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Conference Paper: Notch Signaling in in vitro derivation of Schwann cells from bone marrow stromal cells

TitleNotch Signaling in in vitro derivation of Schwann cells from bone marrow stromal cells
Authors
Issue Date2013
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/37090
Citation
The 11th European Meeting on Glial Cells in Health and Disease (GLIA Meeting 2013), Berlin, Germany, 3-6 July 2013. In Glia, 2013, v. 61 suppl. S1, p. S55, abstract no. T01-12B How to Cite?
AbstractBone marrow stromal cells can be an autologous source of Schwann cells for transplantation to treat demyelination related diseases. Our strategy of deriving Schwann cells from bone marrow stromal cells (Shea et.al, 2010) exploited dorsal root ganglia (DRG) neurons to provide Schwann cell-like cells with juxtacrine signals that mediated commitment to the Schwann cell fate. To address if the juxtacrine signaling is served by Notch and its ligands, analysis of the DRG neurons found cell surface immunopositivities for the ligands, DLL1 and Jagged1 whereas that of bone marrow-derived Schwann cell-like cells found immunopositivity for the receptor, Notch-1. In cocultures with DRG neurons, during which Schwann cell-like cells underwent changes towards fate commitment, the nuclear localization of Notch intracellular domain (NICD) and the expression of downstream target gene hes1 by PCR, were indicative of Notch signaling. This was simultaneous with progressive increase in ErbB2/B3 expression as revealed by immunocytochemistry and Western blotting. Taken together, Notch signaling between DRG neuron and Schwann cell-like cells in contact mediated the upregulation of ErbB receptors in Schwann cell-like cells for signaling interaction with neuregulin as they transition into fate commitment.
DescriptionPoster Session 2 - Topic: T1 Cell proliferation, lineages and differentiation: no. T01-12B
This journal suppl. is Abstract Book of the Meeting
Online abstract search: http://berlin2013.gliameeting.eu/program
Persistent Identifierhttp://hdl.handle.net/10722/203742
ISSN
2015 Impact Factor: 5.997
2015 SCImago Journal Rankings: 3.296

 

DC FieldValueLanguage
dc.contributor.authorTai, EWYen_US
dc.contributor.authorShea, GKHen_US
dc.contributor.authorTsui, AYPen_US
dc.contributor.authorLeung, KHYen_US
dc.contributor.authorChan, YSen_US
dc.contributor.authorShum, DKY-
dc.date.accessioned2014-09-19T16:39:55Z-
dc.date.available2014-09-19T16:39:55Z-
dc.date.issued2013en_US
dc.identifier.citationThe 11th European Meeting on Glial Cells in Health and Disease (GLIA Meeting 2013), Berlin, Germany, 3-6 July 2013. In Glia, 2013, v. 61 suppl. S1, p. S55, abstract no. T01-12Ben_US
dc.identifier.issn0894-1491-
dc.identifier.urihttp://hdl.handle.net/10722/203742-
dc.descriptionPoster Session 2 - Topic: T1 Cell proliferation, lineages and differentiation: no. T01-12B-
dc.descriptionThis journal suppl. is Abstract Book of the Meeting-
dc.descriptionOnline abstract search: http://berlin2013.gliameeting.eu/program-
dc.description.abstractBone marrow stromal cells can be an autologous source of Schwann cells for transplantation to treat demyelination related diseases. Our strategy of deriving Schwann cells from bone marrow stromal cells (Shea et.al, 2010) exploited dorsal root ganglia (DRG) neurons to provide Schwann cell-like cells with juxtacrine signals that mediated commitment to the Schwann cell fate. To address if the juxtacrine signaling is served by Notch and its ligands, analysis of the DRG neurons found cell surface immunopositivities for the ligands, DLL1 and Jagged1 whereas that of bone marrow-derived Schwann cell-like cells found immunopositivity for the receptor, Notch-1. In cocultures with DRG neurons, during which Schwann cell-like cells underwent changes towards fate commitment, the nuclear localization of Notch intracellular domain (NICD) and the expression of downstream target gene hes1 by PCR, were indicative of Notch signaling. This was simultaneous with progressive increase in ErbB2/B3 expression as revealed by immunocytochemistry and Western blotting. Taken together, Notch signaling between DRG neuron and Schwann cell-like cells in contact mediated the upregulation of ErbB receptors in Schwann cell-like cells for signaling interaction with neuregulin as they transition into fate commitment.en_US
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/37090-
dc.relation.ispartofGliaen_US
dc.rightsGlia. Copyright © John Wiley & Sons, Inc.-
dc.titleNotch Signaling in in vitro derivation of Schwann cells from bone marrow stromal cellsen_US
dc.typeConference_Paperen_US
dc.identifier.emailShea, GKH: gkshea@hku.hken_US
dc.identifier.emailTsui, AYP: alex2013@hku.hken_US
dc.identifier.emailChan, YS: yschan@hku.hken_US
dc.identifier.emailShum, DKY: shumdkhk@hkucc.hku.hken_US
dc.identifier.authorityShea, GKH=rp01781en_US
dc.identifier.authorityChan, YS=rp00318en_US
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1002/glia.22530-
dc.identifier.hkuros235261en_US
dc.identifier.hkuros238146-
dc.identifier.volume61-
dc.identifier.issuesuppl. S1-
dc.identifier.spageS55, abstract no. T01-12B-
dc.identifier.epageS55, abstract no. T01-12B-
dc.publisher.placeUnited States-

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