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Article: Delivering mesenchymal stem cells in collagen microsphere carriers to rabbit degenerative disc - Reduced risk of osteophyte formation.

TitleDelivering mesenchymal stem cells in collagen microsphere carriers to rabbit degenerative disc - Reduced risk of osteophyte formation.
Authors
Issue Date2014
PublisherMary Ann Liebert, Inc. Publishers. The Journal's web site is located at http://www.liebertpub.com/publication.aspx?pub_id=263
Citation
Tissue Engineering Part A: Tissue Engineering, 2014, v. 20 n. 9-10, p. 1379-1391 How to Cite?
AbstractMesenchymal stem cells (MSCs) have the potential to treat early intervertebral disc (IVD) degeneration. However, during intradiscal injection, the vast majority of cells leaked out even in the presence of hydrogel carrier. Recent evidence suggests that annulus puncture is associated with cell leakage and contributes to osteophyte formation, an undesirable side effect. This suggests the significance of developing appropriate carriers for intradiscal delivery of MSCs. We previously developed a collagen microencapsulation platform, which entraps MSCs in a solid microsphere consisting of collagen nanofiber meshwork. These solid yet porous microspheres support MSC attachment, survival, proliferation, migration, differentiation, and matrix remodeling. Here we hypothesize that intradiscal injection of MSCs in collagen microspheres will outperform that of MSCs in saline in terms of better functional outcomes and reduced side effects. Specifically, we induced disc degeneration in rabbits and then intradiscally injected autologous MSCs, either packaged within collagen microspheres or directly suspended in saline, into different disc levels. Functional outcomes including hydration index and disc height were monitored regularly until 6 months. Upon sacrifice, the involved discs were harvested for histological, biochemical, and biomechanical evaluations. MSCs in collagen microspheres showed advantage over MSCs in saline in better maintaining the dynamic mechanical behavior but similar performance in hydration and disc height maintenance and matrix composition. More importantly, upon examination of gross appearance, radiograph, and histology of IVD, delivering MSCs in collagen microspheres significantly reduced the risk of osteophyte formation as compared to that in saline. This work demonstrates the significance of using cell carriers during intradiscal injection of MSCs in treating disc degeneration.
Persistent Identifierhttp://hdl.handle.net/10722/203038
ISSN
2015 SCImago Journal Rankings: 1.500
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLi, YY-
dc.contributor.authorDiao, HJ-
dc.contributor.authorChik, TK-
dc.contributor.authorChow, ST-
dc.contributor.authorAn, XM-
dc.contributor.authorLeung, VYL-
dc.contributor.authorCheung, KMC-
dc.contributor.authorChan, BP-
dc.date.accessioned2014-09-19T11:08:20Z-
dc.date.available2014-09-19T11:08:20Z-
dc.date.issued2014-
dc.identifier.citationTissue Engineering Part A: Tissue Engineering, 2014, v. 20 n. 9-10, p. 1379-1391-
dc.identifier.issn1937-3341-
dc.identifier.urihttp://hdl.handle.net/10722/203038-
dc.description.abstractMesenchymal stem cells (MSCs) have the potential to treat early intervertebral disc (IVD) degeneration. However, during intradiscal injection, the vast majority of cells leaked out even in the presence of hydrogel carrier. Recent evidence suggests that annulus puncture is associated with cell leakage and contributes to osteophyte formation, an undesirable side effect. This suggests the significance of developing appropriate carriers for intradiscal delivery of MSCs. We previously developed a collagen microencapsulation platform, which entraps MSCs in a solid microsphere consisting of collagen nanofiber meshwork. These solid yet porous microspheres support MSC attachment, survival, proliferation, migration, differentiation, and matrix remodeling. Here we hypothesize that intradiscal injection of MSCs in collagen microspheres will outperform that of MSCs in saline in terms of better functional outcomes and reduced side effects. Specifically, we induced disc degeneration in rabbits and then intradiscally injected autologous MSCs, either packaged within collagen microspheres or directly suspended in saline, into different disc levels. Functional outcomes including hydration index and disc height were monitored regularly until 6 months. Upon sacrifice, the involved discs were harvested for histological, biochemical, and biomechanical evaluations. MSCs in collagen microspheres showed advantage over MSCs in saline in better maintaining the dynamic mechanical behavior but similar performance in hydration and disc height maintenance and matrix composition. More importantly, upon examination of gross appearance, radiograph, and histology of IVD, delivering MSCs in collagen microspheres significantly reduced the risk of osteophyte formation as compared to that in saline. This work demonstrates the significance of using cell carriers during intradiscal injection of MSCs in treating disc degeneration.-
dc.languageeng-
dc.publisherMary Ann Liebert, Inc. Publishers. The Journal's web site is located at http://www.liebertpub.com/publication.aspx?pub_id=263-
dc.relation.ispartofTissue Engineering Part A: Tissue Engineering-
dc.rightsThis is a copy of an article published in the Tissue Engineering Part A: Tissue Engineering © 2014 copyright Mary Ann Liebert, Inc.;Tissue Engineering Part A: Tissue Engineering is available online at: http://www.liebertonline.com.-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.titleDelivering mesenchymal stem cells in collagen microsphere carriers to rabbit degenerative disc - Reduced risk of osteophyte formation.-
dc.typeArticle-
dc.identifier.emailLi, YY: cyyli@graduate.hku.hk-
dc.identifier.emailDiao, HJ: huajia@hku.hk-
dc.identifier.emailChik, TK: tkchik@hku.hk-
dc.identifier.emailChow, ST: chowst@hku.hk-
dc.identifier.emailAn, XM: anxmhk@hku.hk-
dc.identifier.emailLeung, VYL: vicleung@hku.hk-
dc.identifier.emailCheung, KMC: cheungmc@hku.hk-
dc.identifier.emailChan, BP: bpchan@hkucc.hku.hk-
dc.identifier.authorityLeung, VYL=rp01764-
dc.identifier.authorityCheung, KMC=rp00387-
dc.identifier.authorityChan, BP=rp00087-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1089/ten.TEA.2013.0498-
dc.identifier.pmid24372278-
dc.identifier.pmcidPMC4011461-
dc.identifier.scopuseid_2-s2.0-84899830821-
dc.identifier.hkuros239627-
dc.identifier.volume20-
dc.identifier.issue9-10-
dc.identifier.spage1379-
dc.identifier.epage1391-
dc.identifier.isiWOS:000335661400005-
dc.publisher.placeUnited States-

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