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Conference Paper: Attenuation Of Cigarette Smoke-Induced Cardiac Dysfunction And Inflammation By Mesenchymal Stem Cells In Rat

TitleAttenuation Of Cigarette Smoke-Induced Cardiac Dysfunction And Inflammation By Mesenchymal Stem Cells In Rat
Authors
Issue Date2014
PublisherAmerican Thoracic Society. The Journal's web site is located at http://ajrccm.atsjournals.org
Citation
The 2014 International Conference of the American Thoracic Society (ATS), San Diego, CA., 16-21 May 2014. In American Journal of Respiratory and Critical Care Medicine, 2014, v. 189, abstract no. A6550 How to Cite?
AbstractRationale: Cigarette smoke (CS) is recognized as a major cause of cardiovascular disease (CVD), particularly in those with chronic obstructive pulmonary disease (COPD). Oxidative stress and inflammatory responses may play an important role in the pathophysiological processes of CS-induced cardiac damage. Stem cell therapy has the potential to be a promising treatment in the field of regenerative cardiovascular medicine. Mesenchymal stem cells (MSC), was regarded as a leading candidate for cell-based therapy for myocardial infarction. The aim of this study is to investigate the effect of bone marrow-derived MSCs (BM-MSC) and induced pluripotent stem cell-derived MSC (IPSC-MSC) on cardiac function and inflammation in the cigarette smoke-exposed rat model. Methods: Male Sprague-Dawley rats (aged 6-7 weeks) were randomly divided into sham air (SA) group, cigarette smoke (CS) group, IPSC-MSC treatment (IP/CS) group and BM-MSC treatment (BM/CS) group respectively. All the animals were exposed to 4% CS except SA group to fresh air for 1h each day for 56 days in the ventilated chambers. IPSC-MSCs or BM-MSCs (3×10 cells) were injected intravenously 6 via tail vein in IP/CS or BM/CS group on day 29 and day 43. Animals were anaesthetized 24 hours after the last exposure for cardiac function examination by echocardiography. Cardiac levels of inflammatory and anti-inflammatory markers were measured by ELISA. Results: From echocardiograph, IPSC-MSC had a better trend of reversing the CS-induced reduction in cardiac function by elevating left ventricular ejection fraction (LVEF) and fractional shortening (FS) than BM-MSC. Both IPSC-MSC and BM-MSC administrations were able to attenuate the CS-induced elevation of cardiac proinflammatory cytokines, tumor necrosis factor-alpha (TNF-α) and cytokine-induced neutrophil chemoattractant-1 (CINC-1, resemble to human IL-8). IPSC-MSC also significantly restored CS-induced reduction of cardiac interleukin-10 (IL-10) and adiponectin, while BM-MSC had no such effect. Conclusion: Our findings found that IPSC-MSC treatment could alleviate the CS-induced cardiac dysfunction and attenuate cardiac inflammatory changes by inhibiting proinflammatory cytokines and restoring anti-inflammatory mediators in the CS-exposed rat model, suggesting the cardio-protective effects of IPSC-MSC therapy. This abstract is funded by: Hong Kong RGC_GRF 2010-2011 (HKU 774410M)
DescriptionPoster Discussion Session: D110 Chronic Lung Disease Throughout Life: From Priming in Utero to the Aging Lung
Persistent Identifierhttp://hdl.handle.net/10722/202101
ISSN
2015 Impact Factor: 13.118
2015 SCImago Journal Rankings: 5.832

 

DC FieldValueLanguage
dc.contributor.authorLiang, Yen_US
dc.contributor.authorLi, Xen_US
dc.contributor.authorZhang, Yen_US
dc.contributor.authorIp, MSMen_US
dc.contributor.authorTse, HFen_US
dc.contributor.authorLian, Qen_US
dc.contributor.authorMak, JCWen_US
dc.date.accessioned2014-08-21T08:03:50Z-
dc.date.available2014-08-21T08:03:50Z-
dc.date.issued2014en_US
dc.identifier.citationThe 2014 International Conference of the American Thoracic Society (ATS), San Diego, CA., 16-21 May 2014. In American Journal of Respiratory and Critical Care Medicine, 2014, v. 189, abstract no. A6550en_US
dc.identifier.issn1073-449X-
dc.identifier.urihttp://hdl.handle.net/10722/202101-
dc.descriptionPoster Discussion Session: D110 Chronic Lung Disease Throughout Life: From Priming in Utero to the Aging Lung-
dc.description.abstractRationale: Cigarette smoke (CS) is recognized as a major cause of cardiovascular disease (CVD), particularly in those with chronic obstructive pulmonary disease (COPD). Oxidative stress and inflammatory responses may play an important role in the pathophysiological processes of CS-induced cardiac damage. Stem cell therapy has the potential to be a promising treatment in the field of regenerative cardiovascular medicine. Mesenchymal stem cells (MSC), was regarded as a leading candidate for cell-based therapy for myocardial infarction. The aim of this study is to investigate the effect of bone marrow-derived MSCs (BM-MSC) and induced pluripotent stem cell-derived MSC (IPSC-MSC) on cardiac function and inflammation in the cigarette smoke-exposed rat model. Methods: Male Sprague-Dawley rats (aged 6-7 weeks) were randomly divided into sham air (SA) group, cigarette smoke (CS) group, IPSC-MSC treatment (IP/CS) group and BM-MSC treatment (BM/CS) group respectively. All the animals were exposed to 4% CS except SA group to fresh air for 1h each day for 56 days in the ventilated chambers. IPSC-MSCs or BM-MSCs (3×10 cells) were injected intravenously 6 via tail vein in IP/CS or BM/CS group on day 29 and day 43. Animals were anaesthetized 24 hours after the last exposure for cardiac function examination by echocardiography. Cardiac levels of inflammatory and anti-inflammatory markers were measured by ELISA. Results: From echocardiograph, IPSC-MSC had a better trend of reversing the CS-induced reduction in cardiac function by elevating left ventricular ejection fraction (LVEF) and fractional shortening (FS) than BM-MSC. Both IPSC-MSC and BM-MSC administrations were able to attenuate the CS-induced elevation of cardiac proinflammatory cytokines, tumor necrosis factor-alpha (TNF-α) and cytokine-induced neutrophil chemoattractant-1 (CINC-1, resemble to human IL-8). IPSC-MSC also significantly restored CS-induced reduction of cardiac interleukin-10 (IL-10) and adiponectin, while BM-MSC had no such effect. Conclusion: Our findings found that IPSC-MSC treatment could alleviate the CS-induced cardiac dysfunction and attenuate cardiac inflammatory changes by inhibiting proinflammatory cytokines and restoring anti-inflammatory mediators in the CS-exposed rat model, suggesting the cardio-protective effects of IPSC-MSC therapy. This abstract is funded by: Hong Kong RGC_GRF 2010-2011 (HKU 774410M)-
dc.languageengen_US
dc.publisherAmerican Thoracic Society. The Journal's web site is located at http://ajrccm.atsjournals.org-
dc.relation.ispartofAmerican Journal of Respiratory and Critical Care Medicineen_US
dc.titleAttenuation Of Cigarette Smoke-Induced Cardiac Dysfunction And Inflammation By Mesenchymal Stem Cells In Raten_US
dc.typeConference_Paperen_US
dc.identifier.emailIp, MSM: msmip@hku.hken_US
dc.identifier.emailTse, HF: hftse@hkucc.hku.hken_US
dc.identifier.emailLian, Q: qzlian@hkucc.hku.hken_US
dc.identifier.emailMak, JCW: judithmak@hku.hken_US
dc.identifier.authorityIp, MSM=rp00347en_US
dc.identifier.authorityTse, HF=rp00428en_US
dc.identifier.authorityLian, Q=rp00267en_US
dc.identifier.authorityMak, JCW=rp00352en_US
dc.identifier.hkuros234877en_US
dc.identifier.volume189en_US
dc.publisher.placeUnited States-

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