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Conference Paper: Towards remyelination therapy: derivation of oligodendrocyte precursors from adult bone marrow stromal cells

TitleTowards remyelination therapy: derivation of oligodendrocyte precursors from adult bone marrow stromal cells
Authors
Issue Date2013
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/37090
Citation
The 11th European Meeting on Glial Cell Function in Health and Disease (GLIA), Berlin, Germany, 3-6 July 2013. In Glia, 2013, v. 61 n. Suppl. 1, p. S151, abstract no. T09-11B How to Cite?
AbstractMyelin damage and disorders cause axon to degenerate and result in severe loss of function. With an aim towards remyelination therapy, we attempted to direct differentiation of bone marrow stromal cells (BMSCs, adult rats) along the oligodendroglial lineage in vitro. BMSCs were first maintained in non-adherent culture to derive spheres of neural progenitors. These BMSC-derived neural progenitors were then induced to differentiate along oligodendorglial lineage in adherent culture supplemented with β-heregulin, PDGF-AA and bFGF. Oligodendrocyte precursors expressing the markers - NG2, Olig2, PDGFRa and Sox10, were detectable within two weeks and can be expanded in culture for more than 3 months with no observable decline in marker expression. To test for myelination capability, BMSC-derived oligodendrocyte precursor cells (OPCs) in a 2-week co-culture with dorsal root ganglion neurons extended myelin basic protein-positive processes along axons, suggesting maturation into myelinating oligodendrocytes. In vivo myelination by BM-OPCs was tested by exploitation of unmyelinated axons of retinal ganglion cells of adult rats. Myelin basic protein-positive processes were also observable along retinal axons by 8 weeks post-injection. Our findings indicate BMSCs as a possible source of OPCs for remyelination therapy.
DescriptionPoster Session II
Topic: T9 Neural stem/progenitor cells
Persistent Identifierhttp://hdl.handle.net/10722/202103
ISSN
2015 Impact Factor: 5.997
2015 SCImago Journal Rankings: 3.296

 

DC FieldValueLanguage
dc.contributor.authorTsui, YPen_US
dc.contributor.authorLi, SYen_US
dc.contributor.authorLo, ACYen_US
dc.contributor.authorChan, YSen_US
dc.contributor.authorShum, DKYen_US
dc.date.accessioned2014-08-21T08:03:50Z-
dc.date.available2014-08-21T08:03:50Z-
dc.date.issued2013en_US
dc.identifier.citationThe 11th European Meeting on Glial Cell Function in Health and Disease (GLIA), Berlin, Germany, 3-6 July 2013. In Glia, 2013, v. 61 n. Suppl. 1, p. S151, abstract no. T09-11Ben_US
dc.identifier.issn0894-1491-
dc.identifier.urihttp://hdl.handle.net/10722/202103-
dc.descriptionPoster Session II-
dc.descriptionTopic: T9 Neural stem/progenitor cells-
dc.description.abstractMyelin damage and disorders cause axon to degenerate and result in severe loss of function. With an aim towards remyelination therapy, we attempted to direct differentiation of bone marrow stromal cells (BMSCs, adult rats) along the oligodendroglial lineage in vitro. BMSCs were first maintained in non-adherent culture to derive spheres of neural progenitors. These BMSC-derived neural progenitors were then induced to differentiate along oligodendorglial lineage in adherent culture supplemented with β-heregulin, PDGF-AA and bFGF. Oligodendrocyte precursors expressing the markers - NG2, Olig2, PDGFRa and Sox10, were detectable within two weeks and can be expanded in culture for more than 3 months with no observable decline in marker expression. To test for myelination capability, BMSC-derived oligodendrocyte precursor cells (OPCs) in a 2-week co-culture with dorsal root ganglion neurons extended myelin basic protein-positive processes along axons, suggesting maturation into myelinating oligodendrocytes. In vivo myelination by BM-OPCs was tested by exploitation of unmyelinated axons of retinal ganglion cells of adult rats. Myelin basic protein-positive processes were also observable along retinal axons by 8 weeks post-injection. Our findings indicate BMSCs as a possible source of OPCs for remyelination therapy.-
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/37090-
dc.relation.ispartofGliaen_US
dc.rightsGlia. Copyright © John Wiley & Sons, Inc.-
dc.titleTowards remyelination therapy: derivation of oligodendrocyte precursors from adult bone marrow stromal cellsen_US
dc.typeConference_Paperen_US
dc.identifier.emailTsui, YP: alex2013@hku.hken_US
dc.identifier.emailLi, SY: sukyeeli@hku.hken_US
dc.identifier.emailLo, ACY: amylo@hkucc.hku.hken_US
dc.identifier.emailChan, YS: yschan@hku.hken_US
dc.identifier.emailShum, DKY: shumdkhk@hkucc.hku.hken_US
dc.identifier.authorityLo, ACY=rp00425en_US
dc.identifier.authorityChan, YS=rp00318en_US
dc.identifier.doi10.1002/glia.22530-
dc.identifier.hkuros235063en_US
dc.identifier.hkuros238151-
dc.identifier.volume61-
dc.identifier.issueSuppl. 1-
dc.identifier.spageS151, abstract no. T09-11B-
dc.identifier.epageS151, abstract no. T09-11B-
dc.publisher.placeUnited States-

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