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Article: Stringent requirement for spatial arrangement of extracellular matrix in supporting cell morphogenesis and differentiation

TitleStringent requirement for spatial arrangement of extracellular matrix in supporting cell morphogenesis and differentiation
Authors
Issue Date2014
PublisherBioMed Central Ltd. The Journal's web site is located at http://www.biomedcentral.com/bmccellbiol/
Citation
BMC Cell Biology, 2014, v. 15, article no. 10 How to Cite?
AbstractBACKGROUND: In vitro experiments on the functional roles of extracellular matrix (ECM) components usually involve the culture of cells on surfaces coated with purified ECM components. These experiments can seldom recuperate the spatial arrangement of ECM found in vivo. In this study, we have overcome this obstacle by using histological sections of bovine Achilles tendon as cell culture substrates. RESULTS: We found that tendon sections can be viewed as a pre-formed block of ECM in which the collagen fibrils exhibited a spatial regularity unraveled in any artificially constructed scaffold. By carving the tendon at different angles relative to its main axis, we created different surfaces with distinct spatial arrangements of collagen fibrils. To assess the cellular responses to these surfaces, human mesenchymal stem cells (MSCs) were directly cultured on these sections, hence exposed to the collagen with different spatial orientations. Cells seeded on longitudinal tendon sections adopted a highly elongated and aligned morphology, and expressed an increased level of tenomodulin, suggesting that the collagen fibrils present in this section provide a microenvironment that facilitates cell morphogenesis and differentiation. However, MSC elongation, alignment and induction of tenomodulin diminished dramatically even as the sectioned angle changed slightly. CONCLUSION: Our results suggest that cell functions are influenced not only by the type or concentration of ECM components, but also by the precise spatial arrangements of these molecules. The method developed in this study offers a simple and robust way for the studying of cell-ECM interactions, and opens many research avenues in the field of matrix biology.
Persistent Identifierhttp://hdl.handle.net/10722/203233
ISSN
2015 Impact Factor: 2.584
2015 SCImago Journal Rankings: 1.486
PubMed Central ID

 

DC FieldValueLanguage
dc.contributor.authorTang, SWen_US
dc.contributor.authorTong, WYTen_US
dc.contributor.authorShen, Wen_US
dc.contributor.authorYeung, KWKen_US
dc.contributor.authorLam, YWen_US
dc.date.accessioned2014-09-19T13:11:02Z-
dc.date.available2014-09-19T13:11:02Z-
dc.date.issued2014en_US
dc.identifier.citationBMC Cell Biology, 2014, v. 15, article no. 10en_US
dc.identifier.issn1471-2121-
dc.identifier.urihttp://hdl.handle.net/10722/203233-
dc.description.abstractBACKGROUND: In vitro experiments on the functional roles of extracellular matrix (ECM) components usually involve the culture of cells on surfaces coated with purified ECM components. These experiments can seldom recuperate the spatial arrangement of ECM found in vivo. In this study, we have overcome this obstacle by using histological sections of bovine Achilles tendon as cell culture substrates. RESULTS: We found that tendon sections can be viewed as a pre-formed block of ECM in which the collagen fibrils exhibited a spatial regularity unraveled in any artificially constructed scaffold. By carving the tendon at different angles relative to its main axis, we created different surfaces with distinct spatial arrangements of collagen fibrils. To assess the cellular responses to these surfaces, human mesenchymal stem cells (MSCs) were directly cultured on these sections, hence exposed to the collagen with different spatial orientations. Cells seeded on longitudinal tendon sections adopted a highly elongated and aligned morphology, and expressed an increased level of tenomodulin, suggesting that the collagen fibrils present in this section provide a microenvironment that facilitates cell morphogenesis and differentiation. However, MSC elongation, alignment and induction of tenomodulin diminished dramatically even as the sectioned angle changed slightly. CONCLUSION: Our results suggest that cell functions are influenced not only by the type or concentration of ECM components, but also by the precise spatial arrangements of these molecules. The method developed in this study offers a simple and robust way for the studying of cell-ECM interactions, and opens many research avenues in the field of matrix biology.-
dc.languageengen_US
dc.publisherBioMed Central Ltd. The Journal's web site is located at http://www.biomedcentral.com/bmccellbiol/-
dc.relation.ispartofBMC Cell Biologyen_US
dc.rightsBMC Cell Biology. Copyright © BioMed Central Ltd.-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.meshCell Differentiation-
dc.subject.meshExtracellular Matrix - chemistry - metabolism-
dc.subject.meshMesenchymal Stromal Cells - cytology - metabolism-
dc.subject.meshMicroscopy, Electron, Scanning-
dc.subject.meshTendons - chemistry - pathology-
dc.titleStringent requirement for spatial arrangement of extracellular matrix in supporting cell morphogenesis and differentiationen_US
dc.typeArticleen_US
dc.identifier.emailYeung, KWK: wkkyeung@hku.hken_US
dc.identifier.authorityYeung, KWK=rp00309en_US
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1186/1471-2121-15-10-
dc.identifier.pmid24661496-
dc.identifier.pmcidPMC3987840-
dc.identifier.hkuros237707en_US
dc.identifier.volume15en_US
dc.publisher.placeUnited Kingdom-

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