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Conference Paper: Effect of L-citrulline on nitrate tolerance: role of arginase

TitleEffect of L-citrulline on nitrate tolerance: role of arginase
Authors
KeywordsPharmacy and pharmacology environmental studies
Toxicology and environmental safety
Issue Date2014
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/PTO
Citation
The 17th World Congress of Basic and Clinical Pharmacology (WCP2014), Cape Town, South Africa, 13-18 July 2014. In Basic & Clinical Pharmacology & Toxicology, 2014, v. 115 suppl. s1, p. 20, abstract no. 63 How to Cite?
AbstractBACKGROUND: Chronic administration of nitroglycerin (GTN) leads to nitrate tolerance, which is associated with increased arginase II activity. Supplementation of L-arginine appears to reduce nitrate tolerance. Experiments were designed to examine whether or not L-citrulline, the precursor and the metabolite of L-arginine, prevents the development of nitrate tolerance, and whether or not arginase plays a role. METHODS: Human umbilical vein endothelial cells (HUVEC) were exposed to nitroglycerin (GTN; 10 lM), in the absence and presence of L-arginine (1 mM), L-citrulline (10 mM) and/or L-norvaline (10 mM), for one and 24 h. They were then stimulated with GTN (100 lM) and the amount of cyclic guanosine triphosphate (cyclic GMP) release was measured by enzyme immunoassay. The protein expressions and the activities of arginases were examined by Western blotting and colorimetric assay, respectively. RESULTS: Prolonged (24 h), but not short-term (1 h), incubation of HUVECs with exogenous GTN caused a significant reduction of the release of cyclic GMP caused by the acute administration of the nitrate. This reduction was not affected significantly by L-citrulline or L-norvaline, while L-arginine appeared to blunt the reduction. Prolonged incubation with exogenous GTN also caused a significant increase in arginase activity, which was prevented by L-norvaline, in control cell cultures or in those co-incubated with L-citrulline, but not with L-arginine. The different incubations did not significantly affect the protein presence of arginase II, in the absence of L-norvaline; the presence of arginase I was not detectable in HUVECs with Western blotting. CONCLUSIONS: These experiments show minimal involvement of arginase II, L-citrulline and L-arginine in tolerance that develops in HUVECs following 24 h of exposure to GTN.
DescriptionSession - Cardiovascular Pharmacology
This journal suppl. entitled: Special Issue: Abstracts of the 17th World Congress of Basic and Clinical Pharmacology ... 2014
Persistent Identifierhttp://hdl.handle.net/10722/201342
ISSN
2015 Impact Factor: 3.097
2015 SCImago Journal Rankings: 0.539

 

DC FieldValueLanguage
dc.contributor.authorLeung, Sen_US
dc.contributor.authorShum, KPWen_US
dc.contributor.authorKaesemeyer, WHen_US
dc.contributor.authorVanhoutte, Pen_US
dc.contributor.authorMan, Ren_US
dc.date.accessioned2014-08-21T07:24:50Z-
dc.date.available2014-08-21T07:24:50Z-
dc.date.issued2014en_US
dc.identifier.citationThe 17th World Congress of Basic and Clinical Pharmacology (WCP2014), Cape Town, South Africa, 13-18 July 2014. In Basic & Clinical Pharmacology & Toxicology, 2014, v. 115 suppl. s1, p. 20, abstract no. 63en_US
dc.identifier.issn1742-7835-
dc.identifier.urihttp://hdl.handle.net/10722/201342-
dc.descriptionSession - Cardiovascular Pharmacology-
dc.descriptionThis journal suppl. entitled: Special Issue: Abstracts of the 17th World Congress of Basic and Clinical Pharmacology ... 2014-
dc.description.abstractBACKGROUND: Chronic administration of nitroglycerin (GTN) leads to nitrate tolerance, which is associated with increased arginase II activity. Supplementation of L-arginine appears to reduce nitrate tolerance. Experiments were designed to examine whether or not L-citrulline, the precursor and the metabolite of L-arginine, prevents the development of nitrate tolerance, and whether or not arginase plays a role. METHODS: Human umbilical vein endothelial cells (HUVEC) were exposed to nitroglycerin (GTN; 10 lM), in the absence and presence of L-arginine (1 mM), L-citrulline (10 mM) and/or L-norvaline (10 mM), for one and 24 h. They were then stimulated with GTN (100 lM) and the amount of cyclic guanosine triphosphate (cyclic GMP) release was measured by enzyme immunoassay. The protein expressions and the activities of arginases were examined by Western blotting and colorimetric assay, respectively. RESULTS: Prolonged (24 h), but not short-term (1 h), incubation of HUVECs with exogenous GTN caused a significant reduction of the release of cyclic GMP caused by the acute administration of the nitrate. This reduction was not affected significantly by L-citrulline or L-norvaline, while L-arginine appeared to blunt the reduction. Prolonged incubation with exogenous GTN also caused a significant increase in arginase activity, which was prevented by L-norvaline, in control cell cultures or in those co-incubated with L-citrulline, but not with L-arginine. The different incubations did not significantly affect the protein presence of arginase II, in the absence of L-norvaline; the presence of arginase I was not detectable in HUVECs with Western blotting. CONCLUSIONS: These experiments show minimal involvement of arginase II, L-citrulline and L-arginine in tolerance that develops in HUVECs following 24 h of exposure to GTN.-
dc.languageengen_US
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/PTO-
dc.relation.ispartofBasic & Clinical Pharmacology & Toxicologyen_US
dc.rightsThe definitive version is available at www.blackwell-synergy.com-
dc.subjectPharmacy and pharmacology environmental studies-
dc.subjectToxicology and environmental safety-
dc.titleEffect of L-citrulline on nitrate tolerance: role of arginaseen_US
dc.typeConference_Paperen_US
dc.identifier.emailLeung, S: swsleung@hku.hken_US
dc.identifier.emailVanhoutte, P: vanhoutt@hku.hken_US
dc.identifier.emailMan, R: rykman@hkucc.hku.hken_US
dc.identifier.authorityLeung, S=rp00235en_US
dc.identifier.authorityVanhoutte, P=rp00238en_US
dc.identifier.authorityMan, R=rp00236en_US
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1111/bcpt.12259_1-
dc.identifier.hkuros233052en_US
dc.identifier.volume115-
dc.identifier.issuesuppl. s1-
dc.identifier.spage20, abstract no. 63-
dc.identifier.epage20, abstract no. 63-
dc.publisher.placeUnited Kingdom-

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