203794

 

DC FieldValueLanguage
dc.contributor.authorWong, MKen_US
dc.contributor.authorLi, Men_US
dc.contributor.authorChan, YSen_US
dc.contributor.authorShum, DKYen_US
dc.date.accessioned2014-09-19T16:41:05Z-
dc.date.available2014-09-19T16:41:05Z-
dc.date.issued2013en_US
dc.identifier.citationThe 9th Pan Pacific Connective Tissue Societies Symposium, Hong Kong, China, 24-27 November 2013. In the Proceedings of the 9th Pan Pacific Connective Tissue Societies Symposium, 2013en_US
dc.identifier.urihttp://hdl.handle.net/10722/203794-
dc.descriptionConference Theme: The extracellular matrix niche-
dc.descriptionSession 1: The Matrix niche-
dc.description.abstractChondroitin sulfate (CS) moieties of proteoglycans are extracellular matrix components that have been implicated in the timing and patterning of axon fasciculation during embryonic brain development. It, nonetheless, remains unclear if these moieties can also control neuronal soma migration in the developmental process. We hypothesized that the cranial motor neuron migration in the hindbrain is modulated by varying sulfation patterns of the chondroitins between the migrating and ready-to-migrate neurons. In this project, hindbrain explants of E11.5 Sprague Dawley rats were maintained in culture. In control cultures, time lapse video microscopy revealed advancement of neuronal cell bodies in the direction of the leading process away from the explant core. In test cultures treated with chondroitinase ABC, the neuronal cell bodies lost the directional movement but not the motility. Immunocytochemistry confirmed the presence of CS56 epitopes among Tuj-1-positive neurons not only in the explant core and those advancing beyond the core, but also in the environment surrounding the migrating neuronal cell bodies. In situ hybridization revealed the relatively abundant expression of chondroitin-4-sulfotransferase 2 (C4ST2) mRNA among cells heading away from the explant core. Taken together, the present results showed that CS moieties expressed by migrating neurons differ in sulfation pattern from those in the vicinity of non-migrating neurons.-
dc.languageengen_US
dc.relation.ispartofProceedings of the 9th Pan Pacific Connective Tissue Societies Symposiumen_US
dc.titleThe regulation of motor neuron migration by differential expression of chondroitin sulfotransferase in rat hindbrain developmenten_US
dc.typeConference_Paperen_US
dc.identifier.emailWong, MK: wongmmk@hku.hken_US
dc.identifier.emailLi, M: limeihk@hku.hken_US
dc.identifier.emailChan, YS: yschan@hku.hken_US
dc.identifier.emailShum, DKY: shumdkhk@hkucc.hku.hken_US
dc.identifier.authorityChan, YS=rp00318en_US
dc.identifier.authorityShum, DKY=rp00321en_US
dc.identifier.hkuros238216en_US

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