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Conference Paper: Oleanolic Acid Delivery using Biodegradable Nanoparticles for Cancer Therapy

TitleOleanolic Acid Delivery using Biodegradable Nanoparticles for Cancer Therapy
Authors
Issue Date2014
Citation
The 2014 International Colloid and Interface Science Symposium (UK Colloids 2014), London, UK., 6-9 July 2014. In Conference Booklet, 2014, p. 267, abstract no. P45 How to Cite?
AbstractOleanolic acid (OA) is a naturally occurring pentacyclic triterpenoid widely found in Chinese herbs. It has been extensively studied owing to the beneficial effects such as hepatoprotection, anti-inflammatory and it is recently found to have anti-tumor ability. However, resembling the other hydrophobic drugs, it has poor water solubility and therefore very limited intestinal absorption when administrated orally. Investigations concerning the delivery of OA have been carried out to enhance its dissolution and bioavailability. Nanoparticulate systems involve the reduction of drug particle size into nano-scale thereby increasing the interfacial surface area. As a result, absorption of drug in the body system could be enhanced. Biodegradable polymer-based system can be metabolized by the body system into harmless compounds so it is regarded safe and promising. This study was conducted to evaluate the efficacy of four types of biodegradable copolymers as potential OA delivery nanoparticulate system. Co-polymers consisting a hydrophilic block: poly(ethylene) glycol (PEG) and a hydrophobic block of either poly(lactic-co-glycolic) acid (PLGA) or poly(lactic acid) (PLA) were used to fabricate a novel nanoformulation to improve OA bioavailability. The OA-loaded mPEG-(D,L)PLA, mPEG-(L)PLA, mPEG-(D,L)PLGA and mPEG- (L)PLGA nanoparticles (NPs), prepared by nanoprecipitation, were observed to be spherical in shape under transmission electron microscope. Results indicated that narrow size distributed NPs with mean hydrodynamic diameter of 200±16.0, 233±13.9, 211±10.1 and 229±10.1nm respectively were obtained. mPEG-(D,L)PLA NPs attained the highest encapsulation of OA with 75.8% efficiency while mPEG-(L)PLGA NPs displayed the lowest encapsulation efficiency at 40.8%. The in vitro anti-tumoral activity was evaluated on A549, lung carcinoma epithelial cell lines using MTT assay. All the four types of OA-loaded NPs demonstrated better anti-cancer ability than saturated OA in medium. Moreover, NPs without OA exhibited significantly lower cytotoxicity towards A549 indicating induced cell death was due to the effective delivery of OA as an anti-cancer therapeutic agent.
DescriptionPoster Presentation
Persistent Identifierhttp://hdl.handle.net/10722/201346

 

DC FieldValueLanguage
dc.contributor.authorMan, KWDen_US
dc.contributor.authorKwok, PCLen_US
dc.contributor.authorLam, JKWen_US
dc.date.accessioned2014-08-21T07:24:50Z-
dc.date.available2014-08-21T07:24:50Z-
dc.date.issued2014en_US
dc.identifier.citationThe 2014 International Colloid and Interface Science Symposium (UK Colloids 2014), London, UK., 6-9 July 2014. In Conference Booklet, 2014, p. 267, abstract no. P45en_US
dc.identifier.urihttp://hdl.handle.net/10722/201346-
dc.descriptionPoster Presentation-
dc.description.abstractOleanolic acid (OA) is a naturally occurring pentacyclic triterpenoid widely found in Chinese herbs. It has been extensively studied owing to the beneficial effects such as hepatoprotection, anti-inflammatory and it is recently found to have anti-tumor ability. However, resembling the other hydrophobic drugs, it has poor water solubility and therefore very limited intestinal absorption when administrated orally. Investigations concerning the delivery of OA have been carried out to enhance its dissolution and bioavailability. Nanoparticulate systems involve the reduction of drug particle size into nano-scale thereby increasing the interfacial surface area. As a result, absorption of drug in the body system could be enhanced. Biodegradable polymer-based system can be metabolized by the body system into harmless compounds so it is regarded safe and promising. This study was conducted to evaluate the efficacy of four types of biodegradable copolymers as potential OA delivery nanoparticulate system. Co-polymers consisting a hydrophilic block: poly(ethylene) glycol (PEG) and a hydrophobic block of either poly(lactic-co-glycolic) acid (PLGA) or poly(lactic acid) (PLA) were used to fabricate a novel nanoformulation to improve OA bioavailability. The OA-loaded mPEG-(D,L)PLA, mPEG-(L)PLA, mPEG-(D,L)PLGA and mPEG- (L)PLGA nanoparticles (NPs), prepared by nanoprecipitation, were observed to be spherical in shape under transmission electron microscope. Results indicated that narrow size distributed NPs with mean hydrodynamic diameter of 200±16.0, 233±13.9, 211±10.1 and 229±10.1nm respectively were obtained. mPEG-(D,L)PLA NPs attained the highest encapsulation of OA with 75.8% efficiency while mPEG-(L)PLGA NPs displayed the lowest encapsulation efficiency at 40.8%. The in vitro anti-tumoral activity was evaluated on A549, lung carcinoma epithelial cell lines using MTT assay. All the four types of OA-loaded NPs demonstrated better anti-cancer ability than saturated OA in medium. Moreover, NPs without OA exhibited significantly lower cytotoxicity towards A549 indicating induced cell death was due to the effective delivery of OA as an anti-cancer therapeutic agent.-
dc.languageengen_US
dc.relation.ispartofInternational Colloid and Interface Science Symposium, UK Colloids 2014en_US
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.titleOleanolic Acid Delivery using Biodegradable Nanoparticles for Cancer Therapyen_US
dc.typeConference_Paperen_US
dc.identifier.emailKwok, PCL: pclkwok@hku.hken_US
dc.identifier.emailLam, JKW: jkwlam@hku.hken_US
dc.identifier.authorityKwok, PCL=rp01540en_US
dc.identifier.authorityLam, JKW=rp01346en_US
dc.description.naturepublished_or_final_version-
dc.identifier.hkuros233297en_US
dc.identifier.hkuros238795-
dc.identifier.spage267, abstract no. P45-
dc.identifier.epage267, abstract no. P45-

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