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Article: High-resolution detection of recurrent aberrations in lung adenocarcinomas by array comparative genomic hybridization and expression analysis of selective genes by quantitative PCR

TitleHigh-resolution detection of recurrent aberrations in lung adenocarcinomas by array comparative genomic hybridization and expression analysis of selective genes by quantitative PCR
Authors
Issue Date2014
PublisherSpandidos Publications. The Journal's web site is located at http://www.spandidos-publications.com/ijo/
Citation
International Journal of Oncology, 2014, v. 44 n. 6, p. 2068-2076 How to Cite?
AbstractGenomic abnormalities are the hallmark of cancers and may harbor potential candidate genes important for cancer development and progression. We performed array comparative genomic hybridization (array CGH) on 36 cases of primary lung adenocarcinoma (AD) using an array containing 2621 BAC or PAC clones spanning the genome at an average interval of 1 Mb. Array CGH identified the commonest aberrations consisting of DNA gains within 1p, 1q, 5p, 5q, 7p, 7q, 8q, 11q, 12p, 13q, 16p, 17q, 20q, and losses with 6q, 9p, 10q and 18q. High-level copy gains involved mainly 7p21-p15 and 20q13.3. Dual color fluorescence in situ hybridization (FISH) was performed on a selective locus for validation of array CGH results. Genomic aberrations were compared with different clinicopathological features and a trend of higher number of aberrations in tumors with aggressive phenotypes and current tobacco exposure was identified. According to array CGH data, 23 candidate genes were selected for quantitative PCR (qPCR) analysis. The concordance observed between the genomic and expression changes in most of the genes suggested that they could be candidate cancer-related genes that contributed to the development of lung AD.
Persistent Identifierhttp://hdl.handle.net/10722/201065
ISSN
2015 Impact Factor: 3.018
2015 SCImago Journal Rankings: 1.270

 

DC FieldValueLanguage
dc.contributor.authorZhu, H-
dc.contributor.authorWong, MP-
dc.contributor.authorTin, VPC-
dc.date.accessioned2014-08-21T07:12:01Z-
dc.date.available2014-08-21T07:12:01Z-
dc.date.issued2014-
dc.identifier.citationInternational Journal of Oncology, 2014, v. 44 n. 6, p. 2068-2076-
dc.identifier.issn1019-6439-
dc.identifier.urihttp://hdl.handle.net/10722/201065-
dc.description.abstractGenomic abnormalities are the hallmark of cancers and may harbor potential candidate genes important for cancer development and progression. We performed array comparative genomic hybridization (array CGH) on 36 cases of primary lung adenocarcinoma (AD) using an array containing 2621 BAC or PAC clones spanning the genome at an average interval of 1 Mb. Array CGH identified the commonest aberrations consisting of DNA gains within 1p, 1q, 5p, 5q, 7p, 7q, 8q, 11q, 12p, 13q, 16p, 17q, 20q, and losses with 6q, 9p, 10q and 18q. High-level copy gains involved mainly 7p21-p15 and 20q13.3. Dual color fluorescence in situ hybridization (FISH) was performed on a selective locus for validation of array CGH results. Genomic aberrations were compared with different clinicopathological features and a trend of higher number of aberrations in tumors with aggressive phenotypes and current tobacco exposure was identified. According to array CGH data, 23 candidate genes were selected for quantitative PCR (qPCR) analysis. The concordance observed between the genomic and expression changes in most of the genes suggested that they could be candidate cancer-related genes that contributed to the development of lung AD.-
dc.languageeng-
dc.publisherSpandidos Publications. The Journal's web site is located at http://www.spandidos-publications.com/ijo/-
dc.relation.ispartofInternational Journal of Oncology-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.titleHigh-resolution detection of recurrent aberrations in lung adenocarcinomas by array comparative genomic hybridization and expression analysis of selective genes by quantitative PCR-
dc.typeArticle-
dc.identifier.emailWong, MP: mwpik@hku.hk-
dc.identifier.emailTin, VPC: pctin@hku.hk-
dc.identifier.authorityWong, MP=rp00348-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.3892/ijo.2014.2384-
dc.identifier.pmid24728343-
dc.identifier.hkuros234673-
dc.identifier.volume44-
dc.identifier.issue6-
dc.identifier.spage2068-
dc.identifier.epage2076-
dc.publisher.placeGreece-

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