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Conference Paper: Development of three-dimesional system for culturing notochordal cells

TitleDevelopment of three-dimesional system for culturing notochordal cells
Authors
Issue Date2013
Citation
The 9th Pan Pacific Connective Tissue Societies Symposium (PPCTSS 2013), Hong Kong, China, 24-27 November 2013. In the Program Book of the 9th Pan Pacific Connective Tissue Societies Symposium, 2013, p. abstract no. 0054 How to Cite?
AbstractBackground: Notochordal cells (NCCs) appear to play an important role in the formation of the spine and loss of them is related to disc degeneration. Recently, NCCs have drawn increasing attentions from developmental biology, stem cells research and tissue engineering as they are important in development and maintenance of NP. In our group, NCCs have been isolated from Foxa2mNE– Cre/Z/EG heterozygous embryos by identifying EGFP signal using FACS. However, the yield of EGFP positive cells is low (1%). These cells can be cultured as monolayer but all EGFP signals are gone in one month’s time. As a result, development of a better culture system able to maintain their phenotype is warranted. Methods: FACS-sorted NCCs or notochord segments at the anterior, trunk and posterior regions were microencapsulated in type I collagen microspheres and cultured for up to 1 month before characterization for survival and phenotype maintenance. Results: We demonstrated that FACS-sorted NCCs maintained their survival within the collagen microspheres for at least 4 weeks. However, most GFP cells showed elongated morphology. When the notochord segments were cultured in collagen microspheres, clusters of round GFP positive cells co-expressing with major NCC markers were found after 1 month, suggesting that NCC phenotype was maintained. Longer period of culture and the role of niche cells present in the notochord in supporting NCC maintenance are warranted. Conclusion: Type I collagen microspheres present a potential 3D culture system for FACS-sorted NCCs or NC segments.
DescriptionConference Theme: The Extracellular Matrix Niche
Poster Presentation
The Program can be viewed at: http://ppctss2013.org/Online%20Program%20Book.pdf
Persistent Identifierhttp://hdl.handle.net/10722/201170

 

DC FieldValueLanguage
dc.contributor.authorYuan, Men_US
dc.contributor.authorYeung, CWen_US
dc.contributor.authorAu, YKen_US
dc.contributor.authorGao, Yen_US
dc.contributor.authorChan, Den_US
dc.contributor.authorCheah, KSEen_US
dc.contributor.authorChan, BPen_US
dc.date.accessioned2014-08-21T07:16:30Z-
dc.date.available2014-08-21T07:16:30Z-
dc.date.issued2013en_US
dc.identifier.citationThe 9th Pan Pacific Connective Tissue Societies Symposium (PPCTSS 2013), Hong Kong, China, 24-27 November 2013. In the Program Book of the 9th Pan Pacific Connective Tissue Societies Symposium, 2013, p. abstract no. 0054en_US
dc.identifier.urihttp://hdl.handle.net/10722/201170-
dc.descriptionConference Theme: The Extracellular Matrix Niche-
dc.descriptionPoster Presentation-
dc.descriptionThe Program can be viewed at: http://ppctss2013.org/Online%20Program%20Book.pdf-
dc.description.abstractBackground: Notochordal cells (NCCs) appear to play an important role in the formation of the spine and loss of them is related to disc degeneration. Recently, NCCs have drawn increasing attentions from developmental biology, stem cells research and tissue engineering as they are important in development and maintenance of NP. In our group, NCCs have been isolated from Foxa2mNE– Cre/Z/EG heterozygous embryos by identifying EGFP signal using FACS. However, the yield of EGFP positive cells is low (1%). These cells can be cultured as monolayer but all EGFP signals are gone in one month’s time. As a result, development of a better culture system able to maintain their phenotype is warranted. Methods: FACS-sorted NCCs or notochord segments at the anterior, trunk and posterior regions were microencapsulated in type I collagen microspheres and cultured for up to 1 month before characterization for survival and phenotype maintenance. Results: We demonstrated that FACS-sorted NCCs maintained their survival within the collagen microspheres for at least 4 weeks. However, most GFP cells showed elongated morphology. When the notochord segments were cultured in collagen microspheres, clusters of round GFP positive cells co-expressing with major NCC markers were found after 1 month, suggesting that NCC phenotype was maintained. Longer period of culture and the role of niche cells present in the notochord in supporting NCC maintenance are warranted. Conclusion: Type I collagen microspheres present a potential 3D culture system for FACS-sorted NCCs or NC segments.-
dc.languageengen_US
dc.relation.ispartofPan Pacific Connective Tissue Societies Symposium (PPCTSS)en_US
dc.titleDevelopment of three-dimesional system for culturing notochordal cellsen_US
dc.typeConference_Paperen_US
dc.identifier.emailYuan, M: mintyuan@hku.hken_US
dc.identifier.emailYeung, CW: ccwyeung@hku.hken_US
dc.identifier.emailAu, YK: h0294066@hku.hken_US
dc.identifier.emailGao, Y: genegao@hku.hken_US
dc.identifier.emailChan, D: chand@hku.hken_US
dc.identifier.emailCheah, KSE: hrmbdkc@hku.hken_US
dc.identifier.emailChan, BP: bpchan@hkucc.hku.hken_US
dc.identifier.authorityChan, D=rp00540en_US
dc.identifier.authorityCheah, KSE=rp00342en_US
dc.identifier.authorityChan, BP=rp00087en_US
dc.identifier.hkuros234171en_US
dc.identifier.hkuros239667-

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