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Article: Mesenchymal stem cell-collagen microspheres for articular cartilage repair: cell density and differentiation status.

TitleMesenchymal stem cell-collagen microspheres for articular cartilage repair: cell density and differentiation status.
Authors
Issue Date2014
PublisherElsevier. The Journal's web site is located at http://www.elsevier.com/locate/actabiomat
Citation
Acta Biomaterialia, 2014, v. 10, Issue 5, p. 1919-1929 How to Cite?
AbstractMesenchymal stem cells (MSC) hold promise for cartilage repair. A microencapsulation technique was previously established to entrap MSC in collagen microspheres, and the collagen fibrous meshwork was found to be an excellent scaffold for supporting MSC survival, growth and differentiation. This study investigates the importance of cell density and differentiation status of MSC-collagen microspheres in cartilage repair. MSC were isolated from rabbit bone marrow and encapsulated in collagen microspheres. The effects of pre-differentiating the encapsulated MSC into chondrogenic lineages and different cell densities on cartilage repair were investigated in rabbits. Implantation of undifferentiated collagen-MSC microspheres formed hyaline-like cartilage rich in type II collagen and glycosaminoglycans (GAG) at 1month post-implantation. By 6months, hyaline cartilage rich in type II collagen and GAG, but negative for type I collagen, and partial zonal organization were found in both undifferentiated and chondrogenically differentiated groups in the high cell density group. The undifferentiated group and high cell density group significantly improved the O'Driscoll histological score. Moreover, the undifferentiated group significantly increased the GAG content. The mechanically differentiated group showed stiffer but thinner cartilage, while the undifferentiated group showed thicker but softer cartilage compared with their respective contra-lateral controls. This work suggests that a higher local cell density favors cartilage regeneration, regardless of the differentiation status of MSC, while the differentiation status of MSC does significantly affect regeneration outcomes.
Persistent Identifierhttp://hdl.handle.net/10722/202525
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLi, YYen_US
dc.contributor.authorCheng, HWen_US
dc.contributor.authorCheung, KMCen_US
dc.contributor.authorChan, Den_US
dc.contributor.authorChan, BPen_US
dc.date.accessioned2014-09-19T08:25:13Z-
dc.date.available2014-09-19T08:25:13Z-
dc.date.issued2014en_US
dc.identifier.citationActa Biomaterialia, 2014, v. 10, Issue 5, p. 1919-1929en_US
dc.identifier.urihttp://hdl.handle.net/10722/202525-
dc.description.abstractMesenchymal stem cells (MSC) hold promise for cartilage repair. A microencapsulation technique was previously established to entrap MSC in collagen microspheres, and the collagen fibrous meshwork was found to be an excellent scaffold for supporting MSC survival, growth and differentiation. This study investigates the importance of cell density and differentiation status of MSC-collagen microspheres in cartilage repair. MSC were isolated from rabbit bone marrow and encapsulated in collagen microspheres. The effects of pre-differentiating the encapsulated MSC into chondrogenic lineages and different cell densities on cartilage repair were investigated in rabbits. Implantation of undifferentiated collagen-MSC microspheres formed hyaline-like cartilage rich in type II collagen and glycosaminoglycans (GAG) at 1month post-implantation. By 6months, hyaline cartilage rich in type II collagen and GAG, but negative for type I collagen, and partial zonal organization were found in both undifferentiated and chondrogenically differentiated groups in the high cell density group. The undifferentiated group and high cell density group significantly improved the O'Driscoll histological score. Moreover, the undifferentiated group significantly increased the GAG content. The mechanically differentiated group showed stiffer but thinner cartilage, while the undifferentiated group showed thicker but softer cartilage compared with their respective contra-lateral controls. This work suggests that a higher local cell density favors cartilage regeneration, regardless of the differentiation status of MSC, while the differentiation status of MSC does significantly affect regeneration outcomes.en_US
dc.languageengen_US
dc.publisherElsevier. The Journal's web site is located at http://www.elsevier.com/locate/actabiomaten_US
dc.relation.ispartofActa Biomaterialiaen_US
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in <Journal title>. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in PUBLICATION, [VOL#, ISSUE#, (DATE)] DOI#en_US
dc.titleMesenchymal stem cell-collagen microspheres for articular cartilage repair: cell density and differentiation status.en_US
dc.typeArticleen_US
dc.identifier.emailLi, YY: cyyli@graduate.hku.hken_US
dc.identifier.emailCheng, HW: vernty@hku.hken_US
dc.identifier.emailCheung, KMC: cheungmc@hku.hken_US
dc.identifier.emailChan, D: chand@hku.hken_US
dc.identifier.emailChan, BP: bpchan@hkucc.hku.hken_US
dc.identifier.authorityCheung, KMC=rp00387en_US
dc.identifier.authorityChan, D=rp00540en_US
dc.identifier.authorityChan, BP=rp00087en_US
dc.identifier.doi10.1016/j.actbio.2014.01.002en_US
dc.identifier.pmid24418436-
dc.identifier.hkuros239580en_US
dc.identifier.hkuros239629-
dc.identifier.volume10, Issue 5en_US
dc.identifier.spage1919en_US
dc.identifier.epage1929en_US
dc.identifier.isiWOS:000335095300014-

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